Restriction map for
pFN21AE5567
(Linear) (Six-base) MAPSORT of: KIEE5567.seq Check: 1821 from: 1 to: 1299
KIEE5567.orf
With 182 enzymes: SgfI *
January 29, 2014 12:06 ..
AccI GT'mk_AC
Cuts at: 0 1228 1299
Size: 1228 71
Acc65I G'GTAC_C
Cuts at: 0 1027 1299
Size: 1027 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 144 189 1199 1299
Size: 71 73 45 1010 100
Fragments arranged by size:
1010 100 73 71 45
AfeI AGC'GCT
Cuts at: 0 157 1299
Size: 157 1142
AlwNI CAG_nnn'CTG
Cuts at: 0 49 270 297 1299
Size: 49 221 27 1002
Fragments arranged by size:
1002 221 49 27
ApaI G_GGCC'C
Cuts at: 0 1241 1257 1299
Size: 1241 16 42
Fragments arranged by size:
1241 42 16
ApoI r'AATT_y
Cuts at: 0 678 1299
Size: 678 621
AvaI C'yCGr_G
Cuts at: 0 1126 1299
Size: 1126 173
AvrII C'CTAG_G
Cuts at: 0 310 1299
Size: 310 989
BamHI G'GATC_C
Cuts at: 0 495 1173 1299
Size: 495 678 126
Fragments arranged by size:
678 495 126
BanI G'GyrC_C
Cuts at: 0 386 579 1027 1299
Size: 386 193 448 272
Fragments arranged by size:
448 386 272 193
BanII G_rGCy'C
Cuts at: 0 222 1071 1241 1257 1299
Size: 222 849 170 16 42
Fragments arranged by size:
849 222 170 42 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1199 1299
Size: 1199 100
BbvCI CC'TCA_GC
Cuts at: 0 238 1299
Size: 238 1061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 1299
Size: 395 34 870
Fragments arranged by size:
870 395 34
BglI GCCn_nnn'nGGC
Cuts at: 0 577 1299
Size: 577 722
Bme1580I G_kGCm'C
Cuts at: 0 1241 1257 1299
Size: 1241 16 42
Fragments arranged by size:
1241 42 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 822 1299
Size: 822 477
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 875 1299
Size: 875 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 307 762 976 1072 1299
Size: 292 15 455 214 96 227
Fragments arranged by size:
455 292 227 214 96 15
Bpu10I CC'TnA_GC
Cuts at: 0 238 728 928 1299
Size: 238 490 200 371
Fragments arranged by size:
490 371 238 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 388 433 820 1299
Size: 388 45 387 479
Fragments arranged by size:
479 388 387 45
BsaBI GATnn'nnATC
Cuts at: 0 96 1299
Size: 96 1203
BsaWI w'CCGG_w
Cuts at: 0 878 998 1299
Size: 878 120 301
Fragments arranged by size:
878 301 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 596 626 1094 1124 1299
Size: 596 30 468 30 175
Fragments arranged by size:
596 468 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 838 1060 1299
Size: 373 465 222 239
Fragments arranged by size:
465 373 239 222
BseYI C'CCAG_C
Cuts at: 0 1169 1257 1299
Size: 1169 88 42
Fragments arranged by size:
1169 88 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1299
Size: 352 947
BsiHKAI G_wGCw'C
Cuts at: 0 697 1299
Size: 697 602
BsmBI CGTCTCn'nnnn_
Cuts at: 0 259 1299
Size: 259 1040
Bsp1286I G_dGCh'C
Cuts at: 0 222 697 1071 1241 1257 1299
Size: 222 475 374 170 16 42
Fragments arranged by size:
475 374 222 170 42 16
BspEI T'CCGG_A
Cuts at: 0 878 1299
Size: 878 421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1039 1299
Size: 1039 260
BsrDI GCAATG_nn'
Cuts at: 0 914 967 1299
Size: 914 53 332
Fragments arranged by size:
914 332 53
BssSI C'ACGA_G
Cuts at: 0 196 280 1299
Size: 196 84 1019
Fragments arranged by size:
1019 196 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 659 1299
Size: 659 640
BstYI r'GATC_y
Cuts at: 0 248 266 495 1173 1299
Size: 248 18 229 678 126
Fragments arranged by size:
678 248 229 126 18
Bsu36I CC'TnA_GG
Cuts at: 0 506 1299
Size: 506 793
BtgI C'CryG_G
Cuts at: 0 120 1109 1299
Size: 120 989 190
Fragments arranged by size:
989 190 120
BtsI GCAGTG_nn'
Cuts at: 0 695 1299
Size: 695 604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 572 607 757 792 1299
Size: 572 35 150 35 507
Fragments arranged by size:
572 507 150 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 71 431 1299
Size: 71 360 868
Fragments arranged by size:
868 360 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 144 189 292 307 762 976 1072
Size: 71 73 45 103 15 455 214 96
Cuts at: 1072 1199 1299
Size: 127 100
Fragments arranged by size:
455 214 127 103 100 96 73 71
45 15
EcoO109I rG'GnC_Cy
Cuts at: 0 446 1056 1237 1253 1254 1299
Size: 446 610 181 16 1 45
Fragments arranged by size:
610 446 181 45 16 1
HaeII r_GCGC'y
Cuts at: 0 159 1299
Size: 159 1140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 499 531 1299
Size: 109 32 358 32 768
Fragments arranged by size:
768 358 109 32 32
KpnI G_GTAC'C
Cuts at: 0 1031 1299
Size: 1031 268
MslI CAynn'nnrTG
Cuts at: 0 566 796 1299
Size: 566 230 503
Fragments arranged by size:
566 503 230
MspA1I CmG'CkG
Cuts at: 0 187 483 1299
Size: 187 296 816
Fragments arranged by size:
816 296 187
NspI r_CATG'y
Cuts at: 0 545 1299
Size: 545 754
PasI CC'CwG_GG
Cuts at: 0 1233 1299
Size: 1233 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 107 1299
Size: 107 1192
PfoI T'CCnGG_A
Cuts at: 0 269 1299
Size: 269 1030
PsiI TTA'TAA
Cuts at: 0 676 1299
Size: 676 623
PspOMI G'GGCC_C
Cuts at: 0 1237 1253 1299
Size: 1237 16 46
Fragments arranged by size:
1237 46 16
PstI C_TGCA'G
Cuts at: 0 408 488 702 1299
Size: 408 80 214 597
Fragments arranged by size:
597 408 214 80
PvuII CAG'CTG
Cuts at: 0 187 483 1299
Size: 187 296 816
Fragments arranged by size:
816 296 187
SexAI A'CCwGG_T
Cuts at: 0 1023 1299
Size: 1023 276
SfcI C'TryA_G
Cuts at: 0 404 484 698 969 1299
Size: 404 80 214 271 330
Fragments arranged by size:
404 330 271 214 80
StuI AGG'CCT
Cuts at: 0 145 202 1299
Size: 145 57 1097
Fragments arranged by size:
1097 145 57
StyI C'CwwG_G
Cuts at: 0 310 371 1299
Size: 310 61 928
Fragments arranged by size:
928 310 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 318 1034 1299
Size: 318 716 265
Fragments arranged by size:
716 318 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 1299
Size: 1094 32 173
Fragments arranged by size:
1094 173 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 497 837 1276 1299
Size: 349 148 340 439 23
Fragments arranged by size:
439 349 340 148 23
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BssSI BstXI BstYI Bsu36I BtgI BtsI
CspCI EarI Eco57MI EcoO109I HaeII Hin4I KpnI MslI
MspA1I NspI PasI PflMI PfoI PsiI PspOMI PstI
PvuII SexAI SfcI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI
BsaAI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI