Restriction map for
pFN21AE5578
(Linear) (Six-base) MAPSORT of: KIEE5578.seq Check: 5336 from: 1 to: 1374
KIEE5578.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 800 1374
Size: 800 574
Acc65I G'GTAC_C
Cuts at: 0 953 1374
Size: 953 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1345 1374
Size: 979 366 29
Fragments arranged by size:
979 366 29
AfeI AGC'GCT
Cuts at: 0 394 898 1374
Size: 394 504 476
Fragments arranged by size:
504 476 394
AflIII A'CryG_T
Cuts at: 0 473 1374
Size: 473 901
AleI CACnn'nnGTG
Cuts at: 0 1072 1374
Size: 1072 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1229 1374
Size: 1229 145
AlwNI CAG_nnn'CTG
Cuts at: 0 259 384 507 761 1374
Size: 259 125 123 254 613
Fragments arranged by size:
613 259 254 125 123
ApaI G_GGCC'C
Cuts at: 0 45 1374
Size: 45 1329
ApaLI G'TGCA_C
Cuts at: 0 1250 1318 1374
Size: 1250 68 56
Fragments arranged by size:
1250 68 56
AvaI C'yCGr_G
Cuts at: 0 858 1184 1201 1374
Size: 858 326 17 173
Fragments arranged by size:
858 326 173 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 331 364 1374
Size: 331 33 1010
Fragments arranged by size:
1010 331 33
BamHI G'GATC_C
Cuts at: 0 732 1374
Size: 732 642
BanI G'GyrC_C
Cuts at: 0 27 631 800 953 1038 1130 1374
Size: 27 604 169 153 85 92 244
Fragments arranged by size:
604 244 169 153 92 85 27
BanII G_rGCy'C
Cuts at: 0 45 1374
Size: 45 1329
BbeI G_GCGC'C
Cuts at: 0 635 1134 1374
Size: 635 499 240
Fragments arranged by size:
635 499 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 1351 1374
Size: 1351 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 378 412 695 729 1374
Size: 283 34 61 34 283 34 645
Fragments arranged by size:
645 283 283 61 34 34 34
BclI T'GATC_A
Cuts at: 0 998 1374
Size: 998 376
BglI GCCn_nnn'nGGC
Cuts at: 0 49 1128 1374
Size: 49 1079 246
Fragments arranged by size:
1079 246 49
BglII A'GATC_T
Cuts at: 0 461 1374
Size: 461 913
Bme1580I G_kGCm'C
Cuts at: 0 45 1232 1254 1322 1374
Size: 45 1187 22 68 52
Fragments arranged by size:
1187 68 52 45 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 1374
Size: 334 1040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 1374
Size: 39 1335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1374
Size: 738 636
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 1315 1374
Size: 688 627 59
Fragments arranged by size:
688 627 59
BsaHI Gr'CG_yC
Cuts at: 0 632 967 1131 1374
Size: 632 335 164 243
Fragments arranged by size:
632 335 243 164
BsaWI w'CCGG_w
Cuts at: 0 481 1374
Size: 481 893
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 1374
Size: 463 30 881
Fragments arranged by size:
881 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 829 1286 1374
Size: 19 810 457 88
Fragments arranged by size:
810 457 88 19
BseYI C'CCAG_C
Cuts at: 0 1231 1374
Size: 1231 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 894 913 1071 1235 1374
Size: 153 741 19 158 164 139
Fragments arranged by size:
741 164 158 153 139 19
BsiEI CG_ry'CG
Cuts at: 0 405 1374
Size: 405 969
BsiHKAI G_wGCw'C
Cuts at: 0 11 434 1254 1322 1374
Size: 11 423 820 68 52
Fragments arranged by size:
820 423 68 52 11
BsmI GAATG_Cn'
Cuts at: 0 306 1007 1374
Size: 306 701 367
Fragments arranged by size:
701 367 306
BsmBI CGTCTCn'nnnn_
Cuts at: 0 861 1374
Size: 861 513
Bsp1286I G_dGCh'C
Cuts at: 0 11 45 434 1232 1254 1322 1374
Size: 11 34 389 798 22 68 52
Fragments arranged by size:
798 389 68 52 34 22 11
BspEI T'CCGG_A
Cuts at: 0 481 1374
Size: 481 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 800 1374
Size: 392 408 574
Fragments arranged by size:
574 408 392
BsrDI GCAATG_nn'
Cuts at: 0 83 1374
Size: 83 1291
BsrFI r'CCGG_y
Cuts at: 0 195 1374
Size: 195 1179
BsrGI T'GTAC_A
Cuts at: 0 446 593 1374
Size: 446 147 781
Fragments arranged by size:
781 446 147
BssSI C'ACGA_G
Cuts at: 0 661 1374
Size: 661 713
BstYI r'GATC_y
Cuts at: 0 461 732 816 1374
Size: 461 271 84 558
Fragments arranged by size:
558 461 271 84
Bsu36I CC'TnA_GG
Cuts at: 0 1043 1374
Size: 1043 331
BtgI C'CryG_G
Cuts at: 0 740 823 1099 1374
Size: 740 83 276 275
Fragments arranged by size:
740 276 275 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 519 1074 1374
Size: 519 555 300
Fragments arranged by size:
555 519 300
BtsI GCAGTG_nn'
Cuts at: 0 237 1374
Size: 237 1137
DraIII CAC_nnn'GTG
Cuts at: 0 243 666 807 1374
Size: 243 423 141 567
Fragments arranged by size:
567 423 243 141
DrdI GACnn_nn'nnGTC
Cuts at: 0 79 1374
Size: 79 1295
EaeI y'GGCC_r
Cuts at: 0 193 234 402 581 905 1374
Size: 193 41 168 179 324 469
Fragments arranged by size:
469 324 193 179 168 41
EagI C'GGCC_G
Cuts at: 0 402 1374
Size: 402 972
EarI CTCTTCn'nnn_
Cuts at: 0 217 313 691 907 1374
Size: 217 96 378 216 467
Fragments arranged by size:
467 378 217 216 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1374
Size: 403 971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 979 1345 1374
Size: 738 241 366 29
Fragments arranged by size:
738 366 241 29
EcoO109I rG'GnC_Cy
Cuts at: 0 41 51 203 1153 1374
Size: 41 10 152 950 221
Fragments arranged by size:
950 221 152 41 10
FspI TGC'GCA
Cuts at: 0 388 1374
Size: 388 986
HaeII r_GCGC'y
Cuts at: 0 266 396 635 900 1134 1374
Size: 266 130 239 265 234 240
Fragments arranged by size:
266 265 240 239 234 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 463 495 801 833 836 868
Size: 48 32 383 32 306 32 3 32
Cuts at: 868 1374
Size: 506
Fragments arranged by size:
506 383 306 48 32 32 32 32
3
KasI G'GCGC_C
Cuts at: 0 631 1130 1374
Size: 631 499 244
Fragments arranged by size:
631 499 244
KpnI G_GTAC'C
Cuts at: 0 957 1374
Size: 957 417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 1374
Size: 1109 265
MscI TGG'CCA
Cuts at: 0 236 1374
Size: 236 1138
MslI CAynn'nnrTG
Cuts at: 0 370 553 643 985 1072 1374
Size: 370 183 90 342 87 302
Fragments arranged by size:
370 342 302 183 90 87
MspA1I CmG'CkG
Cuts at: 0 358 1101 1209 1374
Size: 358 743 108 165
Fragments arranged by size:
743 358 165 108
NarI GG'CG_CC
Cuts at: 0 632 1131 1374
Size: 632 499 243
Fragments arranged by size:
632 499 243
NotI GC'GGCC_GC
Cuts at: 0 402 1374
Size: 402 972
NspI r_CATG'y
Cuts at: 0 477 1374
Size: 477 897
PciI A'CATG_T
Cuts at: 0 473 1374
Size: 473 901
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 666 1354 1374
Size: 243 423 688 20
Fragments arranged by size:
688 423 243 20
PshAI GACnn'nnGTC
Cuts at: 0 616 1374
Size: 616 758
PspOMI G'GGCC_C
Cuts at: 0 41 1374
Size: 41 1333
PvuII CAG'CTG
Cuts at: 0 1209 1374
Size: 1209 165
SacII CC_GC'GG
Cuts at: 0 1102 1374
Size: 1102 272
SapI GCTCTTCn'nnn_
Cuts at: 0 313 907 1374
Size: 313 594 467
Fragments arranged by size:
594 467 313
SexAI A'CCwGG_T
Cuts at: 0 881 1374
Size: 881 493
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 1374
Size: 49 1325
SfoI GGC'GCC
Cuts at: 0 633 1132 1374
Size: 633 499 242
Fragments arranged by size:
633 499 242
TatI w'GTAC_w
Cuts at: 0 446 593 1374
Size: 446 147 781
Fragments arranged by size:
781 446 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 808 840 1374
Size: 808 32 534
Fragments arranged by size:
808 534 32
Tth111I GACn'n_nGTC
Cuts at: 0 723 883 1374
Size: 723 160 491
Fragments arranged by size:
723 491 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 978 1374
Size: 465 513 396
Fragments arranged by size:
513 465 396
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BclI BglI BglII Bme1580I BmrI BplI
BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
FspI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NarI NotI NspI PciI PflMI PshAI PspOMI
PvuII SacII SapI SexAI SfiI SfoI TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BbvCI BciVI BfrBI BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsiWI BspHI
BsrBI BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII XbaI XhoI XmaI
XmnI ZraI