Restriction map for
pFN21AE5588
(Linear) (Six-base) MAPSORT of: KIEE5588.seq Check: 472 from: 1 to: 1131
KIEE5588.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 499 1131
Size: 499 632
AgeI A'CCGG_T
Cuts at: 0 1007 1131
Size: 1007 124
AlwNI CAG_nnn'CTG
Cuts at: 0 318 804 1131
Size: 318 486 327
Fragments arranged by size:
486 327 318
ApoI r'AATT_y
Cuts at: 0 127 1131
Size: 127 1004
AvaI C'yCGr_G
Cuts at: 0 51 679 754 778 820 1131
Size: 51 628 75 24 42 311
Fragments arranged by size:
628 311 75 51 42 24
BanI G'GyrC_C
Cuts at: 0 119 774 1131
Size: 119 655 357
Fragments arranged by size:
655 357 119
BanII G_rGCy'C
Cuts at: 0 335 575 680 820 827 1131
Size: 335 240 105 140 7 304
Fragments arranged by size:
335 304 240 140 105 7
BbeI G_GCGC'C
Cuts at: 0 778 1131
Size: 778 353
BbvCI CC'TCA_GC
Cuts at: 0 141 1131
Size: 141 990
BclI T'GATC_A
Cuts at: 0 236 1131
Size: 236 895
Bme1580I G_kGCm'C
Cuts at: 0 122 401 797 913 1131
Size: 122 279 396 116 218
Fragments arranged by size:
396 279 218 122 116
BmgBI CAC'GTC
Cuts at: 0 255 1131
Size: 255 876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 534 1131
Size: 534 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 976 1131
Size: 825 151 155
Fragments arranged by size:
825 155 151
Bpu10I CC'TnA_GC
Cuts at: 0 141 1131
Size: 141 990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1131
Size: 931 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 637 843 1131
Size: 637 206 288
Fragments arranged by size:
637 288 206
BsaAI yAC'GTr
Cuts at: 0 549 666 1131
Size: 549 117 465
Fragments arranged by size:
549 465 117
BsaHI Gr'CG_yC
Cuts at: 0 775 1131
Size: 775 356
BsaWI w'CCGG_w
Cuts at: 0 1007 1131
Size: 1007 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 1131
Size: 172 30 929
Fragments arranged by size:
929 172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 565 721 1131
Size: 193 372 156 410
Fragments arranged by size:
410 372 193 156
BseYI C'CCAG_C
Cuts at: 0 304 797 804 1131
Size: 304 493 7 327
Fragments arranged by size:
493 327 304 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 477 751 1131
Size: 196 281 274 380
Fragments arranged by size:
380 281 274 196
BsiEI CG_ry'CG
Cuts at: 0 1008 1131
Size: 1008 123
BsiHKAI G_wGCw'C
Cuts at: 0 575 1131
Size: 575 556
Bsp1286I G_dGCh'C
Cuts at: 0 122 335 401 575 680 797 820 827
Size: 122 213 66 174 105 117 23 7
Cuts at: 827 913 1131
Size: 86 218
Fragments arranged by size:
218 213 174 122 117 105 86 66
23 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 499 1131
Size: 499 632
BsrFI r'CCGG_y
Cuts at: 0 938 1007 1131
Size: 938 69 124
Fragments arranged by size:
938 124 69
BsrGI T'GTAC_A
Cuts at: 0 188 1131
Size: 188 943
BssSI C'ACGA_G
Cuts at: 0 634 1131
Size: 634 497
BstEII G'GTnAC_C
Cuts at: 0 486 1074 1131
Size: 486 588 57
Fragments arranged by size:
588 486 57
BstYI r'GATC_y
Cuts at: 0 878 1131
Size: 878 253
Bsu36I CC'TnA_GG
Cuts at: 0 715 1131
Size: 715 416
BtgI C'CryG_G
Cuts at: 0 924 1109 1131
Size: 924 185 22
Fragments arranged by size:
924 185 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1008 1043 1131
Size: 1008 35 88
Fragments arranged by size:
1008 88 35
DraIII CAC_nnn'GTG
Cuts at: 0 245 857 1131
Size: 245 612 274
Fragments arranged by size:
612 274 245
EaeI y'GGCC_r
Cuts at: 0 593 600 1131
Size: 593 7 531
Fragments arranged by size:
593 531 7
EcoICRI GAG'CTC
Cuts at: 0 573 1131
Size: 573 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 976 1131
Size: 825 151 155
Fragments arranged by size:
825 155 151
EcoO109I rG'GnC_Cy
Cuts at: 0 183 201 711 759 783 1103 1131
Size: 183 18 510 48 24 320 28
Fragments arranged by size:
510 320 183 48 28 24 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 294 900 1131
Size: 183 111 606 231
Fragments arranged by size:
606 231 183 111
HaeII r_GCGC'y
Cuts at: 0 778 1131
Size: 778 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 981 1013 1022 1054 1131
Size: 34 32 915 32 9 32 77
Fragments arranged by size:
915 77 34 32 32 32 9
KasI G'GCGC_C
Cuts at: 0 774 1131
Size: 774 357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 1131
Size: 395 736
MscI TGG'CCA
Cuts at: 0 595 1131
Size: 595 536
MspA1I CmG'CkG
Cuts at: 0 210 476 585 599 621 765 804 963
Size: 210 266 109 14 22 144 39 159
Cuts at: 963 1131
Size: 168
Fragments arranged by size:
266 210 168 159 144 109 39 22
14
NarI GG'CG_CC
Cuts at: 0 775 1131
Size: 775 356
PasI CC'CwG_GG
Cuts at: 0 347 1131
Size: 347 784
PflMI CCAn_nnn'nTGG
Cuts at: 0 804 930 1131
Size: 804 126 201
Fragments arranged by size:
804 201 126
PfoI T'CCnGG_A
Cuts at: 0 694 1131
Size: 694 437
PmlI CAC'GTG
Cuts at: 0 549 1131
Size: 549 582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1131
Size: 772 32 327
Fragments arranged by size:
772 327 32
PpuMI rG'GwC_Cy
Cuts at: 0 711 759 1103 1131
Size: 711 48 344 28
Fragments arranged by size:
711 344 48 28
PshAI GACnn'nnGTC
Cuts at: 0 629 1131
Size: 629 502
PstI C_TGCA'G
Cuts at: 0 209 1131
Size: 209 922
PvuII CAG'CTG
Cuts at: 0 210 585 621 804 963 1131
Size: 210 375 36 183 159 168
Fragments arranged by size:
375 210 183 168 159 36
SacI G_AGCT'C
Cuts at: 0 575 1131
Size: 575 556
SanDI GG'GwC_CC
Cuts at: 0 759 1131
Size: 759 372
ScaI AGT'ACT
Cuts at: 0 166 1131
Size: 166 965
SfcI C'TryA_G
Cuts at: 0 205 1131
Size: 205 926
SfoI GGC'GCC
Cuts at: 0 776 1131
Size: 776 355
SmaI CCC'GGG
Cuts at: 0 822 1131
Size: 822 309
SmlI C'TyrA_G
Cuts at: 0 946 1131
Size: 946 185
StyI C'CwwG_G
Cuts at: 0 671 971 1131
Size: 671 300 160
Fragments arranged by size:
671 300 160
TatI w'GTAC_w
Cuts at: 0 164 188 722 1131
Size: 164 24 534 409
Fragments arranged by size:
534 409 164 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 1131
Size: 546 585
XmaI C'CCGG_G
Cuts at: 0 820 1131
Size: 820 311
Enzymes that do cut:
AarI AgeI AlwNI ApoI AvaI BanI BanII BbeI
BbvCI BclI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssSI BstEII
BstYI Bsu36I BtgI CspCI DraIII EaeI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MspA1I
NarI PasI PflMI PfoI PmlI PpiI PpuMI PshAI
PstI PvuII SacI SanDI ScaI SfcI SfoI SmaI
SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI
BglI BglII BlpI BmrI BmtI BsaBI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssHII BstAPI BstBI
BstXI BstZ17I BtgZI BtsI ClaI DraI DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI