(Linear) (Six-base) MAPSORT of: KIEE5580.seq  Check: 6429  from: 1  to: 1071



KIEE5580.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1071 
   Size:        713     358
 
AflII C'TTAA_G

Cuts at:      0     490    1071 
   Size:        490     581
 
AflIII A'CryG_T

Cuts at:      0     147     718    1071 
   Size:        147     571     353

  Fragments arranged by size: 

                 571     353     147
 
ApoI r'AATT_y

Cuts at:      0    1063    1071 
   Size:       1063       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     328     916    1071 
   Size:        328     588     155

  Fragments arranged by size: 

                 588     328     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1071 
   Size:        538     533
 
BseYI C'CCAG_C

Cuts at:      0     296     793    1071 
   Size:        296     497     278

  Fragments arranged by size: 

                 497     296     278
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     785    1071 
   Size:        785     286
 
BsrDI GCAATG_nn'

Cuts at:      0     839    1071 
   Size:        839     232
 
BtgI C'CryG_G

Cuts at:      0      61    1071 
   Size:         61    1010
 
BtsI GCAGTG_nn'

Cuts at:      0     677    1071 
   Size:        677     394
 
DraI TTT'AAA

Cuts at:      0     903    1071 
   Size:        903     168
 
DraIII CAC_nnn'GTG

Cuts at:      0     505    1071 
   Size:        505     566
 
EaeI y'GGCC_r

Cuts at:      0     344    1071 
   Size:        344     727
 
EarI CTCTTCn'nnn_

Cuts at:      0     832    1071 
   Size:        832     239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1071 
   Size:        713     358
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     903    1071 
   Size:        903     168
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     915    1071 
   Size:        915     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     807     839    1071 
   Size:        807      32     232

  Fragments arranged by size: 

                 807     232      32
 
HincII GTy'rAC

Cuts at:      0     570     700    1071 
   Size:        570     130     371

  Fragments arranged by size: 

                 570     371     130
 
HindIII A'AGCT_T

Cuts at:      0     960    1071 
   Size:        960     111
 
MslI CAynn'nnrTG

Cuts at:      0     172     607    1071 
   Size:        172     435     464

  Fragments arranged by size: 

                 464     435     172
 
MspA1I CmG'CkG

Cuts at:      0     436    1071 
   Size:        436     635
 
NcoI C'CATG_G

Cuts at:      0      61    1071 
   Size:         61    1010
 
NdeI CA'TA_TG

Cuts at:      0     253    1071 
   Size:        253     818
 
NspI r_CATG'y

Cuts at:      0     151     722    1071 
   Size:        151     571     349

  Fragments arranged by size: 

                 571     349     151
 
PciI A'CATG_T

Cuts at:      0     147     718    1071 
   Size:        147     571     353

  Fragments arranged by size: 

                 571     353     147
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     505    1071 
   Size:        505     566
 
PfoI T'CCnGG_A

Cuts at:      0     203     442    1071 
   Size:        203     239     629

  Fragments arranged by size: 

                 629     239     203
 
PvuII CAG'CTG

Cuts at:      0     436    1071 
   Size:        436     635
 
SfcI C'TryA_G

Cuts at:      0    1031    1071 
   Size:       1031      40
 
SmlI C'TyrA_G

Cuts at:      0     490     553    1071 
   Size:        490      63     518

  Fragments arranged by size: 

                 518     490      63
 
StyI C'CwwG_G

Cuts at:      0      61     317    1071 
   Size:         61     256     754

  Fragments arranged by size: 

                 754     256      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     807     861    1071 
   Size:        807      54     210

  Fragments arranged by size: 

                 807     210      54
 
TatI w'GTAC_w

Cuts at:      0     176     226    1071 
   Size:        176      50     845

  Fragments arranged by size: 

                 845     176      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     335    1071 
   Size:        335     736
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     ApoI     BbsI    BpuEI    BseYI    BspMI
    BsrDI     BtgI     BtsI     DraI   DraIII     EaeI     EarI  Eco57MI
    EcoNI     FalI    Hin4I   HincII  HindIII     MslI   MspA1I     NcoI
     NdeI     NspI     PciI    PflMI     PfoI    PvuII     SfcI     SmlI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI     EagI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI