Restriction map for
pFN21AE5618
(Linear) (Six-base) MAPSORT of: KIEE5618.seq Check: 1589 from: 1 to: 1599
KIEE5618.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1054 1599
Size: 1054 545
AatII G_ACGT'C
Cuts at: 0 10 1599
Size: 10 1589
Acc65I G'GTAC_C
Cuts at: 0 999 1599
Size: 999 600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 290 1526 1599
Size: 137 153 1236 73
Fragments arranged by size:
1236 153 137 73
AflIII A'CryG_T
Cuts at: 0 1588 1599
Size: 1588 11
AlwNI CAG_nnn'CTG
Cuts at: 0 250 1599
Size: 250 1349
ApaLI G'TGCA_C
Cuts at: 0 1067 1599
Size: 1067 532
ApoI r'AATT_y
Cuts at: 0 208 644 843 1031 1599
Size: 208 436 199 188 568
Fragments arranged by size:
568 436 208 199 188
AseI AT'TA_AT
Cuts at: 0 1075 1599
Size: 1075 524
AvaI C'yCGr_G
Cuts at: 0 1388 1599
Size: 1388 211
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1322 1355 1599
Size: 1322 33 244
Fragments arranged by size:
1322 244 33
BanI G'GyrC_C
Cuts at: 0 999 1599
Size: 999 600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 1599
Size: 28 1571
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 1599
Size: 282 1317
BglII A'GATC_T
Cuts at: 0 465 1599
Size: 465 1134
Bme1580I G_kGCm'C
Cuts at: 0 917 1071 1599
Size: 917 154 528
Fragments arranged by size:
917 528 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 849 1599
Size: 188 661 750
Fragments arranged by size:
750 661 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1599
Size: 171 1428
BsaI GGTCTCn'nnnn_
Cuts at: 0 395 938 1599
Size: 395 543 661
Fragments arranged by size:
661 543 395
BsaAI yAC'GTr
Cuts at: 0 1521 1599
Size: 1521 78
BsaHI Gr'CG_yC
Cuts at: 0 7 1599
Size: 7 1592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 954 984 1599
Size: 954 30 615
Fragments arranged by size:
954 615 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1373 1376 1599
Size: 1373 3 223
Fragments arranged by size:
1373 223 3
BseYI C'CCAG_C
Cuts at: 0 365 1206 1599
Size: 365 841 393
Fragments arranged by size:
841 393 365
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1599
Size: 1421 178
BsiHKAI G_wGCw'C
Cuts at: 0 1071 1305 1599
Size: 1071 234 294
Fragments arranged by size:
1071 294 234
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 1599
Size: 14 1585
Bsp1286I G_dGCh'C
Cuts at: 0 917 1071 1305 1599
Size: 917 154 234 294
Fragments arranged by size:
917 294 234 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 987 1054 1599
Size: 154 833 67 545
Fragments arranged by size:
833 545 154 67
BsrDI GCAATG_nn'
Cuts at: 0 998 1599
Size: 998 601
BsrGI T'GTAC_A
Cuts at: 0 91 1599
Size: 91 1508
BstYI r'GATC_y
Cuts at: 0 39 465 1599
Size: 39 426 1134
Fragments arranged by size:
1134 426 39
BtgI C'CryG_G
Cuts at: 0 56 284 608 1326 1599
Size: 56 228 324 718 273
Fragments arranged by size:
718 324 273 228 56
BtsI GCAGTG_nn'
Cuts at: 0 840 944 1442 1599
Size: 840 104 498 157
Fragments arranged by size:
840 498 157 104
DraIII CAC_nnn'GTG
Cuts at: 0 1524 1599
Size: 1524 75
EaeI y'GGCC_r
Cuts at: 0 348 1374 1599
Size: 348 1026 225
Fragments arranged by size:
1026 348 225
EarI CTCTTCn'nnn_
Cuts at: 0 266 1599
Size: 266 1333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 188 290 849 1526 1599
Size: 137 51 102 559 677 73
Fragments arranged by size:
677 559 137 102 73 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 150 398 1599
Size: 150 248 1201
Fragments arranged by size:
1201 248 150
EcoO109I rG'GnC_Cy
Cuts at: 0 22 1599
Size: 22 1577
EcoRI G'AATT_C
Cuts at: 0 208 644 1599
Size: 208 436 955
Fragments arranged by size:
955 436 208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 142 1599
Size: 142 1457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 910 942 1157 1189 1371 1403
Size: 757 32 121 32 215 32 182 32
Cuts at: 1403 1411 1443 1599
Size: 8 32 156
Fragments arranged by size:
757 215 182 156 121 32 32 32
32 32 8
HincII GTy'rAC
Cuts at: 0 266 1231 1599
Size: 266 965 368
Fragments arranged by size:
965 368 266
HindIII A'AGCT_T
Cuts at: 0 562 1599
Size: 562 1037
KpnI G_GTAC'C
Cuts at: 0 1003 1599
Size: 1003 596
MfeI C'AATT_G
Cuts at: 0 104 1599
Size: 104 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1117 1260 1599
Size: 328 789 143 339
Fragments arranged by size:
789 339 328 143
MscI TGG'CCA
Cuts at: 0 1376 1599
Size: 1376 223
MspA1I CmG'CkG
Cuts at: 0 36 1599
Size: 36 1563
NcoI C'CATG_G
Cuts at: 0 284 608 1326 1599
Size: 284 324 718 273
Fragments arranged by size:
718 324 284 273
NdeI CA'TA_TG
Cuts at: 0 131 1599
Size: 131 1468
NspI r_CATG'y
Cuts at: 0 1592 1599
Size: 1592 7
PasI CC'CwG_GG
Cuts at: 0 910 1599
Size: 910 689
PciI A'CATG_T
Cuts at: 0 1588 1599
Size: 1588 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 1498 1524 1599
Size: 1498 26 75
Fragments arranged by size:
1498 75 26
PpuMI rG'GwC_Cy
Cuts at: 0 22 1599
Size: 22 1577
PspXI vC'TCGA_Gb
Cuts at: 0 1388 1599
Size: 1388 211
PstI C_TGCA'G
Cuts at: 0 801 1238 1599
Size: 801 437 361
Fragments arranged by size:
801 437 361
PvuII CAG'CTG
Cuts at: 0 36 1599
Size: 36 1563
SanDI GG'GwC_CC
Cuts at: 0 22 1599
Size: 22 1577
SfcI C'TryA_G
Cuts at: 0 251 797 1234 1599
Size: 251 546 437 365
Fragments arranged by size:
546 437 365 251
SmlI C'TyrA_G
Cuts at: 0 150 1388 1599
Size: 150 1238 211
Fragments arranged by size:
1238 211 150
SpeI A'CTAG_T
Cuts at: 0 1087 1581 1599
Size: 1087 494 18
Fragments arranged by size:
1087 494 18
StyI C'CwwG_G
Cuts at: 0 284 608 973 1326 1599
Size: 284 324 365 353 273
Fragments arranged by size:
365 353 324 284 273
TatI w'GTAC_w
Cuts at: 0 91 1599
Size: 91 1508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 969 1001 1599
Size: 969 32 598
Fragments arranged by size:
969 598 32
Tth111I GACn'n_nGTC
Cuts at: 0 1242 1599
Size: 1242 357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 1599
Size: 33 1566
XhoI C'TCGA_G
Cuts at: 0 1388 1599
Size: 1388 211
XmnI GAAnn'nnTTC
Cuts at: 0 843 1599
Size: 843 756
ZraI GAC'GTC
Cuts at: 0 8 1599
Size: 8 1591
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI ApaLI ApoI
AseI AvaI BaeI BanI BcgI BciVI BglII Bme1580I
BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BstYI
BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII KpnI MfeI MmeI
MscI MspA1I NcoI NdeI NspI PasI PciI PflMI
PpuMI PspXI PstI PvuII SanDI SfcI SmlI SpeI
StyI TatI TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvrII BamHI BanII BbeI
BbsI BbvCI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HaeII HpaI KasI MluI MslI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TaqII XbaI XmaI