Restriction map for
pFN21AE5613
(Linear) (Six-base) MAPSORT of: KIEE5613.seq Check: 9011 from: 1 to: 1455
KIEE5613.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 955 1455
Size: 955 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 1444 1455
Size: 705 739 11
Fragments arranged by size:
739 705 11
AleI CACnn'nnGTG
Cuts at: 0 925 1455
Size: 925 530
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1455
Size: 48 1407
ApaI G_GGCC'C
Cuts at: 0 86 1455
Size: 86 1369
ApaLI G'TGCA_C
Cuts at: 0 486 1455
Size: 486 969
AscI GG'CGCG_CC
Cuts at: 0 25 1455
Size: 25 1430
AvaI C'yCGr_G
Cuts at: 0 85 94 783 1189 1440 1455
Size: 85 9 689 406 251 15
Fragments arranged by size:
689 406 251 85 15 9
BanI G'GyrC_C
Cuts at: 0 161 841 916 989 1455
Size: 161 680 75 73 466
Fragments arranged by size:
680 466 161 75 73
BanII G_rGCy'C
Cuts at: 0 86 92 221 1048 1455
Size: 86 6 129 827 407
Fragments arranged by size:
827 407 129 86 6
BbeI G_GCGC'C
Cuts at: 0 845 1455
Size: 845 610
BbsI GAAGACnn'nnnn_
Cuts at: 0 334 1455
Size: 334 1121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1308 1342 1455
Size: 1308 34 113
Fragments arranged by size:
1308 113 34
BglI GCCn_nnn'nGGC
Cuts at: 0 689 1375 1455
Size: 689 686 80
Fragments arranged by size:
689 686 80
Bme1580I G_kGCm'C
Cuts at: 0 86 490 919 977 992 1031 1455
Size: 86 404 429 58 15 39 424
Fragments arranged by size:
429 424 404 86 58 39 15
BmgBI CAC'GTC
Cuts at: 0 447 1455
Size: 447 1008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 1455
Size: 597 858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1455
Size: 166 1289
BsaHI Gr'CG_yC
Cuts at: 0 128 700 842 1455
Size: 128 572 142 613
Fragments arranged by size:
613 572 142 128
BsaWI w'CCGG_w
Cuts at: 0 398 1081 1455
Size: 398 683 374
Fragments arranged by size:
683 398 374
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 534 600 1455
Size: 454 80 66 855
Fragments arranged by size:
855 454 80 66
BseYI C'CCAG_C
Cuts at: 0 687 912 1035 1148 1455
Size: 687 225 123 113 307
Fragments arranged by size:
687 307 225 123 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 880 1455
Size: 301 579 575
Fragments arranged by size:
579 575 301
BsiEI CG_ry'CG
Cuts at: 0 207 1455
Size: 207 1248
BsiHKAI G_wGCw'C
Cuts at: 0 221 230 490 497 1455
Size: 221 9 260 7 958
Fragments arranged by size:
958 260 221 9 7
Bsp1286I G_dGCh'C
Cuts at: 0 86 92 221 230 490 497 919 977
Size: 86 6 129 9 260 7 422 58
Cuts at: 977 992 1031 1048 1455
Size: 15 39 17 407
Fragments arranged by size:
422 407 260 129 86 58 39 17
15 9 7 6
BspEI T'CCGG_A
Cuts at: 0 1081 1455
Size: 1081 374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 955 1455
Size: 955 500
BsrBI CCG'CTC
Cuts at: 0 189 651 1455
Size: 189 462 804
Fragments arranged by size:
804 462 189
BsrDI GCAATG_nn'
Cuts at: 0 712 1455
Size: 712 743
BsrFI r'CCGG_y
Cuts at: 0 29 1455
Size: 29 1426
BssHII G'CGCG_C
Cuts at: 0 25 1455
Size: 25 1430
BssSI C'ACGA_G
Cuts at: 0 319 937 1455
Size: 319 618 518
Fragments arranged by size:
618 518 319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 1455
Size: 48 1407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 1231 1455
Size: 621 610 224
Fragments arranged by size:
621 610 224
BstYI r'GATC_y
Cuts at: 0 152 1455
Size: 152 1303
BtgI C'CryG_G
Cuts at: 0 55 68 384 1115 1455
Size: 55 13 316 731 340
Fragments arranged by size:
731 340 316 55 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 861 1116 1455
Size: 861 255 339
Fragments arranged by size:
861 339 255
BtsI GCAGTG_nn'
Cuts at: 0 1063 1455
Size: 1063 392
EaeI y'GGCC_r
Cuts at: 0 204 349 566 1055 1118 1352 1455
Size: 204 145 217 489 63 234 103
Fragments arranged by size:
489 234 217 204 145 103 63
EagI C'GGCC_G
Cuts at: 0 204 1455
Size: 204 1251
EarI CTCTTCn'nnn_
Cuts at: 0 145 196 1399 1455
Size: 145 51 1203 56
Fragments arranged by size:
1203 145 56 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 282 1455
Size: 282 1173
EcoICRI GAG'CTC
Cuts at: 0 219 1455
Size: 219 1236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 705 1444 1455
Size: 597 108 739 11
Fragments arranged by size:
739 597 108 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1455
Size: 1222 233
EcoO109I rG'GnC_Cy
Cuts at: 0 120 177 502 1186 1455
Size: 120 57 325 684 269
Fragments arranged by size:
684 325 269 120 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 207 1455
Size: 207 1248
HaeII r_GCGC'y
Cuts at: 0 845 1361 1455
Size: 845 516 94
Fragments arranged by size:
845 516 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1455
Size: 1053 32 370
Fragments arranged by size:
1053 370 32
KasI G'GCGC_C
Cuts at: 0 841 1455
Size: 841 614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1455
Size: 416 1039
MslI CAynn'nnrTG
Cuts at: 0 247 925 1102 1455
Size: 247 678 177 353
Fragments arranged by size:
678 353 247 177
MspA1I CmG'CkG
Cuts at: 0 57 80 386 687 909 925 1117 1422
Size: 57 23 306 301 222 16 192 305
Cuts at: 1422 1455
Size: 33
Fragments arranged by size:
306 305 301 222 192 57 33 23
16
NaeI GCC'GGC
Cuts at: 0 31 1455
Size: 31 1424
NarI GG'CG_CC
Cuts at: 0 842 1455
Size: 842 613
NgoMIV G'CCGG_C
Cuts at: 0 29 1455
Size: 29 1426
NspI r_CATG'y
Cuts at: 0 669 1455
Size: 669 786
PpuMI rG'GwC_Cy
Cuts at: 0 120 177 1186 1455
Size: 120 57 1009 269
Fragments arranged by size:
1009 269 120 57
PshAI GACnn'nnGTC
Cuts at: 0 769 1455
Size: 769 686
PspOMI G'GGCC_C
Cuts at: 0 82 1455
Size: 82 1373
PstI C_TGCA'G
Cuts at: 0 53 1169 1455
Size: 53 1116 286
Fragments arranged by size:
1116 286 53
PvuII CAG'CTG
Cuts at: 0 687 1422 1455
Size: 687 735 33
Fragments arranged by size:
735 687 33
SacI G_AGCT'C
Cuts at: 0 221 1455
Size: 221 1234
SacII CC_GC'GG
Cuts at: 0 58 387 1118 1455
Size: 58 329 731 337
Fragments arranged by size:
731 337 329 58
SapI GCTCTTCn'nnn_
Cuts at: 0 196 1455
Size: 196 1259
SfcI C'TryA_G
Cuts at: 0 49 729 1165 1455
Size: 49 680 436 290
Fragments arranged by size:
680 436 290 49
SfoI GGC'GCC
Cuts at: 0 843 1455
Size: 843 612
SmaI CCC'GGG
Cuts at: 0 96 1442 1455
Size: 96 1346 13
Fragments arranged by size:
1346 96 13
SmlI C'TyrA_G
Cuts at: 0 181 1455
Size: 181 1274
SphI G_CATG'C
Cuts at: 0 669 1455
Size: 669 786
StyI C'CwwG_G
Cuts at: 0 1286 1455
Size: 1286 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 1455
Size: 888 567
TatI w'GTAC_w
Cuts at: 0 1094 1455
Size: 1094 361
Tth111I GACn'n_nGTC
Cuts at: 0 702 1455
Size: 702 753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 580 1293 1455
Size: 580 713 162
Fragments arranged by size:
713 580 162
XmaI C'CCGG_G
Cuts at: 0 94 1440 1455
Size: 94 1346 15
Fragments arranged by size:
1346 94 15
XmnI GAAnn'nnTTC
Cuts at: 0 406 1387 1455
Size: 406 981 68
Fragments arranged by size:
981 406 68
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI AscI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BpmI BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SapI SfcI SfoI SmaI SmlI SphI StyI
TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TstI XbaI XhoI ZraI