(Linear) (Six-base) MAPSORT of: KIEE5623.seq  Check: 3177  from: 1  to: 1422



KIEE5623.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     791    1422 
   Size:        791     631
 
AfeI AGC'GCT

Cuts at:      0     130    1422 
   Size:        130    1292
 
ApoI r'AATT_y

Cuts at:      0     415     685    1422 
   Size:        415     270     737

  Fragments arranged by size: 

                 737     415     270
 
AvaI C'yCGr_G

Cuts at:      0      42    1422 
   Size:         42    1380
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     370     403    1422 
   Size:        370      33    1019

  Fragments arranged by size: 

                1019     370      33
 
BbvCI CC'TCA_GC

Cuts at:      0     121    1422 
   Size:        121    1301
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     189     223    1422 
   Size:        189      34    1199

  Fragments arranged by size: 

                1199     189      34
 
BclI T'GATC_A

Cuts at:      0      92    1422 
   Size:         92    1330
 
BfrBI ATG'CAT

Cuts at:      0     258    1422 
   Size:        258    1164
 
BglI GCCn_nnn'nGGC

Cuts at:      0     241    1422 
   Size:        241    1181
 
BglII A'GATC_T

Cuts at:      0     402     981    1422 
   Size:        402     579     441

  Fragments arranged by size: 

                 579     441     402
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     150    1422 
   Size:        150    1272
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491    1422 
   Size:        491     931
 
Bpu10I CC'TnA_GC

Cuts at:      0     121    1422 
   Size:        121    1301
 
BsaAI yAC'GTr

Cuts at:      0     495    1422 
   Size:        495     927
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265    1422 
   Size:        265    1157
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1001    1422 
   Size:       1001     421
 
BsiWI C'GTAC_G

Cuts at:      0     495    1422 
   Size:        495     927
 
BsrBI CCG'CTC

Cuts at:      0      26    1422 
   Size:         26    1396
 
BsrDI GCAATG_nn'

Cuts at:      0     200     305    1322    1422 
   Size:        200     105    1017     100

  Fragments arranged by size: 

                1017     200     105     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1024    1422 
   Size:       1024     398
 
BstEII G'GTnAC_C

Cuts at:      0    1240    1422 
   Size:       1240     182
 
BstYI r'GATC_y

Cuts at:      0     402     834     981    1422 
   Size:        402     432     147     441

  Fragments arranged by size: 

                 441     432     402     147
 
BtgI C'CryG_G

Cuts at:      0     245    1422 
   Size:        245    1177
 
EaeI y'GGCC_r

Cuts at:      0     261    1422 
   Size:        261    1161
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28    1422 
   Size:         28    1394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     791    1422 
   Size:        491     300     631

  Fragments arranged by size: 

                 631     491     300
 
EcoRV GAT'ATC

Cuts at:      0     435    1422 
   Size:        435     987
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     779     796    1189    1276    1422 
   Size:        779      17     393      87     146

  Fragments arranged by size: 

                 779     393     146      87      17
 
HaeII r_GCGC'y

Cuts at:      0     132    1422 
   Size:        132    1290
 
HindIII A'AGCT_T

Cuts at:      0     632    1422 
   Size:        632     790
 
MscI TGG'CCA

Cuts at:      0     263    1422 
   Size:        263    1159
 
MslI CAynn'nnrTG

Cuts at:      0     163     338    1422 
   Size:        163     175    1084

  Fragments arranged by size: 

                1084     175     163
 
NcoI C'CATG_G

Cuts at:      0     245    1422 
   Size:        245    1177
 
NsiI A_TGCA'T

Cuts at:      0     260    1422 
   Size:        260    1162
 
PfoI T'CCnGG_A

Cuts at:      0     152    1422 
   Size:        152    1270
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     743     775    1422 
   Size:        743      32     647

  Fragments arranged by size: 

                 743     647      32
 
PstI C_TGCA'G

Cuts at:      0     241    1422 
   Size:        241    1181
 
SbfI CC_TGCA'GG

Cuts at:      0     241    1422 
   Size:        241    1181
 
SfcI C'TryA_G

Cuts at:      0     237    1422 
   Size:        237    1185
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     241    1422 
   Size:        241    1181
 
SmaI CCC'GGG

Cuts at:      0      44    1422 
   Size:         44    1378
 
SnaBI TAC'GTA

Cuts at:      0     495    1422 
   Size:        495     927
 
SrfI GCCC'GGGC

Cuts at:      0      44    1422 
   Size:         44    1378
 
SspI AAT'ATT

Cuts at:      0     817    1422 
   Size:        817     605
 
StuI AGG'CCT

Cuts at:      0     235    1422 
   Size:        235    1187
 
StyI C'CwwG_G

Cuts at:      0     185     194     245    1422 
   Size:        185       9      51    1177

  Fragments arranged by size: 

                1177     185      51       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153    1422 
   Size:        153    1269
 
TatI w'GTAC_w

Cuts at:      0     101    1119    1422 
   Size:        101    1018     303

  Fragments arranged by size: 

                1018     303     101
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1262    1294    1422 
   Size:       1262      32     128

  Fragments arranged by size: 

                1262     128      32
 
XmaI C'CCGG_G

Cuts at:      0      42    1422 
   Size:         42    1380
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     AvaI     BaeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BplI     BpmI   Bpu10I    BsaAI    BseRI
     BsgI    BsiWI    BsrBI    BsrDI   BstAPI   BstEII    BstYI     BtgI
     EaeI     EciI  Eco57MI    EcoRV     FalI    HaeII  HindIII     MscI
     MslI     NcoI     NsiI     PfoI     PsrI     PstI     SbfI     SfcI
     SfiI     SmaI    SnaBI     SrfI     SspI     StuI     StyI    TaqII
     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BlpI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII
    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI
     SpeI     SphI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI