Restriction map for
pFN21AE5604
(Linear) (Six-base) MAPSORT of: KIEE5604.seq Check: 8751 from: 1 to: 1431
KIEE5604.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
Acc65I G'GTAC_C
Cuts at: 0 46 527 947 1431
Size: 46 481 420 484
Fragments arranged by size:
484 481 420 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1408 1431
Size: 973 435 23
Fragments arranged by size:
973 435 23
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 794 826 1431
Size: 794 32 605
Fragments arranged by size:
794 605 32
AlwNI CAG_nnn'CTG
Cuts at: 0 543 1431
Size: 543 888
ApoI r'AATT_y
Cuts at: 0 1198 1401 1431
Size: 1198 203 30
Fragments arranged by size:
1198 203 30
AscI GG'CGCG_CC
Cuts at: 0 349 1431
Size: 349 1082
AvaI C'yCGr_G
Cuts at: 0 96 131 363 631 1268 1431
Size: 96 35 232 268 637 163
Fragments arranged by size:
637 268 232 163 96 35
AvrII C'CTAG_G
Cuts at: 0 435 1431
Size: 435 996
BanI G'GyrC_C
Cuts at: 0 6 46 340 527 947 1431
Size: 6 40 294 187 420 484
Fragments arranged by size:
484 420 294 187 40 6
BanII G_rGCy'C
Cuts at: 0 182 191 429 631 1431
Size: 182 9 238 202 800
Fragments arranged by size:
800 238 202 182 9
BbeI G_GCGC'C
Cuts at: 0 10 344 1431
Size: 10 334 1087
Fragments arranged by size:
1087 334 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 146 1431
Size: 112 34 1285
Fragments arranged by size:
1285 112 34
BclI T'GATC_A
Cuts at: 0 735 987 1155 1431
Size: 735 252 168 276
Fragments arranged by size:
735 276 252 168
BglI GCCn_nnn'nGGC
Cuts at: 0 78 454 635 1023 1431
Size: 78 376 181 388 408
Fragments arranged by size:
408 388 376 181 78
BlpI GC'TnA_GC
Cuts at: 0 595 1431
Size: 595 836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 655 806 854 1162 1431
Size: 415 240 151 48 308 269
Fragments arranged by size:
415 308 269 240 151 48
Bpu10I CC'TnA_GC
Cuts at: 0 104 1431
Size: 104 1327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 607 1348 1431
Size: 463 144 741 83
Fragments arranged by size:
741 463 144 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 353 1431
Size: 353 1078
BsaHI Gr'CG_yC
Cuts at: 0 7 341 1431
Size: 7 334 1090
Fragments arranged by size:
1090 334 7
BsaWI w'CCGG_w
Cuts at: 0 757 927 1431
Size: 757 170 504
Fragments arranged by size:
757 504 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 779 809 1135 1165 1431
Size: 779 30 326 30 266
Fragments arranged by size:
779 326 266 30 30
BseYI C'CCAG_C
Cuts at: 0 285 723 843 1431
Size: 285 438 120 588
Fragments arranged by size:
588 438 285 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1431
Size: 631 800
BsiEI CG_ry'CG
Cuts at: 0 198 372 1431
Size: 198 174 1059
Fragments arranged by size:
1059 198 174
BsiHKAI G_wGCw'C
Cuts at: 0 182 191 429 671 1431
Size: 182 9 238 242 760
Fragments arranged by size:
760 242 238 182 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 1431
Size: 154 1277
Bsp1286I G_dGCh'C
Cuts at: 0 182 191 429 631 671 1431
Size: 182 9 238 202 40 760
Fragments arranged by size:
760 238 202 182 40 9
BspEI T'CCGG_A
Cuts at: 0 757 1431
Size: 757 674
BsrBI CCG'CTC
Cuts at: 0 310 368 1431
Size: 310 58 1063
Fragments arranged by size:
1063 310 58
BsrFI r'CCGG_y
Cuts at: 0 35 599 1431
Size: 35 564 832
Fragments arranged by size:
832 564 35
BsrGI T'GTAC_A
Cuts at: 0 1105 1431
Size: 1105 326
BssHII G'CGCG_C
Cuts at: 0 349 1431
Size: 349 1082
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 1431
Size: 609 822
BstXI CCAn_nnnn'nTGG
Cuts at: 0 706 1431
Size: 706 725
BstYI r'GATC_y
Cuts at: 0 730 1431
Size: 730 701
BtgI C'CryG_G
Cuts at: 0 198 245 344 1431
Size: 198 47 99 1087
Fragments arranged by size:
1087 198 99 47
BtsI GCAGTG_nn'
Cuts at: 0 569 1431
Size: 569 862
DraI TTT'AAA
Cuts at: 0 1179 1431
Size: 1179 252
DraIII CAC_nnn'GTG
Cuts at: 0 321 546 1431
Size: 321 225 885
Fragments arranged by size:
885 321 225
EaeI y'GGCC_r
Cuts at: 0 369 647 1431
Size: 369 278 784
Fragments arranged by size:
784 369 278
EagI C'GGCC_G
Cuts at: 0 369 1431
Size: 369 1062
EarI CTCTTCn'nnn_
Cuts at: 0 196 700 1431
Size: 196 504 731
Fragments arranged by size:
731 504 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 743 1310 1431
Size: 743 567 121
Fragments arranged by size:
743 567 121
EcoICRI GAG'CTC
Cuts at: 0 180 189 427 1431
Size: 180 9 238 1004
Fragments arranged by size:
1004 238 180 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 655 806 854 973 1162 1408 1431
Size: 415 240 151 48 119 189 246 23
Fragments arranged by size:
415 246 240 189 151 119 48 23
EcoO109I rG'GnC_Cy
Cuts at: 0 447 1030 1431
Size: 447 583 401
Fragments arranged by size:
583 447 401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 1431
Size: 168 1263
HaeII r_GCGC'y
Cuts at: 0 10 104 344 394 489 1431
Size: 10 94 240 50 95 942
Fragments arranged by size:
942 240 95 94 50 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 701 733 874 906 1135 1167
Size: 472 32 197 32 141 32 229 32
Cuts at: 1167 1431
Size: 264
Fragments arranged by size:
472 264 229 197 141 32 32 32
32
HincII GTy'rAC
Cuts at: 0 782 1431
Size: 782 649
HindIII A'AGCT_T
Cuts at: 0 1063 1431
Size: 1063 368
KasI G'GCGC_C
Cuts at: 0 6 340 1431
Size: 6 334 1091
Fragments arranged by size:
1091 334 6
KpnI G_GTAC'C
Cuts at: 0 50 531 951 1431
Size: 50 481 420 480
Fragments arranged by size:
481 480 420 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1329 1431
Size: 1329 102
MspA1I CmG'CkG
Cuts at: 0 615 723 1002 1431
Size: 615 108 279 429
Fragments arranged by size:
615 429 279 108
NaeI GCC'GGC
Cuts at: 0 601 1431
Size: 601 830
NarI GG'CG_CC
Cuts at: 0 7 341 1431
Size: 7 334 1090
Fragments arranged by size:
1090 334 7
NgoMIV G'CCGG_C
Cuts at: 0 599 1431
Size: 599 832
NspI r_CATG'y
Cuts at: 0 1009 1431
Size: 1009 422
PfoI T'CCnGG_A
Cuts at: 0 155 1212 1431
Size: 155 1057 219
Fragments arranged by size:
1057 219 155
PpuMI rG'GwC_Cy
Cuts at: 0 1030 1431
Size: 1030 401
PsiI TTA'TAA
Cuts at: 0 1414 1431
Size: 1414 17
PspXI vC'TCGA_Gb
Cuts at: 0 131 1431
Size: 131 1300
PvuII CAG'CTG
Cuts at: 0 615 723 1002 1431
Size: 615 108 279 429
Fragments arranged by size:
615 429 279 108
SacI G_AGCT'C
Cuts at: 0 182 191 429 1431
Size: 182 9 238 1002
Fragments arranged by size:
1002 238 182 9
SapI GCTCTTCn'nnn_
Cuts at: 0 196 1431
Size: 196 1235
SexAI A'CCwGG_T
Cuts at: 0 542 662 1431
Size: 542 120 769
Fragments arranged by size:
769 542 120
SfoI GGC'GCC
Cuts at: 0 8 342 1431
Size: 8 334 1089
Fragments arranged by size:
1089 334 8
SmlI C'TyrA_G
Cuts at: 0 131 442 622 1363 1431
Size: 131 311 180 741 68
Fragments arranged by size:
741 311 180 131 68
StuI AGG'CCT
Cuts at: 0 676 1431
Size: 676 755
StyI C'CwwG_G
Cuts at: 0 74 377 435 1431
Size: 74 303 58 996
Fragments arranged by size:
996 303 74 58
TatI w'GTAC_w
Cuts at: 0 826 1105 1431
Size: 826 279 326
Fragments arranged by size:
826 326 279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 1431
Size: 351 1080
XhoI C'TCGA_G
Cuts at: 0 131 1431
Size: 131 1300
XmnI GAAnn'nnTTC
Cuts at: 0 958 1202 1431
Size: 958 244 229
Fragments arranged by size:
958 244 229
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApoI AscI AvaI AvrII
BanI BanII BbeI BcgI BclI BglI BlpI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrFI BsrGI
BssHII BstAPI BstXI BstYI BtgI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MspA1I
NaeI NarI NgoMIV NspI PfoI PpuMI PsiI PspXI
PvuII SacI SapI SexAI SfoI SmlI StuI StyI
TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AseI AsiSI BaeI
BamHI BbsI BbvCI BciVI BfrBI BglII Bme1580I BmgBI
BmrI BmtI BplI BsaAI BsaBI BseRI BsiWI BsmI
BspHI BspMI BsrDI BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI MfeI MluI MscI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspOMI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XmaI ZraI