Restriction map for
pFN21AE5626
(Linear) (Six-base) MAPSORT of: KIEE5626.seq Check: 5164 from: 1 to: 1449
KIEE5626.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 410 1449
Size: 410 1039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1449
Size: 1372 77
AfeI AGC'GCT
Cuts at: 0 664 721 1449
Size: 664 57 728
Fragments arranged by size:
728 664 57
AflIII A'CryG_T
Cuts at: 0 363 767 1449
Size: 363 404 682
Fragments arranged by size:
682 404 363
AleI CACnn'nnGTG
Cuts at: 0 79 1051 1449
Size: 79 972 398
Fragments arranged by size:
972 398 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 817 1449
Size: 817 632
AlwNI CAG_nnn'CTG
Cuts at: 0 189 342 546 594 789 1443 1449
Size: 189 153 204 48 195 654 6
Fragments arranged by size:
654 204 195 189 153 48 6
ApaLI G'TGCA_C
Cuts at: 0 1078 1449
Size: 1078 371
ApoI r'AATT_y
Cuts at: 0 808 1449
Size: 808 641
AvaI C'yCGr_G
Cuts at: 0 19 926 976 1449
Size: 19 907 50 473
Fragments arranged by size:
907 473 50 19
BamHI G'GATC_C
Cuts at: 0 198 899 1449
Size: 198 701 550
Fragments arranged by size:
701 550 198
BanI G'GyrC_C
Cuts at: 0 386 675 1449
Size: 386 289 774
Fragments arranged by size:
774 386 289
BanII G_rGCy'C
Cuts at: 0 20 350 983 1449
Size: 20 330 633 466
Fragments arranged by size:
633 466 330 20
BbeI G_GCGC'C
Cuts at: 0 390 1449
Size: 390 1059
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 1449
Size: 160 1289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 328 1449
Size: 294 34 1121
Fragments arranged by size:
1121 294 34
BglI GCCn_nnn'nGGC
Cuts at: 0 291 517 1449
Size: 291 226 932
Fragments arranged by size:
932 291 226
BglII A'GATC_T
Cuts at: 0 989 1449
Size: 989 460
BlpI GC'TnA_GC
Cuts at: 0 1292 1449
Size: 1292 157
Bme1580I G_kGCm'C
Cuts at: 0 1082 1449
Size: 1082 367
BmgBI CAC'GTC
Cuts at: 0 918 1449
Size: 918 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 1420 1449
Size: 1420 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1137 1449
Size: 1137 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 364 526 1132 1216 1273 1294 1449
Size: 130 234 162 606 84 57 21 155
Fragments arranged by size:
606 234 162 155 130 84 57 21
BsaAI yAC'GTr
Cuts at: 0 366 770 1449
Size: 366 404 679
Fragments arranged by size:
679 404 366
BsaBI GATnn'nnATC
Cuts at: 0 904 994 1449
Size: 904 90 455
Fragments arranged by size:
904 455 90
BsaHI Gr'CG_yC
Cuts at: 0 387 695 1389 1449
Size: 387 308 694 60
Fragments arranged by size:
694 387 308 60
BsaWI w'CCGG_w
Cuts at: 0 896 1449
Size: 896 553
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 907 937 1449
Size: 907 30 512
Fragments arranged by size:
907 512 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1105 1129 1357 1449
Size: 1105 24 228 92
Fragments arranged by size:
1105 228 92 24
BseYI C'CCAG_C
Cuts at: 0 57 1449
Size: 57 1392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 468 817 1043 1449
Size: 103 365 349 226 406
Fragments arranged by size:
406 365 349 226 103
BsiEI CG_ry'CG
Cuts at: 0 1011 1449
Size: 1011 438
BsiHKAI G_wGCw'C
Cuts at: 0 20 350 643 737 1082 1449
Size: 20 330 293 94 345 367
Fragments arranged by size:
367 345 330 293 94 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1339 1449
Size: 1339 110
Bsp1286I G_dGCh'C
Cuts at: 0 20 350 643 737 983 1082 1449
Size: 20 330 293 94 246 99 367
Fragments arranged by size:
367 330 293 246 99 94 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 677 787 1449
Size: 410 267 110 662
Fragments arranged by size:
662 410 267 110
BsrFI r'CCGG_y
Cuts at: 0 464 528 1319 1449
Size: 464 64 791 130
Fragments arranged by size:
791 464 130 64
BssSI C'ACGA_G
Cuts at: 0 37 736 1449
Size: 37 699 713
Fragments arranged by size:
713 699 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 215 546 779 1449
Size: 215 331 233 670
Fragments arranged by size:
670 331 233 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 1050 1449
Size: 243 807 399
Fragments arranged by size:
807 399 243
BstYI r'GATC_y
Cuts at: 0 198 434 899 989 1148 1449
Size: 198 236 465 90 159 301
Fragments arranged by size:
465 301 236 198 159 90
BtgI C'CryG_G
Cuts at: 0 497 644 824 1124 1449
Size: 497 147 180 300 325
Fragments arranged by size:
497 325 300 180 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 1449
Size: 357 1092
BtsI GCAGTG_nn'
Cuts at: 0 153 544 1449
Size: 153 391 905
Fragments arranged by size:
905 391 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 899 934 1449
Size: 899 35 515
Fragments arranged by size:
899 515 35
DraIII CAC_nnn'GTG
Cuts at: 0 1077 1449
Size: 1077 372
EaeI y'GGCC_r
Cuts at: 0 596 1008 1298 1377 1449
Size: 596 412 290 79 72
Fragments arranged by size:
596 412 290 79 72
EagI C'GGCC_G
Cuts at: 0 1008 1449
Size: 1008 441
EarI CTCTTCn'nnn_
Cuts at: 0 1124 1170 1449
Size: 1124 46 279
Fragments arranged by size:
1124 279 46
EcoICRI GAG'CTC
Cuts at: 0 18 348 1449
Size: 18 330 1101
Fragments arranged by size:
1101 330 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 364 526 1132 1216 1273 1294 1372
Size: 130 234 162 606 84 57 21 78
Cuts at: 1372 1449
Size: 77
Fragments arranged by size:
606 234 162 130 84 78 77 57
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1156 1449
Size: 1156 293
EcoO109I rG'GnC_Cy
Cuts at: 0 273 630 930 1263 1412 1440 1449
Size: 273 357 300 333 149 28 9
Fragments arranged by size:
357 333 300 273 149 28 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 1449
Size: 456 993
FspI TGC'GCA
Cuts at: 0 406 805 1449
Size: 406 399 644
Fragments arranged by size:
644 406 399
HaeII r_GCGC'y
Cuts at: 0 343 390 560 666 723 1326 1449
Size: 343 47 170 106 57 603 123
Fragments arranged by size:
603 343 170 123 106 57 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 907 939 1449
Size: 730 32 145 32 510
Fragments arranged by size:
730 510 145 32 32
HincII GTy'rAC
Cuts at: 0 1164 1449
Size: 1164 285
KasI G'GCGC_C
Cuts at: 0 386 1449
Size: 386 1063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1449
Size: 437 1012
MscI TGG'CCA
Cuts at: 0 598 1300 1379 1449
Size: 598 702 79 70
Fragments arranged by size:
702 598 79 70
MslI CAynn'nnrTG
Cuts at: 0 79 325 1051 1449
Size: 79 246 726 398
Fragments arranged by size:
726 398 246 79
MspA1I CmG'CkG
Cuts at: 0 57 654 1245 1449
Size: 57 597 591 204
Fragments arranged by size:
597 591 204 57
NaeI GCC'GGC
Cuts at: 0 530 1449
Size: 530 919
NarI GG'CG_CC
Cuts at: 0 387 1449
Size: 387 1062
NgoMIV G'CCGG_C
Cuts at: 0 528 1449
Size: 528 921
PasI CC'CwG_GG
Cuts at: 0 1437 1449
Size: 1437 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 1443 1449
Size: 342 1101 6
Fragments arranged by size:
1101 342 6
PfoI T'CCnGG_A
Cuts at: 0 107 578 725 1250 1449
Size: 107 471 147 525 199
Fragments arranged by size:
525 471 199 147 107
PmlI CAC'GTG
Cuts at: 0 366 770 1449
Size: 366 404 679
Fragments arranged by size:
679 404 366
PpuMI rG'GwC_Cy
Cuts at: 0 273 1263 1412 1449
Size: 273 990 149 37
Fragments arranged by size:
990 273 149 37
PstI C_TGCA'G
Cuts at: 0 590 1449
Size: 590 859
PvuII CAG'CTG
Cuts at: 0 57 654 1245 1449
Size: 57 597 591 204
Fragments arranged by size:
597 591 204 57
SacI G_AGCT'C
Cuts at: 0 20 350 1449
Size: 20 330 1099
Fragments arranged by size:
1099 330 20
SanDI GG'GwC_CC
Cuts at: 0 1412 1449
Size: 1412 37
SexAI A'CCwGG_T
Cuts at: 0 701 1073 1449
Size: 701 372 376
Fragments arranged by size:
701 376 372
SfcI C'TryA_G
Cuts at: 0 586 1144 1449
Size: 586 558 305
Fragments arranged by size:
586 558 305
SfoI GGC'GCC
Cuts at: 0 388 1449
Size: 388 1061
SmaI CCC'GGG
Cuts at: 0 928 978 1449
Size: 928 50 471
Fragments arranged by size:
928 471 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 246 583 1410 1449
Size: 246 337 827 39
Fragments arranged by size:
827 337 246 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1005 1037 1449
Size: 1005 32 412
Fragments arranged by size:
1005 412 32
Tth111I GACn'n_nGTC
Cuts at: 0 1404 1449
Size: 1404 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 1449
Size: 240 1209
XmaI C'CCGG_G
Cuts at: 0 926 976 1449
Size: 926 50 473
Fragments arranged by size:
926 473 50
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV PasI PflMI PfoI PmlI PpuMI
PstI PvuII SacI SanDI SexAI SfcI SfoI SmaI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BfrBI BmtI Bpu10I BpuEI BsaI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI DraI DrdI EciI EcoRI EcoRV
FseI FspAI HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI XbaI XhoI XmnI ZraI