Restriction map for
pFN21AE5577
(Linear) (Six-base) MAPSORT of: KIEE5577.seq Check: 8889 from: 1 to: 1341
KIEE5577.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1142 1341
Size: 1142 199
AccI GT'mk_AC
Cuts at: 0 929 1341
Size: 929 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1341
Size: 522 819
AflIII A'CryG_T
Cuts at: 0 608 1341
Size: 608 733
AlwNI CAG_nnn'CTG
Cuts at: 0 252 482 1341
Size: 252 230 859
Fragments arranged by size:
859 252 230
AseI AT'TA_AT
Cuts at: 0 299 1341
Size: 299 1042
AvrII C'CTAG_G
Cuts at: 0 1030 1341
Size: 1030 311
BanI G'GyrC_C
Cuts at: 0 765 910 1341
Size: 765 145 431
Fragments arranged by size:
765 431 145
BanII G_rGCy'C
Cuts at: 0 650 1210 1341
Size: 650 560 131
Fragments arranged by size:
650 560 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 46 123 864 972 1341
Size: 46 77 741 108 369
Fragments arranged by size:
741 369 108 77 46
BbvCI CC'TCA_GC
Cuts at: 0 799 1341
Size: 799 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 848 1341
Size: 814 34 493
Fragments arranged by size:
814 493 34
BglI GCCn_nnn'nGGC
Cuts at: 0 21 1035 1341
Size: 21 1014 306
Fragments arranged by size:
1014 306 21
Bme1580I G_kGCm'C
Cuts at: 0 33 770 837 1341
Size: 33 737 67 504
Fragments arranged by size:
737 504 67 33
BmgBI CAC'GTC
Cuts at: 0 611 1341
Size: 611 730
BmrI ACTGGGnnnn_n'
Cuts at: 0 764 1341
Size: 764 577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 991 1341
Size: 229 762 350
Fragments arranged by size:
762 350 229
Bpu10I CC'TnA_GC
Cuts at: 0 96 799 1209 1341
Size: 96 703 410 132
Fragments arranged by size:
703 410 132 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 421 967 1123 1183 1341
Size: 421 546 156 60 158
Fragments arranged by size:
546 421 158 156 60
BsaAI yAC'GTr
Cuts at: 0 1002 1341
Size: 1002 339
BsaBI GATnn'nnATC
Cuts at: 0 398 1341
Size: 398 943
BsaHI Gr'CG_yC
Cuts at: 0 406 1341
Size: 406 935
BsaWI w'CCGG_w
Cuts at: 0 412 1341
Size: 412 929
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1065 1095 1341
Size: 1065 30 246
Fragments arranged by size:
1065 246 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1296 1341
Size: 25 1271 45
Fragments arranged by size:
1271 45 25
BseYI C'CCAG_C
Cuts at: 0 573 858 1013 1176 1226 1341
Size: 573 285 155 163 50 115
Fragments arranged by size:
573 285 163 155 115 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1044 1267 1341
Size: 982 62 223 74
Fragments arranged by size:
982 223 74 62
BsiHKAI G_wGCw'C
Cuts at: 0 650 1341
Size: 650 691
BsmBI CGTCTCn'nnnn_
Cuts at: 0 617 1341
Size: 617 724
Bsp1286I G_dGCh'C
Cuts at: 0 33 650 770 837 1210 1341
Size: 33 617 120 67 373 131
Fragments arranged by size:
617 373 131 120 67 33
BspHI T'CATG_A
Cuts at: 0 881 1341
Size: 881 460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 818 1001 1142 1298 1341
Size: 767 51 183 141 156 43
Fragments arranged by size:
767 183 156 141 51 43
BstEII G'GTnAC_C
Cuts at: 0 471 1341
Size: 471 870
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 1138 1341
Size: 396 742 203
Fragments arranged by size:
742 396 203
BtgI C'CryG_G
Cuts at: 0 389 683 1341
Size: 389 294 658
Fragments arranged by size:
658 389 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1276 1341
Size: 1276 65
BtsI GCAGTG_nn'
Cuts at: 0 146 419 441 1056 1085 1341
Size: 146 273 22 615 29 256
Fragments arranged by size:
615 273 256 146 29 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 1341
Size: 127 1214
EaeI y'GGCC_r
Cuts at: 0 722 1004 1341
Size: 722 282 337
Fragments arranged by size:
722 337 282
EarI CTCTTCn'nnn_
Cuts at: 0 63 1282 1300 1341
Size: 63 1219 18 41
Fragments arranged by size:
1219 63 41 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1270 1341
Size: 1270 71
EcoICRI GAG'CTC
Cuts at: 0 648 1341
Size: 648 693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 522 991 1341
Size: 229 293 469 350
Fragments arranged by size:
469 350 293 229
EcoO109I rG'GnC_Cy
Cuts at: 0 1110 1180 1341
Size: 1110 70 161
Fragments arranged by size:
1110 161 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 813 845 1065 1097 1192 1224 1341
Size: 813 32 220 32 95 32 117
Fragments arranged by size:
813 220 117 95 32 32 32
HincII GTy'rAC
Cuts at: 0 120 198 1341
Size: 120 78 1143
Fragments arranged by size:
1143 120 78
MfeI C'AATT_G
Cuts at: 0 90 1341
Size: 90 1251
MscI TGG'CCA
Cuts at: 0 724 1006 1341
Size: 724 282 335
Fragments arranged by size:
724 335 282
MslI CAynn'nnrTG
Cuts at: 0 264 394 1341
Size: 264 130 947
Fragments arranged by size:
947 264 130
MspA1I CmG'CkG
Cuts at: 0 249 1341
Size: 249 1092
NcoI C'CATG_G
Cuts at: 0 389 683 1341
Size: 389 294 658
Fragments arranged by size:
658 389 294
NspI r_CATG'y
Cuts at: 0 570 1341
Size: 570 771
PasI CC'CwG_GG
Cuts at: 0 633 1069 1341
Size: 633 436 272
Fragments arranged by size:
633 436 272
PflMI CCAn_nnn'nTGG
Cuts at: 0 1233 1263 1341
Size: 1233 30 78
Fragments arranged by size:
1233 78 30
PmlI CAC'GTG
Cuts at: 0 1002 1341
Size: 1002 339
PpuMI rG'GwC_Cy
Cuts at: 0 1180 1341
Size: 1180 161
PvuII CAG'CTG
Cuts at: 0 249 1341
Size: 249 1092
SacI G_AGCT'C
Cuts at: 0 650 1341
Size: 650 691
SanDI GG'GwC_CC
Cuts at: 0 1180 1341
Size: 1180 161
SapI GCTCTTCn'nnn_
Cuts at: 0 1282 1300 1341
Size: 1282 18 41
Fragments arranged by size:
1282 41 18
SfcI C'TryA_G
Cuts at: 0 177 1341
Size: 177 1164
SphI G_CATG'C
Cuts at: 0 570 1341
Size: 570 771
StuI AGG'CCT
Cuts at: 0 157 1341
Size: 157 1184
StyI C'CwwG_G
Cuts at: 0 389 683 1030 1341
Size: 389 294 347 311
Fragments arranged by size:
389 347 311 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 1038 1144 1207 1233 1341
Size: 1027 11 106 63 26 108
Fragments arranged by size:
1027 108 106 63 26 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1341
Size: 27 1314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 228 777 1341
Size: 228 549 564
Fragments arranged by size:
564 549 228
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AseI AvrII BanI
BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BstEII BstXI BtgI BtgZI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I Hin4I HincII MfeI MscI
MslI MspA1I NcoI NspI PasI PflMI PmlI PpuMI
PvuII SacI SanDI SapI SfcI SphI StuI StyI
TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI
BmtI BplI BpuEI BsiEI BsiWI BsmI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI