Restriction map for
pFN21AE5571
(Linear) (Six-base) MAPSORT of: KIEE5571.seq Check: 3220 from: 1 to: 1359
KIEE5571.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AccI GT'mk_AC
Cuts at: 0 229 1359
Size: 229 1130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 390 973 1129 1359
Size: 188 202 583 156 230
Fragments arranged by size:
583 230 202 188 156
ApaI G_GGCC'C
Cuts at: 0 206 1359
Size: 206 1153
ApoI r'AATT_y
Cuts at: 0 1265 1359
Size: 1265 94
AseI AT'TA_AT
Cuts at: 0 791 1359
Size: 791 568
AvrII C'CTAG_G
Cuts at: 0 51 1359
Size: 51 1308
BamHI G'GATC_C
Cuts at: 0 101 1359
Size: 101 1258
BanI G'GyrC_C
Cuts at: 0 1237 1359
Size: 1237 122
BanII G_rGCy'C
Cuts at: 0 206 1359
Size: 206 1153
BbsI GAAGACnn'nnnn_
Cuts at: 0 933 1359
Size: 933 426
Bme1580I G_kGCm'C
Cuts at: 0 206 870 1359
Size: 206 664 489
Fragments arranged by size:
664 489 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 1270 1317 1359
Size: 1270 47 42
Fragments arranged by size:
1270 47 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1359
Size: 601 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1359
Size: 499 860
BsaAI yAC'GTr
Cuts at: 0 560 1359
Size: 560 799
BsaHI Gr'CG_yC
Cuts at: 0 4 337 1359
Size: 4 333 1022
Fragments arranged by size:
1022 333 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 211 241 1359
Size: 146 30 35 30 1118
Fragments arranged by size:
1118 146 35 30 30
BseYI C'CCAG_C
Cuts at: 0 807 1359
Size: 807 552
BsiEI CG_ry'CG
Cuts at: 0 145 1359
Size: 145 1214
BsiHKAI G_wGCw'C
Cuts at: 0 1172 1359
Size: 1172 187
BsmI GAATG_Cn'
Cuts at: 0 934 1359
Size: 934 425
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 1359
Size: 14 1345
Bsp1286I G_dGCh'C
Cuts at: 0 206 870 1172 1359
Size: 206 664 302 187
Fragments arranged by size:
664 302 206 187
BspHI T'CATG_A
Cuts at: 0 728 1359
Size: 728 631
BsrDI GCAATG_nn'
Cuts at: 0 371 1359
Size: 371 988
BssSI C'ACGA_G
Cuts at: 0 1173 1359
Size: 1173 186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 879 1021 1359
Size: 879 142 338
Fragments arranged by size:
879 338 142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 906 1359
Size: 477 429 453
Fragments arranged by size:
477 453 429
BstYI r'GATC_y
Cuts at: 0 101 1359
Size: 101 1258
BstZ17I GTA'TAC
Cuts at: 0 230 1359
Size: 230 1129
BtgI C'CryG_G
Cuts at: 0 905 1359
Size: 905 454
BtsI GCAGTG_nn'
Cuts at: 0 899 1356 1359
Size: 899 457 3
Fragments arranged by size:
899 457 3
EaeI y'GGCC_r
Cuts at: 0 142 1359
Size: 142 1217
EagI C'GGCC_G
Cuts at: 0 142 1359
Size: 142 1217
EarI CTCTTCn'nnn_
Cuts at: 0 409 1276 1359
Size: 409 867 83
Fragments arranged by size:
867 409 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 1359
Size: 238 1121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 390 601 973 1129 1359
Size: 188 202 211 372 156 230
Fragments arranged by size:
372 230 211 202 188 156
EcoRI G'AATT_C
Cuts at: 0 1265 1359
Size: 1265 94
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 592 1139 1359
Size: 592 547 220
Fragments arranged by size:
592 547 220
HaeII r_GCGC'y
Cuts at: 0 1183 1359
Size: 1183 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 146 178 288 320 729 761 1110 1142
Size: 146 32 110 32 409 32 349 32
Cuts at: 1142 1169 1201 1359
Size: 27 32 158
Fragments arranged by size:
409 349 158 146 110 32 32 32
32 32 27
HincII GTy'rAC
Cuts at: 0 483 972 1359
Size: 483 489 387
Fragments arranged by size:
489 483 387
HpaI GTT'AAC
Cuts at: 0 483 1359
Size: 483 876
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1359
Size: 79 1280
MslI CAynn'nnrTG
Cuts at: 0 187 904 1359
Size: 187 717 455
Fragments arranged by size:
717 455 187
MspA1I CmG'CkG
Cuts at: 0 967 1359
Size: 967 392
NcoI C'CATG_G
Cuts at: 0 905 1359
Size: 905 454
NdeI CA'TA_TG
Cuts at: 0 894 1359
Size: 894 465
NspI r_CATG'y
Cuts at: 0 505 1359
Size: 505 854
PflMI CCAn_nnn'nTGG
Cuts at: 0 911 1231 1359
Size: 911 320 128
Fragments arranged by size:
911 320 128
PfoI T'CCnGG_A
Cuts at: 0 104 1359
Size: 104 1255
PsiI TTA'TAA
Cuts at: 0 685 1359
Size: 685 674
PspOMI G'GGCC_C
Cuts at: 0 202 1359
Size: 202 1157
PvuII CAG'CTG
Cuts at: 0 967 1359
Size: 967 392
SmlI C'TyrA_G
Cuts at: 0 514 1359
Size: 514 845
SphI G_CATG'C
Cuts at: 0 505 1359
Size: 505 854
StyI C'CwwG_G
Cuts at: 0 51 905 1359
Size: 51 854 454
Fragments arranged by size:
854 454 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1359
Size: 1047 312
TatI w'GTAC_w
Cuts at: 0 1304 1359
Size: 1304 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1332 1359
Size: 1332 27
Enzymes that do cut:
AccI AcuI ApaI ApoI AseI AvrII BamHI BanI
BanII BbsI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI
BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrDI BssSI BstAPI BstXI BstYI BstZ17I BtgI BtsI
EaeI EagI EarI EciI Eco57MI EcoRI FalI HaeII
Hin4I HincII HpaI MmeI MslI MspA1I NcoI NdeI
NspI PflMI PfoI PsiI PspOMI PvuII SmlI SphI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI
AvaI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BseRI BsgI BsiWI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstBI BstEII Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI