Restriction map for
pFN21AE5576
(Linear) (Six-base) MAPSORT of: KIEE5576.seq Check: 5804 from: 1 to: 1170
KIEE5576.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AccI GT'mk_AC
Cuts at: 0 773 917 989 1061 1170
Size: 773 144 72 72 109
Fragments arranged by size:
773 144 109 72 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1170
Size: 343 827
AleI CACnn'nnGTG
Cuts at: 0 1083 1170
Size: 1083 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 1170
Size: 127 1043
ApaI G_GGCC'C
Cuts at: 0 334 1170
Size: 334 836
ApoI r'AATT_y
Cuts at: 0 732 876 948 1020 1170
Size: 732 144 72 72 150
Fragments arranged by size:
732 150 144 72 72
BanII G_rGCy'C
Cuts at: 0 334 1170
Size: 334 836
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 1170
Size: 53 1117
Bme1580I G_kGCm'C
Cuts at: 0 334 1090 1170
Size: 334 756 80
Fragments arranged by size:
756 334 80
BmgBI CAC'GTC
Cuts at: 0 270 379 1170
Size: 270 109 791
Fragments arranged by size:
791 270 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 1170
Size: 51 1119
BmtI G_CTAG'C
Cuts at: 0 80 1170
Size: 80 1090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 1170
Size: 24 1146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 389 392 1170
Size: 389 3 778
Fragments arranged by size:
778 389 3
BseYI C'CCAG_C
Cuts at: 0 86 327 1170
Size: 86 241 843
Fragments arranged by size:
843 241 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1170
Size: 391 779
BsiHKAI G_wGCw'C
Cuts at: 0 363 1170
Size: 363 807
Bsp1286I G_dGCh'C
Cuts at: 0 334 363 1090 1170
Size: 334 29 727 80
Fragments arranged by size:
727 334 80 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 1170
Size: 461 709
BstAPI GCAn_nnn'nTGC
Cuts at: 0 721 865 937 1009 1170
Size: 721 144 72 72 161
Fragments arranged by size:
721 161 144 72 72
BstEII G'GTnAC_C
Cuts at: 0 603 963 1035 1170
Size: 603 360 72 135
Fragments arranged by size:
603 360 135 72
BstZ17I GTA'TAC
Cuts at: 0 774 918 990 1062 1170
Size: 774 144 72 72 108
Fragments arranged by size:
774 144 108 72 72
BtgI C'CryG_G
Cuts at: 0 309 1170
Size: 309 861
BtsI GCAGTG_nn'
Cuts at: 0 677 821 1090 1170
Size: 677 144 269 80
Fragments arranged by size:
677 269 144 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 365 400 1170
Size: 365 35 770
Fragments arranged by size:
770 365 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 274 1170
Size: 274 896
EarI CTCTTCn'nnn_
Cuts at: 0 1158 1170
Size: 1158 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 444 1170
Size: 444 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 343 1170
Size: 24 319 827
Fragments arranged by size:
827 319 24
EcoO109I rG'GnC_Cy
Cuts at: 0 331 1170
Size: 331 839
EcoRV GAT'ATC
Cuts at: 0 616 688 760 832 904 976 1048 1170
Size: 616 72 72 72 72 72 72 122
Fragments arranged by size:
616 122 72 72 72 72 72 72
MslI CAynn'nnrTG
Cuts at: 0 308 1083 1131 1170
Size: 308 775 48 39
Fragments arranged by size:
775 308 48 39
MspA1I CmG'CkG
Cuts at: 0 70 1170
Size: 70 1100
NcoI C'CATG_G
Cuts at: 0 309 1170
Size: 309 861
NheI G'CTAG_C
Cuts at: 0 76 1170
Size: 76 1094
PspOMI G'GGCC_C
Cuts at: 0 330 1170
Size: 330 840
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 174 206 1170
Size: 174 32 964
Fragments arranged by size:
964 174 32
PstI C_TGCA'G
Cuts at: 0 75 458 1170
Size: 75 383 712
Fragments arranged by size:
712 383 75
PvuII CAG'CTG
Cuts at: 0 70 1170
Size: 70 1100
SexAI A'CCwGG_T
Cuts at: 0 522 1170
Size: 522 648
SfcI C'TryA_G
Cuts at: 0 71 454 1170
Size: 71 383 716
Fragments arranged by size:
716 383 71
StyI C'CwwG_G
Cuts at: 0 81 309 1170
Size: 81 228 861
Fragments arranged by size:
861 228 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 78 1135 1170
Size: 70 8 1057 35
Fragments arranged by size:
1057 70 35 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 1170
Size: 459 32 679
Fragments arranged by size:
679 459 32
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApoI BanII BbsI
Bme1580I BmgBI BmrI BmtI BpmI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BstAPI BstEII BstZ17I BtgI BtsI
CspCI DrdI EarI EciI Eco57MI EcoO109I EcoRV MslI
MspA1I NcoI NheI PspOMI PsrI PstI PvuII SexAI
SfcI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstYI Bsu36I BtgZI ClaI
DraI DraIII EaeI EagI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI