Restriction map for
pFN21AE5612
(Linear) (Six-base) MAPSORT of: KIEE5612.seq Check: 2278 from: 1 to: 1533
KIEE5612.orf
With 182 enzymes: SgfI *
December 5, 2014 13:03 ..
AccI GT'mk_AC
Cuts at: 0 410 967 1533
Size: 410 557 566
Fragments arranged by size:
566 557 410
ApoI r'AATT_y
Cuts at: 0 13 108 172 187 571 940 1176 1294
Size: 13 95 64 15 384 369 236 118
Cuts at: 1294 1436 1533
Size: 142 97
Fragments arranged by size:
384 369 236 142 118 97 95 64
15 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1277 1533
Size: 1277 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 1001 1533
Size: 967 34 532
Fragments arranged by size:
967 532 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 69 1533
Size: 69 1464
BclI T'GATC_A
Cuts at: 0 927 1533
Size: 927 606
BmrI ACTGGGnnnn_n'
Cuts at: 0 447 1533
Size: 447 1086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1533
Size: 1297 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1246 1341 1533
Size: 935 311 95 192
Fragments arranged by size:
935 311 192 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 444 1533
Size: 444 1089
BsaAI yAC'GTr
Cuts at: 0 1252 1533
Size: 1252 281
BsaWI w'CCGG_w
Cuts at: 0 663 894 1533
Size: 663 231 639
Fragments arranged by size:
663 639 231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 311 341 1533
Size: 311 30 1192
Fragments arranged by size:
1192 311 30
BsiHKAI G_wGCw'C
Cuts at: 0 120 1533
Size: 120 1413
BsmI GAATG_Cn'
Cuts at: 0 165 1533
Size: 165 1368
Bsp1286I G_dGCh'C
Cuts at: 0 120 1533
Size: 120 1413
BspEI T'CCGG_A
Cuts at: 0 663 1533
Size: 663 870
BsrGI T'GTAC_A
Cuts at: 0 528 1533
Size: 528 1005
BstZ17I GTA'TAC
Cuts at: 0 968 1533
Size: 968 565
BtgI C'CryG_G
Cuts at: 0 1415 1533
Size: 1415 118
BtsI GCAGTG_nn'
Cuts at: 0 767 1533
Size: 767 766
EaeI y'GGCC_r
Cuts at: 0 631 1533
Size: 631 902
EarI CTCTTCn'nnn_
Cuts at: 0 220 1533
Size: 220 1313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1533
Size: 1297 236
EcoRI G'AATT_C
Cuts at: 0 1176 1533
Size: 1176 357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 372 976 1533
Size: 42 330 604 557
Fragments arranged by size:
604 557 330 42
FspI TGC'GCA
Cuts at: 0 835 1533
Size: 835 698
HindIII A'AGCT_T
Cuts at: 0 36 1533
Size: 36 1497
MspA1I CmG'CkG
Cuts at: 0 749 1307 1533
Size: 749 558 226
Fragments arranged by size:
749 558 226
NcoI C'CATG_G
Cuts at: 0 1415 1533
Size: 1415 118
PvuII CAG'CTG
Cuts at: 0 749 1307 1533
Size: 749 558 226
Fragments arranged by size:
749 558 226
SfcI C'TryA_G
Cuts at: 0 812 1533
Size: 812 721
SmlI C'TyrA_G
Cuts at: 0 950 1225 1320 1533
Size: 950 275 95 213
Fragments arranged by size:
950 275 213 95
SspI AAT'ATT
Cuts at: 0 269 1533
Size: 269 1264
StyI C'CwwG_G
Cuts at: 0 1415 1533
Size: 1415 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 982 1533
Size: 982 551
TatI w'GTAC_w
Cuts at: 0 528 1533
Size: 528 1005
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 1167 1199 1533
Size: 311 32 824 32 334
Fragments arranged by size:
824 334 311 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 641 1533
Size: 641 892
XmnI GAAnn'nnTTC
Cuts at: 0 512 592 1533
Size: 512 80 941
Fragments arranged by size:
941 512 80
Enzymes that do cut:
AccI ApoI BbsI BcgI BciVI BclI BmrI BpmI
BpuEI BsaI BsaAI BsaWI BsaXI BsiHKAI BsmI Bsp1286I
BspEI BsrGI BstZ17I BtgI BtsI EaeI EarI Eco57MI
EcoRI FalI FspI HindIII MspA1I NcoI PvuII SfcI
SmlI SspI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI