Restriction map for
pFN21AE5602
(Linear) (Six-base) MAPSORT of: KIEE5602.seq Check: 6272 from: 1 to: 1737
KIEE5602.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1438 1605 1737
Size: 1438 167 132
Fragments arranged by size:
1438 167 132
AccI GT'mk_AC
Cuts at: 0 226 427 1737
Size: 226 201 1310
Fragments arranged by size:
1310 226 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 778 1130 1737
Size: 595 183 352 607
Fragments arranged by size:
607 595 352 183
AflIII A'CryG_T
Cuts at: 0 1441 1737
Size: 1441 296
AleI CACnn'nnGTG
Cuts at: 0 1400 1737
Size: 1400 337
AseI AT'TA_AT
Cuts at: 0 1184 1737
Size: 1184 553
AvrII C'CTAG_G
Cuts at: 0 330 1737
Size: 330 1407
BanII G_rGCy'C
Cuts at: 0 290 330 439 1737
Size: 290 40 109 1298
Fragments arranged by size:
1298 290 109 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 766 781 1737
Size: 766 15 956
Fragments arranged by size:
956 766 15
BbvCI CC'TCA_GC
Cuts at: 0 911 1737
Size: 911 826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1062 1096 1737
Size: 1062 34 641
Fragments arranged by size:
1062 641 34
BfrBI ATG'CAT
Cuts at: 0 139 1737
Size: 139 1598
BlpI GC'TnA_GC
Cuts at: 0 274 1037 1610 1737
Size: 274 763 573 127
Fragments arranged by size:
763 573 274 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 164 371 1153 1737
Size: 164 207 782 584
Fragments arranged by size:
782 584 207 164
BmtI G_CTAG'C
Cuts at: 0 731 1737
Size: 731 1006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1737
Size: 665 1072
Bpu10I CC'TnA_GC
Cuts at: 0 911 953 1737
Size: 911 42 784
Fragments arranged by size:
911 784 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1737
Size: 132 1605
BsaI GGTCTCn'nnnn_
Cuts at: 0 21 514 911 1737
Size: 21 493 397 826
Fragments arranged by size:
826 493 397 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 678 708 1737
Size: 169 30 479 30 1029
Fragments arranged by size:
1029 479 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 686 899 1737
Size: 686 213 838
Fragments arranged by size:
838 686 213
BseYI C'CCAG_C
Cuts at: 0 1010 1049 1737
Size: 1010 39 688
Fragments arranged by size:
1010 688 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1317 1466 1737
Size: 1317 149 271
Fragments arranged by size:
1317 271 149
BsiHKAI G_wGCw'C
Cuts at: 0 439 1737
Size: 439 1298
Bsp1286I G_dGCh'C
Cuts at: 0 290 330 439 1737
Size: 290 40 109 1298
Fragments arranged by size:
1298 290 109 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1438 1605 1737
Size: 1438 167 132
Fragments arranged by size:
1438 167 132
BsrDI GCAATG_nn'
Cuts at: 0 314 609 689 773 982 1737
Size: 314 295 80 84 209 755
Fragments arranged by size:
755 314 295 209 84 80
BsrGI T'GTAC_A
Cuts at: 0 66 1737
Size: 66 1671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 145 1737
Size: 145 1592
BstXI CCAn_nnnn'nTGG
Cuts at: 0 713 1049 1737
Size: 713 336 688
Fragments arranged by size:
713 688 336
BstYI r'GATC_y
Cuts at: 0 61 1135 1737
Size: 61 1074 602
Fragments arranged by size:
1074 602 61
Bsu36I CC'TnA_GG
Cuts at: 0 477 1737
Size: 477 1260
BtgI C'CryG_G
Cuts at: 0 1068 1737
Size: 1068 669
BtsI GCAGTG_nn'
Cuts at: 0 1080 1395 1737
Size: 1080 315 342
Fragments arranged by size:
1080 342 315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1371 1406 1737
Size: 1371 35 331
Fragments arranged by size:
1371 331 35
DraIII CAC_nnn'GTG
Cuts at: 0 1161 1737
Size: 1161 576
DrdI GACnn_nn'nnGTC
Cuts at: 0 35 1737
Size: 35 1702
EaeI y'GGCC_r
Cuts at: 0 5 1227 1737
Size: 5 1222 510
Fragments arranged by size:
1222 510 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 782 1737
Size: 782 955
EcoICRI GAG'CTC
Cuts at: 0 437 1737
Size: 437 1300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 665 778 1130 1737
Size: 595 70 113 352 607
Fragments arranged by size:
607 595 352 113 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 529 1737
Size: 529 1208
EcoO109I rG'GnC_Cy
Cuts at: 0 844 1357 1737
Size: 844 513 380
Fragments arranged by size:
844 513 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 1654 1686 1737
Size: 487 32 1135 32 51
Fragments arranged by size:
1135 487 51 32 32
HincII GTy'rAC
Cuts at: 0 1383 1737
Size: 1383 354
HindIII A'AGCT_T
Cuts at: 0 1171 1737
Size: 1171 566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 1737
Size: 1145 592
MslI CAynn'nnrTG
Cuts at: 0 244 1400 1737
Size: 244 1156 337
Fragments arranged by size:
1156 337 244
MspA1I CmG'CkG
Cuts at: 0 273 1049 1737
Size: 273 776 688
Fragments arranged by size:
776 688 273
NheI G'CTAG_C
Cuts at: 0 727 1737
Size: 727 1010
NsiI A_TGCA'T
Cuts at: 0 141 1737
Size: 141 1596
NspI r_CATG'y
Cuts at: 0 1445 1737
Size: 1445 292
PasI CC'CwG_GG
Cuts at: 0 900 1737
Size: 900 837
PciI A'CATG_T
Cuts at: 0 1441 1737
Size: 1441 296
PflMI CCAn_nnn'nTGG
Cuts at: 0 349 564 1737
Size: 349 215 1173
Fragments arranged by size:
1173 349 215
PpuMI rG'GwC_Cy
Cuts at: 0 844 1737
Size: 844 893
PvuII CAG'CTG
Cuts at: 0 273 1737
Size: 273 1464
SacI G_AGCT'C
Cuts at: 0 439 1737
Size: 439 1298
SanDI GG'GwC_CC
Cuts at: 0 844 1737
Size: 844 893
SfcI C'TryA_G
Cuts at: 0 177 551 917 1737
Size: 177 374 366 820
Fragments arranged by size:
820 374 366 177
SmlI C'TyrA_G
Cuts at: 0 111 1737
Size: 111 1626
SpeI A'CTAG_T
Cuts at: 0 649 1737
Size: 649 1088
SspI AAT'ATT
Cuts at: 0 1290 1737
Size: 1290 447
StyI C'CwwG_G
Cuts at: 0 330 558 1737
Size: 330 228 1179
Fragments arranged by size:
1179 330 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 1072 1166 1172 1737
Size: 199 873 94 6 565
Fragments arranged by size:
873 565 199 94 6
TatI w'GTAC_w
Cuts at: 0 51 66 1737
Size: 51 15 1671
Fragments arranged by size:
1671 51 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 678 710 1636 1668 1737
Size: 678 32 926 32 69
Fragments arranged by size:
926 678 69 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1737
Size: 15 1722
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AseI AvrII BanII
BbsI BbvCI BcgI BfrBI BlpI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII DrdI EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII MmeI MslI
MspA1I NheI NsiI NspI PasI PciI PflMI PpuMI
PvuII SacI SanDI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AsiSI AvaI
BaeI BamHI BanI BbeI BciVI BclI BglI BglII
Bme1580I BmgBI BplI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI DraI EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI