Restriction map for
pFN21AE5586
(Linear) (Six-base) MAPSORT of: KIEE5586.seq Check: 7525 from: 1 to: 1113
KIEE5586.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AccI GT'mk_AC
Cuts at: 0 784 1113
Size: 784 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 952 1113
Size: 420 532 161
Fragments arranged by size:
532 420 161
AfeI AGC'GCT
Cuts at: 0 290 1113
Size: 290 823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 979 1113
Size: 979 134
AlwNI CAG_nnn'CTG
Cuts at: 0 99 184 741 840 1113
Size: 99 85 557 99 273
Fragments arranged by size:
557 273 99 99 85
AvaI C'yCGr_G
Cuts at: 0 275 463 589 1113
Size: 275 188 126 524
Fragments arranged by size:
524 275 188 126
BamHI G'GATC_C
Cuts at: 0 207 585 932 1113
Size: 207 378 347 181
Fragments arranged by size:
378 347 207 181
BanI G'GyrC_C
Cuts at: 0 340 1113
Size: 340 773
BanII G_rGCy'C
Cuts at: 0 577 1068 1113
Size: 577 491 45
Fragments arranged by size:
577 491 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 966 1113
Size: 966 147
BbvCI CC'TCA_GC
Cuts at: 0 382 1113
Size: 382 731
BglI GCCn_nnn'nGGC
Cuts at: 0 51 86 652 1113
Size: 51 35 566 461
Fragments arranged by size:
566 461 51 35
BlpI GC'TnA_GC
Cuts at: 0 571 1113
Size: 571 542
Bme1580I G_kGCm'C
Cuts at: 0 47 227 905 1113
Size: 47 180 678 208
Fragments arranged by size:
678 208 180 47
BmgBI CAC'GTC
Cuts at: 0 252 1113
Size: 252 861
BmtI G_CTAG'C
Cuts at: 0 136 1113
Size: 136 977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 418 848 1033 1113
Size: 206 212 430 185 80
Fragments arranged by size:
430 212 206 185 80
Bpu10I CC'TnA_GC
Cuts at: 0 382 1113
Size: 382 731
BsaAI yAC'GTr
Cuts at: 0 42 774 1113
Size: 42 732 339
Fragments arranged by size:
732 339 42
BsaHI Gr'CG_yC
Cuts at: 0 260 1113
Size: 260 853
BseYI C'CCAG_C
Cuts at: 0 226 291 594 656 1113
Size: 226 65 303 62 457
Fragments arranged by size:
457 303 226 65 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 1113
Size: 486 627
BsiHKAI G_wGCw'C
Cuts at: 0 1068 1113
Size: 1068 45
Bsp1286I G_dGCh'C
Cuts at: 0 47 227 577 905 1068 1113
Size: 47 180 350 328 163 45
Fragments arranged by size:
350 328 180 163 47 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 1113
Size: 80 1033
BsrFI r'CCGG_y
Cuts at: 0 262 373 1113
Size: 262 111 740
Fragments arranged by size:
740 262 111
BssHII G'CGCG_C
Cuts at: 0 402 1113
Size: 402 711
BssSI C'ACGA_G
Cuts at: 0 904 1113
Size: 904 209
BstYI r'GATC_y
Cuts at: 0 207 585 887 932 1113
Size: 207 378 302 45 181
Fragments arranged by size:
378 302 207 181 45
BstZ17I GTA'TAC
Cuts at: 0 785 1113
Size: 785 328
BtgI C'CryG_G
Cuts at: 0 356 414 1113
Size: 356 58 699
Fragments arranged by size:
699 356 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 15 1113
Size: 15 1098
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1026 1061 1113
Size: 1026 35 52
Fragments arranged by size:
1026 52 35
DraIII CAC_nnn'GTG
Cuts at: 0 222 1113
Size: 222 891
EaeI y'GGCC_r
Cuts at: 0 2 1113
Size: 2 1111
EarI CTCTTCn'nnn_
Cuts at: 0 88 1113
Size: 88 1025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 1113
Size: 271 842
EcoICRI GAG'CTC
Cuts at: 0 1066 1113
Size: 1066 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 418 420 848 952 1033 1113
Size: 206 212 2 428 104 81 80
Fragments arranged by size:
428 212 206 104 81 80 2
FspI TGC'GCA
Cuts at: 0 142 1113
Size: 142 971
HaeII r_GCGC'y
Cuts at: 0 81 233 269 292 1113
Size: 81 152 36 23 821
Fragments arranged by size:
821 152 81 36 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 917 949 1113
Size: 917 32 164
Fragments arranged by size:
917 164 32
MspA1I CmG'CkG
Cuts at: 0 146 195 416 570 1113
Size: 146 49 221 154 543
Fragments arranged by size:
543 221 154 146 49
NaeI GCC'GGC
Cuts at: 0 264 375 1113
Size: 264 111 738
Fragments arranged by size:
738 264 111
NgoMIV G'CCGG_C
Cuts at: 0 262 373 1113
Size: 262 111 740
Fragments arranged by size:
740 262 111
NheI G'CTAG_C
Cuts at: 0 132 1113
Size: 132 981
PasI CC'CwG_GG
Cuts at: 0 850 1113
Size: 850 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 840 1113
Size: 99 741 273
Fragments arranged by size:
741 273 99
PfoI T'CCnGG_A
Cuts at: 0 1010 1113
Size: 1010 103
PmlI CAC'GTG
Cuts at: 0 42 774 1113
Size: 42 732 339
Fragments arranged by size:
732 339 42
PstI C_TGCA'G
Cuts at: 0 77 301 653 770 900 1113
Size: 77 224 352 117 130 213
Fragments arranged by size:
352 224 213 130 117 77
SacI G_AGCT'C
Cuts at: 0 1068 1113
Size: 1068 45
SacII CC_GC'GG
Cuts at: 0 417 1113
Size: 417 696
SbfI CC_TGCA'GG
Cuts at: 0 653 1113
Size: 653 460
SexAI A'CCwGG_T
Cuts at: 0 725 1113
Size: 725 388
SfcI C'TryA_G
Cuts at: 0 73 297 649 766 896 1113
Size: 73 224 352 117 130 217
Fragments arranged by size:
352 224 217 130 117 73
SgrAI Cr'CCGG_yG
Cuts at: 0 262 1113
Size: 262 851
SmaI CCC'GGG
Cuts at: 0 277 465 1113
Size: 277 188 648
Fragments arranged by size:
648 277 188
StuI AGG'CCT
Cuts at: 0 764 1113
Size: 764 349
StyI C'CwwG_G
Cuts at: 0 494 1113
Size: 494 619
Tth111I GACn'n_nGTC
Cuts at: 0 154 1113
Size: 154 959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 684 813 1113
Size: 354 330 129 300
Fragments arranged by size:
354 330 300 129
XmaI C'CCGG_G
Cuts at: 0 275 463 1113
Size: 275 188 650
Fragments arranged by size:
650 275 188
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI AvaI BamHI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmtI
BpmI Bpu10I BsaAI BsaHI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrFI BssHII BssSI BstYI BstZ17I BtgI BtgZI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI FspI
HaeII Hin4I MspA1I NaeI NgoMIV NheI PasI PflMI
PfoI PmlI PstI SacI SacII SbfI SexAI SfcI
SgrAI SmaI StuI StyI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII
BmrI BplI BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BstAPI BstBI BstEII BstXI Bsu36I BtsI ClaI
DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NarI NcoI NdeI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI ScaI SfiI SfoI SgfI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI XbaI XhoI XmnI ZraI