Restriction map for
pFN21AE5614
(Linear) (Six-base) MAPSORT of: KIEE5614.seq Check: 834 from: 1 to: 1593
KIEE5614.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AatII G_ACGT'C
Cuts at: 0 758 1593
Size: 758 835
Acc65I G'GTAC_C
Cuts at: 0 1325 1593
Size: 1325 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 613 986 1258 1593
Size: 517 96 373 272 335
Fragments arranged by size:
517 373 335 272 96
AflIII A'CryG_T
Cuts at: 0 1319 1593
Size: 1319 274
AleI CACnn'nnGTG
Cuts at: 0 1525 1593
Size: 1525 68
AlwNI CAG_nnn'CTG
Cuts at: 0 1169 1593
Size: 1169 424
ApaLI G'TGCA_C
Cuts at: 0 580 855 1593
Size: 580 275 738
Fragments arranged by size:
738 580 275
ApoI r'AATT_y
Cuts at: 0 390 1202 1593
Size: 390 812 391
Fragments arranged by size:
812 391 390
AvaI C'yCGr_G
Cuts at: 0 1158 1593
Size: 1158 435
BanI G'GyrC_C
Cuts at: 0 169 1325 1593
Size: 169 1156 268
Fragments arranged by size:
1156 268 169
BbvCI CC'TCA_GC
Cuts at: 0 1300 1593
Size: 1300 293
BciVI GTATCCnnnnn_n'
Cuts at: 0 864 1479 1593
Size: 864 615 114
Fragments arranged by size:
864 615 114
BclI T'GATC_A
Cuts at: 0 36 1593
Size: 36 1557
BfrBI ATG'CAT
Cuts at: 0 123 165 453 1593
Size: 123 42 288 1140
Fragments arranged by size:
1140 288 123 42
BglI GCCn_nnn'nGGC
Cuts at: 0 929 1593
Size: 929 664
Bme1580I G_kGCm'C
Cuts at: 0 174 584 859 1532 1593
Size: 174 410 275 673 61
Fragments arranged by size:
673 410 275 174 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 421 844 1593
Size: 421 423 749
Fragments arranged by size:
749 423 421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 272 861 1094 1527 1593
Size: 170 102 589 233 433 66
Fragments arranged by size:
589 433 233 170 102 66
Bpu10I CC'TnA_GC
Cuts at: 0 1126 1300 1593
Size: 1126 174 293
Fragments arranged by size:
1126 293 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1593
Size: 1192 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1593
Size: 1093 500
BsaBI GATnn'nnATC
Cuts at: 0 544 1593
Size: 544 1049
BsaHI Gr'CG_yC
Cuts at: 0 755 1593
Size: 755 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 874 904 1073 1103 1288 1318
Size: 580 30 264 30 169 30 185 30
Cuts at: 1318 1593
Size: 275
Fragments arranged by size:
580 275 264 185 169 30 30 30
30
BsiHKAI G_wGCw'C
Cuts at: 0 584 859 1593
Size: 584 275 734
Fragments arranged by size:
734 584 275
BsmI GAATG_Cn'
Cuts at: 0 123 412 480 1130 1593
Size: 123 289 68 650 463
Fragments arranged by size:
650 463 289 123 68
Bsp1286I G_dGCh'C
Cuts at: 0 174 584 859 1532 1593
Size: 174 410 275 673 61
Fragments arranged by size:
673 410 275 174 61
BsrDI GCAATG_nn'
Cuts at: 0 167 1424 1593
Size: 167 1257 169
Fragments arranged by size:
1257 169 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1044 1169 1581 1593
Size: 1044 125 412 12
Fragments arranged by size:
1044 412 125 12
BstEII G'GTnAC_C
Cuts at: 0 980 1593
Size: 980 613
BstYI r'GATC_y
Cuts at: 0 1009 1593
Size: 1009 584
Bsu36I CC'TnA_GG
Cuts at: 0 327 1145 1593
Size: 327 818 448
Fragments arranged by size:
818 448 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 1593
Size: 468 1125
BtsI GCAGTG_nn'
Cuts at: 0 4 417 1593
Size: 4 413 1176
Fragments arranged by size:
1176 413 4
DraIII CAC_nnn'GTG
Cuts at: 0 579 1593
Size: 579 1014
EaeI y'GGCC_r
Cuts at: 0 623 930 1593
Size: 623 307 663
Fragments arranged by size:
663 623 307
EarI CTCTTCn'nnn_
Cuts at: 0 48 758 1593
Size: 48 710 835
Fragments arranged by size:
835 710 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 272 517 613 861 986 1094 1258
Size: 170 102 245 96 248 125 108 164
Cuts at: 1258 1527 1593
Size: 269 66
Fragments arranged by size:
269 248 245 170 164 125 108 102
96 66
EcoO109I rG'GnC_Cy
Cuts at: 0 323 1212 1502 1593
Size: 323 889 290 91
Fragments arranged by size:
889 323 290 91
EcoRV GAT'ATC
Cuts at: 0 542 666 1593
Size: 542 124 927
Fragments arranged by size:
927 542 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1017 1593
Size: 1017 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1073 1105 1288 1320 1593
Size: 580 32 461 32 183 32 273
Fragments arranged by size:
580 461 273 183 32 32 32
HincII GTy'rAC
Cuts at: 0 264 555 1359 1593
Size: 264 291 804 234
Fragments arranged by size:
804 291 264 234
HindIII A'AGCT_T
Cuts at: 0 999 1593
Size: 999 594
HpaI GTT'AAC
Cuts at: 0 264 1593
Size: 264 1329
KpnI G_GTAC'C
Cuts at: 0 1329 1593
Size: 1329 264
MscI TGG'CCA
Cuts at: 0 625 932 1593
Size: 625 307 661
Fragments arranged by size:
661 625 307
MslI CAynn'nnrTG
Cuts at: 0 1525 1593
Size: 1525 68
NsiI A_TGCA'T
Cuts at: 0 125 167 455 1593
Size: 125 42 288 1138
Fragments arranged by size:
1138 288 125 42
NspI r_CATG'y
Cuts at: 0 123 315 453 457 1323 1593
Size: 123 192 138 4 866 270
Fragments arranged by size:
866 270 192 138 123 4
PciI A'CATG_T
Cuts at: 0 1319 1593
Size: 1319 274
PfoI T'CCnGG_A
Cuts at: 0 896 1593
Size: 896 697
PpuMI rG'GwC_Cy
Cuts at: 0 323 1212 1502 1593
Size: 323 889 290 91
Fragments arranged by size:
889 323 290 91
PstI C_TGCA'G
Cuts at: 0 792 1049 1593
Size: 792 257 544
Fragments arranged by size:
792 544 257
SanDI GG'GwC_CC
Cuts at: 0 1502 1593
Size: 1502 91
SapI GCTCTTCn'nnn_
Cuts at: 0 48 1593
Size: 48 1545
SfcI C'TryA_G
Cuts at: 0 776 788 1045 1593
Size: 776 12 257 548
Fragments arranged by size:
776 548 257 12
SmlI C'TyrA_G
Cuts at: 0 1207 1593
Size: 1207 386
SphI G_CATG'C
Cuts at: 0 123 457 1593
Size: 123 334 1136
Fragments arranged by size:
1136 334 123
StuI AGG'CCT
Cuts at: 0 1114 1593
Size: 1114 479
StyI C'CwwG_G
Cuts at: 0 1497 1593
Size: 1497 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 957 1593
Size: 957 636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1494 1526 1593
Size: 1494 32 67
Fragments arranged by size:
1494 67 32
Tth111I GACn'n_nGTC
Cuts at: 0 1085 1593
Size: 1085 508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 1593
Size: 465 1128
ZraI GAC'GTC
Cuts at: 0 756 1593
Size: 756 837
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AlwNI ApaLI ApoI
AvaI BanI BbvCI BciVI BclI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstEII BstYI Bsu36I
BtgZI BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRV
FalI Hin4I HincII HindIII HpaI KpnI MscI MslI
NsiI NspI PciI PfoI PpuMI PstI SanDI SapI
SfcI SmlI SphI StuI StyI TaqII TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbsI BcgI BglII BlpI BmgBI BmtI
BplI BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstZ17I BtgI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI
FspAI HaeII KasI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TatI XbaI XhoI
XmaI XmnI