Restriction map for
pFN21AE5631
(Linear) (Six-base) MAPSORT of: KIEE5631.seq Check: 4405 from: 1 to: 1431
KIEE5631.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 216 1431
Size: 216 1215
AleI CACnn'nnGTG
Cuts at: 0 598 829 1431
Size: 598 231 602
Fragments arranged by size:
602 598 231
AlwNI CAG_nnn'CTG
Cuts at: 0 207 629 1098 1228 1257 1431
Size: 207 422 469 130 29 174
Fragments arranged by size:
469 422 207 174 130 29
ApaI G_GGCC'C
Cuts at: 0 639 717 1431
Size: 639 78 714
Fragments arranged by size:
714 639 78
ApaLI G'TGCA_C
Cuts at: 0 1018 1431
Size: 1018 413
AvaI C'yCGr_G
Cuts at: 0 180 379 772 1003 1138 1431
Size: 180 199 393 231 135 293
Fragments arranged by size:
393 293 231 199 180 135
BamHI G'GATC_C
Cuts at: 0 1242 1431
Size: 1242 189
BanI G'GyrC_C
Cuts at: 0 28 1088 1378 1431
Size: 28 1060 290 53
Fragments arranged by size:
1060 290 53 28
BanII G_rGCy'C
Cuts at: 0 7 47 446 639 717 1431
Size: 7 40 399 193 78 714
Fragments arranged by size:
714 399 193 78 40 7
BbeI G_GCGC'C
Cuts at: 0 32 1382 1431
Size: 32 1350 49
Fragments arranged by size:
1350 49 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 484 1118 1431
Size: 484 634 313
Fragments arranged by size:
634 484 313
BglI GCCn_nnn'nGGC
Cuts at: 0 233 817 1319 1431
Size: 233 584 502 112
Fragments arranged by size:
584 502 233 112
BlpI GC'TnA_GC
Cuts at: 0 1258 1431
Size: 1258 173
Bme1580I G_kGCm'C
Cuts at: 0 639 717 1022 1431
Size: 639 78 305 409
Fragments arranged by size:
639 409 305 78
BmgBI CAC'GTC
Cuts at: 0 1284 1431
Size: 1284 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 630 835 1431
Size: 475 155 205 596
Fragments arranged by size:
596 475 205 155
Bpu10I CC'TnA_GC
Cuts at: 0 421 1405 1431
Size: 421 984 26
Fragments arranged by size:
984 421 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 192 1170 1408 1431
Size: 192 978 238 23
Fragments arranged by size:
978 238 192 23
BsaAI yAC'GTr
Cuts at: 0 357 546 1431
Size: 357 189 885
Fragments arranged by size:
885 357 189
BsaHI Gr'CG_yC
Cuts at: 0 29 1379 1431
Size: 29 1350 52
Fragments arranged by size:
1350 52 29
BsaWI w'CCGG_w
Cuts at: 0 332 580 1431
Size: 332 248 851
Fragments arranged by size:
851 332 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 1309 1339 1431
Size: 88 30 1191 30 92
Fragments arranged by size:
1191 92 88 30 30
BseYI C'CCAG_C
Cuts at: 0 622 692 762 1054 1431
Size: 622 70 70 292 377
Fragments arranged by size:
622 377 292 70 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1390 1431
Size: 1390 41
BsiEI CG_ry'CG
Cuts at: 0 893 1431
Size: 893 538
BsiHKAI G_wGCw'C
Cuts at: 0 7 344 1022 1309 1431
Size: 7 337 678 287 122
Fragments arranged by size:
678 337 287 122 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 47 344 446 639 717 1022 1309
Size: 7 40 297 102 193 78 305 287
Cuts at: 1309 1431
Size: 122
Fragments arranged by size:
305 297 287 193 122 102 78 40
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 1431
Size: 107 1324
BsrBI CCG'CTC
Cuts at: 0 668 1431
Size: 668 763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1095 1431
Size: 1095 336
BstEII G'GTnAC_C
Cuts at: 0 840 1069 1431
Size: 840 229 362
Fragments arranged by size:
840 362 229
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1284 1431
Size: 831 453 147
Fragments arranged by size:
831 453 147
BstYI r'GATC_y
Cuts at: 0 1242 1431
Size: 1242 189
BtgI C'CryG_G
Cuts at: 0 251 410 599 953 1331 1431
Size: 251 159 189 354 378 100
Fragments arranged by size:
378 354 251 189 159 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 1431
Size: 507 924
BtsI GCAGTG_nn'
Cuts at: 0 509 1244 1431
Size: 509 735 187
Fragments arranged by size:
735 509 187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 415 450 1431
Size: 415 35 981
Fragments arranged by size:
981 415 35
DraIII CAC_nnn'GTG
Cuts at: 0 1017 1431
Size: 1017 414
DrdI GACnn_nn'nnGTC
Cuts at: 0 457 547 1431
Size: 457 90 884
Fragments arranged by size:
884 457 90
EaeI y'GGCC_r
Cuts at: 0 890 1343 1391 1431
Size: 890 453 48 40
Fragments arranged by size:
890 453 48 40
EagI C'GGCC_G
Cuts at: 0 890 1431
Size: 890 541
EarI CTCTTCn'nnn_
Cuts at: 0 480 661 1431
Size: 480 181 770
Fragments arranged by size:
770 480 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 934 1431
Size: 934 497
EcoICRI GAG'CTC
Cuts at: 0 5 1431
Size: 5 1426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 630 835 1431
Size: 475 155 205 596
Fragments arranged by size:
596 475 205 155
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 658 1220 1431
Size: 199 459 562 211
Fragments arranged by size:
562 459 211 199
EcoO109I rG'GnC_Cy
Cuts at: 0 177 204 611 635 780 1134 1320 1431
Size: 177 27 407 24 145 354 186 111
Fragments arranged by size:
407 354 186 177 145 111 27 24
HaeII r_GCGC'y
Cuts at: 0 32 1265 1382 1431
Size: 32 1233 117 49
Fragments arranged by size:
1233 117 49 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 603 635 1431
Size: 202 32 369 32 796
Fragments arranged by size:
796 369 202 32 32
KasI G'GCGC_C
Cuts at: 0 28 1378 1431
Size: 28 1350 53
Fragments arranged by size:
1350 53 28
MscI TGG'CCA
Cuts at: 0 1393 1431
Size: 1393 38
MslI CAynn'nnrTG
Cuts at: 0 61 598 829 928 1282 1431
Size: 61 537 231 99 354 149
Fragments arranged by size:
537 354 231 149 99 61
MspA1I CmG'CkG
Cuts at: 0 629 889 984 1095 1257 1341 1431
Size: 629 260 95 111 162 84 90
Fragments arranged by size:
629 260 162 111 95 90 84
NarI GG'CG_CC
Cuts at: 0 29 1379 1431
Size: 29 1350 52
Fragments arranged by size:
1350 52 29
NcoI C'CATG_G
Cuts at: 0 410 1331 1431
Size: 410 921 100
Fragments arranged by size:
921 410 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 438 1431
Size: 207 231 993
Fragments arranged by size:
993 231 207
PfoI T'CCnGG_A
Cuts at: 0 194 446 1130 1431
Size: 194 252 684 301
Fragments arranged by size:
684 301 252 194
PmlI CAC'GTG
Cuts at: 0 357 1431
Size: 357 1074
PpuMI rG'GwC_Cy
Cuts at: 0 204 611 1134 1431
Size: 204 407 523 297
Fragments arranged by size:
523 407 297 204
PspOMI G'GGCC_C
Cuts at: 0 635 713 1431
Size: 635 78 718
Fragments arranged by size:
718 635 78
PspXI vC'TCGA_Gb
Cuts at: 0 1138 1431
Size: 1138 293
PstI C_TGCA'G
Cuts at: 0 161 1431
Size: 161 1270
PvuII CAG'CTG
Cuts at: 0 629 984 1095 1431
Size: 629 355 111 336
Fragments arranged by size:
629 355 336 111
SacI G_AGCT'C
Cuts at: 0 7 1431
Size: 7 1424
SanDI GG'GwC_CC
Cuts at: 0 611 1431
Size: 611 820
SfcI C'TryA_G
Cuts at: 0 157 534 587 1431
Size: 157 377 53 844
Fragments arranged by size:
844 377 157 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 233 1431
Size: 233 1198
SfoI GGC'GCC
Cuts at: 0 30 1380 1431
Size: 30 1350 51
Fragments arranged by size:
1350 51 30
SmaI CCC'GGG
Cuts at: 0 182 381 774 1431
Size: 182 199 393 657
Fragments arranged by size:
657 393 199 182
SmlI C'TyrA_G
Cuts at: 0 1138 1431
Size: 1138 293
StuI AGG'CCT
Cuts at: 0 227 1431
Size: 227 1204
StyI C'CwwG_G
Cuts at: 0 410 426 1331 1382 1431
Size: 410 16 905 51 49
Fragments arranged by size:
905 410 51 49 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 158 303 823 1431
Size: 158 145 520 608
Fragments arranged by size:
608 520 158 145
Tth111I GACn'n_nGTC
Cuts at: 0 859 898 1431
Size: 859 39 533
Fragments arranged by size:
859 533 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1338 1431
Size: 354 984 93
Fragments arranged by size:
984 354 93
XhoI C'TCGA_G
Cuts at: 0 1138 1431
Size: 1138 293
XmaI C'CCGG_G
Cuts at: 0 180 379 772 1431
Size: 180 199 393 659
Fragments arranged by size:
659 393 199 180
Enzymes that do cut:
AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NarI NcoI PflMI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BpuEI BsaBI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XmnI ZraI