Restriction map for
pFN21AE5615
(Linear) (Six-base) MAPSORT of: KIEE5615.seq Check: 6766 from: 1 to: 1713
KIEE5615.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AccI GT'mk_AC
Cuts at: 0 826 1223 1713
Size: 826 397 490
Fragments arranged by size:
826 490 397
Acc65I G'GTAC_C
Cuts at: 0 206 1713
Size: 206 1507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1713
Size: 878 835
AflII C'TTAA_G
Cuts at: 0 1432 1713
Size: 1432 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 890 922 1713
Size: 890 32 791
Fragments arranged by size:
890 791 32
ApaLI G'TGCA_C
Cuts at: 0 607 1713
Size: 607 1106
ApoI r'AATT_y
Cuts at: 0 74 196 421 886 1255 1273 1330 1713
Size: 74 122 225 465 369 18 57 383
Fragments arranged by size:
465 383 369 225 122 74 57 18
AseI AT'TA_AT
Cuts at: 0 689 1713
Size: 689 1024
AvaI C'yCGr_G
Cuts at: 0 299 1713
Size: 299 1414
BanI G'GyrC_C
Cuts at: 0 206 1713
Size: 206 1507
BbsI GAAGACnn'nnnn_
Cuts at: 0 590 995 1713
Size: 590 405 718
Fragments arranged by size:
718 590 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 910 944 1713
Size: 910 34 769
Fragments arranged by size:
910 769 34
Bme1580I G_kGCm'C
Cuts at: 0 611 1713
Size: 611 1102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1585 1713
Size: 1585 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 419 1163 1713
Size: 41 378 744 550
Fragments arranged by size:
744 550 378 41
Bpu10I CC'TnA_GC
Cuts at: 0 1626 1713
Size: 1626 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 302 1192 1713
Size: 302 890 521
Fragments arranged by size:
890 521 302
BsaBI GATnn'nnATC
Cuts at: 0 13 1713
Size: 13 1700
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 1713
Size: 1534 30 149
Fragments arranged by size:
1534 149 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1269 1673 1713
Size: 516 753 404 40
Fragments arranged by size:
753 516 404 40
BsiHKAI G_wGCw'C
Cuts at: 0 611 1713
Size: 611 1102
BsiWI C'GTAC_G
Cuts at: 0 839 1713
Size: 839 874
BsmI GAATG_Cn'
Cuts at: 0 803 1713
Size: 803 910
Bsp1286I G_dGCh'C
Cuts at: 0 611 1713
Size: 611 1102
BspHI T'CATG_A
Cuts at: 0 395 1181 1713
Size: 395 786 532
Fragments arranged by size:
786 532 395
BsrDI GCAATG_nn'
Cuts at: 0 972 1702 1713
Size: 972 730 11
Fragments arranged by size:
972 730 11
BssSI C'ACGA_G
Cuts at: 0 215 1713
Size: 215 1498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1114 1713
Size: 1114 599
BtgI C'CryG_G
Cuts at: 0 493 1713
Size: 493 1220
BtsI GCAGTG_nn'
Cuts at: 0 297 1713
Size: 297 1416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 933 968 1713
Size: 933 35 745
Fragments arranged by size:
933 745 35
EaeI y'GGCC_r
Cuts at: 0 319 941 1713
Size: 319 622 772
Fragments arranged by size:
772 622 319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 419 878 1163 1713
Size: 41 378 459 285 550
Fragments arranged by size:
550 459 378 285 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1625 1713
Size: 1625 88
EcoO109I rG'GnC_Cy
Cuts at: 0 1577 1713
Size: 1577 136
EcoRI G'AATT_C
Cuts at: 0 74 1255 1273 1713
Size: 74 1181 18 440
Fragments arranged by size:
1181 440 74 18
EcoRV GAT'ATC
Cuts at: 0 357 600 1713
Size: 357 243 1113
Fragments arranged by size:
1113 357 243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1539 1713
Size: 1539 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1154 1186 1713
Size: 1154 32 527
Fragments arranged by size:
1154 527 32
HincII GTy'rAC
Cuts at: 0 750 827 1500 1713
Size: 750 77 673 213
Fragments arranged by size:
750 673 213 77
HindIII A'AGCT_T
Cuts at: 0 1509 1713
Size: 1509 204
HpaI GTT'AAC
Cuts at: 0 750 1713
Size: 750 963
KpnI G_GTAC'C
Cuts at: 0 210 1713
Size: 210 1503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1713
Size: 332 1381
MscI TGG'CCA
Cuts at: 0 321 943 1713
Size: 321 622 770
Fragments arranged by size:
770 622 321
MspA1I CmG'CkG
Cuts at: 0 799 1713
Size: 799 914
NdeI CA'TA_TG
Cuts at: 0 1674 1713
Size: 1674 39
NspI r_CATG'y
Cuts at: 0 490 925 1713
Size: 490 435 788
Fragments arranged by size:
788 490 435
PflMI CCAn_nnn'nTGG
Cuts at: 0 102 1675 1713
Size: 102 1573 38
Fragments arranged by size:
1573 102 38
PfoI T'CCnGG_A
Cuts at: 0 756 1713
Size: 756 957
PpuMI rG'GwC_Cy
Cuts at: 0 1577 1713
Size: 1577 136
PshAI GACnn'nnGTC
Cuts at: 0 1157 1713
Size: 1157 556
PstI C_TGCA'G
Cuts at: 0 483 1713
Size: 483 1230
SalI G'TCGA_C
Cuts at: 0 825 1713
Size: 825 888
SfcI C'TryA_G
Cuts at: 0 236 479 1572 1713
Size: 236 243 1093 141
Fragments arranged by size:
1093 243 236 141
SmlI C'TyrA_G
Cuts at: 0 299 1432 1713
Size: 299 1133 281
Fragments arranged by size:
1133 299 281
SspI AAT'ATT
Cuts at: 0 998 1713
Size: 998 715
StyI C'CwwG_G
Cuts at: 0 322 699 1557 1713
Size: 322 377 858 156
Fragments arranged by size:
858 377 322 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1409 1713
Size: 610 799 304
Fragments arranged by size:
799 610 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1534 1566 1713
Size: 1534 32 147
Fragments arranged by size:
1534 147 32
XhoI C'TCGA_G
Cuts at: 0 299 1713
Size: 299 1414
XmnI GAAnn'nnTTC
Cuts at: 0 587 1713
Size: 587 1126
Enzymes that do cut:
AccI Acc65I AcuI AflII AloI ApaLI ApoI AseI
AvaI BanI BbsI BcgI Bme1580I BplI BpmI Bpu10I
BsaI BsaBI BsaXI BsgI BsiHKAI BsiWI BsmI Bsp1286I
BspHI BsrDI BssSI BstXI BtgI BtsI CspCI EaeI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI KpnI MmeI MscI MspA1I NdeI NspI
PflMI PfoI PpuMI PshAI PstI SalI SfcI SmlI
SspI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsiEI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EarI EciI
EcoICRI FseI FspI FspAI HaeII KasI MfeI MluI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TatI Tth111I XbaI XcmI XmaI ZraI