Restriction map for
pFN21AE5569
(Linear) (Six-base) MAPSORT of: KIEE5569.seq Check: 8746 from: 1 to: 1338
KIEE5569.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AatII G_ACGT'C
Cuts at: 0 518 1338
Size: 518 820
Acc65I G'GTAC_C
Cuts at: 0 1152 1338
Size: 1152 186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 136 184 349 502 603 817 1338
Size: 91 45 48 165 153 101 214 521
Fragments arranged by size:
521 214 165 153 101 91 48 45
AfeI AGC'GCT
Cuts at: 0 1319 1338
Size: 1319 19
AflIII A'CryG_T
Cuts at: 0 26 1338
Size: 26 1312
AleI CACnn'nnGTG
Cuts at: 0 788 1338
Size: 788 550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1105 1338
Size: 1105 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1338
Size: 1096 32 210
Fragments arranged by size:
1096 210 32
AlwNI CAG_nnn'CTG
Cuts at: 0 840 1338
Size: 840 498
ApaLI G'TGCA_C
Cuts at: 0 19 1338
Size: 19 1319
AvaI C'yCGr_G
Cuts at: 0 1156 1188 1304 1338
Size: 1156 32 116 34
Fragments arranged by size:
1156 116 34 32
BanI G'GyrC_C
Cuts at: 0 1152 1234 1338
Size: 1152 82 104
Fragments arranged by size:
1152 104 82
BanII G_rGCy'C
Cuts at: 0 47 496 1165 1338
Size: 47 449 669 173
Fragments arranged by size:
669 449 173 47
BbeI G_GCGC'C
Cuts at: 0 1238 1338
Size: 1238 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 1338
Size: 456 34 848
Fragments arranged by size:
848 456 34
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1338
Size: 290 1048
BlpI GC'TnA_GC
Cuts at: 0 307 1138 1338
Size: 307 831 200
Fragments arranged by size:
831 307 200
Bme1580I G_kGCm'C
Cuts at: 0 23 635 1207 1338
Size: 23 612 572 131
Fragments arranged by size:
612 572 131 23
BmgBI CAC'GTC
Cuts at: 0 29 1338
Size: 29 1309
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 387 1338
Size: 153 234 951
Fragments arranged by size:
951 234 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 510 1338
Size: 510 828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 838 862 955 1042 1093 1338
Size: 472 366 24 93 87 51 245
Fragments arranged by size:
472 366 245 93 87 51 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 1338
Size: 409 929
BsaBI GATnn'nnATC
Cuts at: 0 91 1338
Size: 91 1247
BsaHI Gr'CG_yC
Cuts at: 0 398 515 1227 1235 1338
Size: 398 117 712 8 103
Fragments arranged by size:
712 398 117 103 8
BsaWI w'CCGG_w
Cuts at: 0 551 1338
Size: 551 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 367 397 1338
Size: 175 30 162 30 941
Fragments arranged by size:
941 175 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 901 1054 1090 1338
Size: 604 297 153 36 248
Fragments arranged by size:
604 297 248 153 36
BseYI C'CCAG_C
Cuts at: 0 505 1338
Size: 505 833
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 676 1338
Size: 196 480 662
Fragments arranged by size:
662 480 196
BsiEI CG_ry'CG
Cuts at: 0 344 436 1338
Size: 344 92 902
Fragments arranged by size:
902 344 92
BsiHKAI G_wGCw'C
Cuts at: 0 23 47 217 1338
Size: 23 24 170 1121
Fragments arranged by size:
1121 170 24 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 47 217 496 635 1165 1207 1338
Size: 23 24 170 279 139 530 42 131
Fragments arranged by size:
530 279 170 139 131 42 24 23
BspEI T'CCGG_A
Cuts at: 0 551 1338
Size: 551 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1115 1338
Size: 1115 223
BsrBI CCG'CTC
Cuts at: 0 432 901 1338
Size: 432 469 437
Fragments arranged by size:
469 437 432
BsrFI r'CCGG_y
Cuts at: 0 1248 1338
Size: 1248 90
BsrGI T'GTAC_A
Cuts at: 0 719 1338
Size: 719 619
BssHII G'CGCG_C
Cuts at: 0 336 1338
Size: 336 1002
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 450 1338
Size: 99 351 888
Fragments arranged by size:
888 351 99
BstYI r'GATC_y
Cuts at: 0 548 1338
Size: 548 790
BtgI C'CryG_G
Cuts at: 0 38 218 743 1338
Size: 38 180 525 595
Fragments arranged by size:
595 525 180 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1110 1338
Size: 1110 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 1338
Size: 520 818
EaeI y'GGCC_r
Cuts at: 0 341 433 1338
Size: 341 92 905
Fragments arranged by size:
905 341 92
EagI C'GGCC_G
Cuts at: 0 341 433 1338
Size: 341 92 905
Fragments arranged by size:
905 341 92
EarI CTCTTCn'nnn_
Cuts at: 0 923 950 1338
Size: 923 27 388
Fragments arranged by size:
923 388 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1051 1338
Size: 1051 287
EcoICRI GAG'CTC
Cuts at: 0 45 1338
Size: 45 1293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 136 184 349 472 502 603 817
Size: 91 45 48 165 123 30 101 214
Cuts at: 817 838 862 955 1042 1093 1338
Size: 21 24 93 87 51 245
Fragments arranged by size:
245 214 165 123 101 93 91 87
51 48 45 30 24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1338
Size: 523 815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 375 979 1338
Size: 375 604 359
Fragments arranged by size:
604 375 359
HaeII r_GCGC'y
Cuts at: 0 698 1238 1321 1338
Size: 698 540 83 17
Fragments arranged by size:
698 540 83 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 1281 1313 1338
Size: 367 32 882 32 25
Fragments arranged by size:
882 367 32 32 25
HindIII A'AGCT_T
Cuts at: 0 829 961 1338
Size: 829 132 377
Fragments arranged by size:
829 377 132
KasI G'GCGC_C
Cuts at: 0 1234 1338
Size: 1234 104
KpnI G_GTAC'C
Cuts at: 0 1156 1338
Size: 1156 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 344 1338
Size: 164 180 994
Fragments arranged by size:
994 180 164
MslI CAynn'nnrTG
Cuts at: 0 85 97 568 788 1338
Size: 85 12 471 220 550
Fragments arranged by size:
550 471 220 85 12
MspA1I CmG'CkG
Cuts at: 0 7 114 311 816 1053 1258 1338
Size: 7 107 197 505 237 205 80
Fragments arranged by size:
505 237 205 197 107 80 7
NaeI GCC'GGC
Cuts at: 0 1250 1338
Size: 1250 88
NarI GG'CG_CC
Cuts at: 0 1235 1338
Size: 1235 103
NcoI C'CATG_G
Cuts at: 0 218 1338
Size: 218 1120
NgoMIV G'CCGG_C
Cuts at: 0 1248 1338
Size: 1248 90
PstI C_TGCA'G
Cuts at: 0 1034 1058 1112 1174 1257 1338
Size: 1034 24 54 62 83 81
Fragments arranged by size:
1034 83 81 62 54 24
PvuII CAG'CTG
Cuts at: 0 114 816 1053 1258 1338
Size: 114 702 237 205 80
Fragments arranged by size:
702 237 205 114 80
SacI G_AGCT'C
Cuts at: 0 47 1338
Size: 47 1291
SapI GCTCTTCn'nnn_
Cuts at: 0 923 1338
Size: 923 415
SfcI C'TryA_G
Cuts at: 0 1030 1054 1108 1170 1253 1259 1338
Size: 1030 24 54 62 83 6 79
Fragments arranged by size:
1030 83 79 62 54 24 6
SfoI GGC'GCC
Cuts at: 0 1236 1338
Size: 1236 102
SmaI CCC'GGG
Cuts at: 0 1158 1190 1306 1338
Size: 1158 32 116 32
Fragments arranged by size:
1158 116 32 32
SrfI GCCC'GGGC
Cuts at: 0 1190 1338
Size: 1190 148
StuI AGG'CCT
Cuts at: 0 649 1338
Size: 649 689
StyI C'CwwG_G
Cuts at: 0 218 401 1148 1338
Size: 218 183 747 190
Fragments arranged by size:
747 218 190 183
TatI w'GTAC_w
Cuts at: 0 668 719 1338
Size: 668 51 619
Fragments arranged by size:
668 619 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 1338
Size: 175 32 1131
Fragments arranged by size:
1131 175 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 1338
Size: 216 1122
XmaI C'CCGG_G
Cuts at: 0 1156 1188 1304 1338
Size: 1156 32 116 34
Fragments arranged by size:
1156 116 34 32
ZraI GAC'GTC
Cuts at: 0 516 1338
Size: 516 822
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaLI AvaI BanI BanII BbeI BcgI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BstXI
BstYI BtgI BtgZI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI FalI HaeII Hin4I HindIII KasI
KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
PstI PvuII SacI SapI SfcI SfoI SmaI SrfI
StuI StyI TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AgeI AhdI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BclI BfrBI BglII BmtI Bpu10I BpuEI BsaAI
BsiWI BsmI BsmBI BspHI BsrDI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HpaI
MfeI MluI MscI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI
TaqII Tth111I XbaI XhoI XmnI