Restriction map for
pFN21AE5575
(Linear) (Six-base) MAPSORT of: KIEE5575.seq Check: 4389 from: 1 to: 1386
KIEE5575.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 275 1386
Size: 275 1111
Acc65I G'GTAC_C
Cuts at: 0 788 1386
Size: 788 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1386
Size: 136 1250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1173 1386
Size: 1173 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 1386
Size: 682 32 672
Fragments arranged by size:
682 672 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 240 687 774 1027 1045 1386
Size: 60 180 447 87 253 18 341
Fragments arranged by size:
447 341 253 180 87 60 18
ApaI G_GGCC'C
Cuts at: 0 70 502 1386
Size: 70 432 884
Fragments arranged by size:
884 432 70
ApaLI G'TGCA_C
Cuts at: 0 508 1386
Size: 508 878
AseI AT'TA_AT
Cuts at: 0 1300 1386
Size: 1300 86
AvaI C'yCGr_G
Cuts at: 0 166 751 1386
Size: 166 585 635
Fragments arranged by size:
635 585 166
BanI G'GyrC_C
Cuts at: 0 788 926 1180 1386
Size: 788 138 254 206
Fragments arranged by size:
788 254 206 138
BanII G_rGCy'C
Cuts at: 0 70 502 761 1386
Size: 70 432 259 625
Fragments arranged by size:
625 432 259 70
BbeI G_GCGC'C
Cuts at: 0 930 1386
Size: 930 456
BbsI GAAGACnn'nnnn_
Cuts at: 0 385 1386
Size: 385 1001
Bme1580I G_kGCm'C
Cuts at: 0 70 502 512 1386
Size: 70 432 10 874
Fragments arranged by size:
874 432 70 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1366 1386
Size: 1366 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 963 1386
Size: 963 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 374 720 998 1386
Size: 127 247 346 278 388
Fragments arranged by size:
388 346 278 247 127
BsaHI Gr'CG_yC
Cuts at: 0 927 1386
Size: 927 459
BsaWI w'CCGG_w
Cuts at: 0 272 1386
Size: 272 1114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 1045 1075 1386
Size: 694 30 321 30 311
Fragments arranged by size:
694 321 311 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 21 339 769 1386
Size: 19 2 318 430 617
Fragments arranged by size:
617 430 318 19 2
BseYI C'CCAG_C
Cuts at: 0 640 767 1386
Size: 640 127 619
Fragments arranged by size:
640 619 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1386
Size: 441 945
BsiEI CG_ry'CG
Cuts at: 0 651 1386
Size: 651 735
BsiHKAI G_wGCw'C
Cuts at: 0 176 512 761 1262 1386
Size: 176 336 249 501 124
Fragments arranged by size:
501 336 249 176 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 1386
Size: 315 1071
Bsp1286I G_dGCh'C
Cuts at: 0 70 176 502 512 761 1262 1386
Size: 70 106 326 10 249 501 124
Fragments arranged by size:
501 326 249 124 106 70 10
BspHI T'CATG_A
Cuts at: 0 1265 1386
Size: 1265 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 1217 1386
Size: 275 942 169
Fragments arranged by size:
942 275 169
BsrBI CCG'CTC
Cuts at: 0 601 1386
Size: 601 785
BsrDI GCAATG_nn'
Cuts at: 0 1145 1386
Size: 1145 241
BsrFI r'CCGG_y
Cuts at: 0 646 1090 1386
Size: 646 444 296
Fragments arranged by size:
646 444 296
BsrGI T'GTAC_A
Cuts at: 0 242 1386
Size: 242 1144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1027 1110 1386
Size: 1027 83 276
Fragments arranged by size:
1027 276 83
BstEII G'GTnAC_C
Cuts at: 0 1086 1386
Size: 1086 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 444 1386
Size: 204 240 942
Fragments arranged by size:
942 240 204
Bsu36I CC'TnA_GG
Cuts at: 0 33 962 1386
Size: 33 929 424
Fragments arranged by size:
929 424 33
BtgI C'CryG_G
Cuts at: 0 197 796 1064 1213 1386
Size: 197 599 268 149 173
Fragments arranged by size:
599 268 197 173 149
BtsI GCAGTG_nn'
Cuts at: 0 1108 1386
Size: 1108 278
DraIII CAC_nnn'GTG
Cuts at: 0 1248 1386
Size: 1248 138
EaeI y'GGCC_r
Cuts at: 0 605 648 860 1092 1289 1386
Size: 605 43 212 232 197 97
Fragments arranged by size:
605 232 212 197 97 43
EagI C'GGCC_G
Cuts at: 0 648 1386
Size: 648 738
EarI CTCTTCn'nnn_
Cuts at: 0 133 324 487 1386
Size: 133 191 163 899
Fragments arranged by size:
899 191 163 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 121 1386
Size: 73 48 1265
Fragments arranged by size:
1265 73 48
EcoICRI GAG'CTC
Cuts at: 0 759 1386
Size: 759 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 136 374 720 998 1386
Size: 127 9 238 346 278 388
Fragments arranged by size:
388 346 278 238 127 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 1386
Size: 139 1247
EcoO109I rG'GnC_Cy
Cuts at: 0 66 67 499 815 1386
Size: 66 1 432 316 571
Fragments arranged by size:
571 432 316 66 1
EcoRV GAT'ATC
Cuts at: 0 420 1386
Size: 420 966
FspI TGC'GCA
Cuts at: 0 550 1386
Size: 550 836
FspAI rTGC'GCAy
Cuts at: 0 550 1386
Size: 550 836
HaeII r_GCGC'y
Cuts at: 0 647 721 930 1072 1386
Size: 647 74 209 142 314
Fragments arranged by size:
647 314 209 142 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 905 937 975 1007 1386
Size: 694 32 179 32 38 32 379
Fragments arranged by size:
694 379 179 38 32 32 32
KasI G'GCGC_C
Cuts at: 0 926 1386
Size: 926 460
KpnI G_GTAC'C
Cuts at: 0 792 1386
Size: 792 594
MscI TGG'CCA
Cuts at: 0 607 862 1291 1386
Size: 607 255 429 95
Fragments arranged by size:
607 429 255 95
MslI CAynn'nnrTG
Cuts at: 0 874 1300 1386
Size: 874 426 86
Fragments arranged by size:
874 426 86
MspA1I CmG'CkG
Cuts at: 0 191 798 1386
Size: 191 607 588
Fragments arranged by size:
607 588 191
NaeI GCC'GGC
Cuts at: 0 648 1386
Size: 648 738
NarI GG'CG_CC
Cuts at: 0 927 1386
Size: 927 459
NcoI C'CATG_G
Cuts at: 0 197 1386
Size: 197 1189
NgoMIV G'CCGG_C
Cuts at: 0 646 1386
Size: 646 740
NspI r_CATG'y
Cuts at: 0 1108 1386
Size: 1108 278
PfoI T'CCnGG_A
Cuts at: 0 283 368 1386
Size: 283 85 1018
Fragments arranged by size:
1018 283 85
PpuMI rG'GwC_Cy
Cuts at: 0 815 1386
Size: 815 571
PspOMI G'GGCC_C
Cuts at: 0 66 498 1386
Size: 66 432 888
Fragments arranged by size:
888 432 66
PstI C_TGCA'G
Cuts at: 0 56 1032 1386
Size: 56 976 354
Fragments arranged by size:
976 354 56
PvuII CAG'CTG
Cuts at: 0 191 1386
Size: 191 1195
SacI G_AGCT'C
Cuts at: 0 761 1386
Size: 761 625
SacII CC_GC'GG
Cuts at: 0 799 1386
Size: 799 587
SanDI GG'GwC_CC
Cuts at: 0 815 1386
Size: 815 571
SapI GCTCTTCn'nnn_
Cuts at: 0 133 1386
Size: 133 1253
SfcI C'TryA_G
Cuts at: 0 52 1028 1386
Size: 52 976 358
Fragments arranged by size:
976 358 52
SfoI GGC'GCC
Cuts at: 0 928 1386
Size: 928 458
SphI G_CATG'C
Cuts at: 0 1108 1386
Size: 1108 278
StyI C'CwwG_G
Cuts at: 0 197 611 1332 1386
Size: 197 414 721 54
Fragments arranged by size:
721 414 197 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 654 1386
Size: 654 732
TatI w'GTAC_w
Cuts at: 0 242 593 1320 1386
Size: 242 351 727 66
Fragments arranged by size:
727 351 242 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1386
Size: 1045 32 309
Fragments arranged by size:
1045 309 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1386
Size: 1071 315
XmnI GAAnn'nnTTC
Cuts at: 0 127 1386
Size: 127 1259
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI ApaI ApaLI
AseI AvaI BanI BanII BbeI BbsI Bme1580I BmrI
BplI BpmI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI Bsu36I BtgI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FspI FspAI HaeII Hin4I KasI KpnI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI SfcI SfoI SphI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApoI AscI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsiWI BsmI
BspEI BssHII BssSI BstBI BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EcoRI FalI FseI HincII HindIII
HpaI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI