Restriction map for
pFN21AE5563
(Linear) (Six-base) MAPSORT of: KIEE5563.seq Check: 8646 from: 1 to: 1236
KIEE5563.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AatII G_ACGT'C
Cuts at: 0 422 1236
Size: 422 814
AflII C'TTAA_G
Cuts at: 0 39 1236
Size: 39 1197
AflIII A'CryG_T
Cuts at: 0 891 1236
Size: 891 345
AleI CACnn'nnGTG
Cuts at: 0 1028 1236
Size: 1028 208
AlwNI CAG_nnn'CTG
Cuts at: 0 1228 1236
Size: 1228 8
ApaI G_GGCC'C
Cuts at: 0 667 1236
Size: 667 569
ApaLI G'TGCA_C
Cuts at: 0 1183 1236
Size: 1183 53
ApoI r'AATT_y
Cuts at: 0 392 451 1177 1236
Size: 392 59 726 59
Fragments arranged by size:
726 392 59 59
AseI AT'TA_AT
Cuts at: 0 605 1236
Size: 605 631
BamHI G'GATC_C
Cuts at: 0 480 1236
Size: 480 756
BanII G_rGCy'C
Cuts at: 0 667 1236
Size: 667 569
BbsI GAAGACnn'nnnn_
Cuts at: 0 719 989 1236
Size: 719 270 247
Fragments arranged by size:
719 270 247
BbvCI CC'TCA_GC
Cuts at: 0 412 550 1236
Size: 412 138 686
Fragments arranged by size:
686 412 138
BclI T'GATC_A
Cuts at: 0 671 734 924 1236
Size: 671 63 190 312
Fragments arranged by size:
671 312 190 63
Bme1580I G_kGCm'C
Cuts at: 0 667 948 1187 1236
Size: 667 281 239 49
Fragments arranged by size:
667 281 239 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1236
Size: 920 316
Bpu10I CC'TnA_GC
Cuts at: 0 140 412 550 1236
Size: 140 272 138 686
Fragments arranged by size:
686 272 140 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1236
Size: 164 1072
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 1236
Size: 410 826
BsaHI Gr'CG_yC
Cuts at: 0 419 1236
Size: 419 817
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 1236
Size: 425 30 781
Fragments arranged by size:
781 425 30
BseYI C'CCAG_C
Cuts at: 0 947 1236
Size: 947 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1236
Size: 1052 184
BsiHKAI G_wGCw'C
Cuts at: 0 195 598 1187 1236
Size: 195 403 589 49
Fragments arranged by size:
589 403 195 49
Bsp1286I G_dGCh'C
Cuts at: 0 195 598 667 948 1187 1236
Size: 195 403 69 281 239 49
Fragments arranged by size:
403 281 239 195 69 49
BsrDI GCAATG_nn'
Cuts at: 0 371 650 1236
Size: 371 279 586
Fragments arranged by size:
586 371 279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1124 1236
Size: 1124 112
BstBI TT'CG_AA
Cuts at: 0 855 1236
Size: 855 381
BstYI r'GATC_y
Cuts at: 0 68 480 1236
Size: 68 412 756
Fragments arranged by size:
756 412 68
BtgI C'CryG_G
Cuts at: 0 349 680 1236
Size: 349 331 556
Fragments arranged by size:
556 349 331
BtsI GCAGTG_nn'
Cuts at: 0 1023 1035 1236
Size: 1023 12 201
Fragments arranged by size:
1023 201 12
EaeI y'GGCC_r
Cuts at: 0 778 1236
Size: 778 458
EarI CTCTTCn'nnn_
Cuts at: 0 267 1236
Size: 267 969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1236
Size: 920 316
EcoO109I rG'GnC_Cy
Cuts at: 0 147 663 664 1009 1236
Size: 147 516 1 345 227
Fragments arranged by size:
516 345 227 147 1
EcoRI G'AATT_C
Cuts at: 0 392 451 1236
Size: 392 59 785
Fragments arranged by size:
785 392 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1006 1236
Size: 1006 230
HincII GTy'rAC
Cuts at: 0 1081 1236
Size: 1081 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 305 1236
Size: 159 146 931
Fragments arranged by size:
931 159 146
MslI CAynn'nnrTG
Cuts at: 0 1028 1236
Size: 1028 208
MspA1I CmG'CkG
Cuts at: 0 411 1086 1236
Size: 411 675 150
Fragments arranged by size:
675 411 150
NspI r_CATG'y
Cuts at: 0 895 1236
Size: 895 341
PasI CC'CwG_GG
Cuts at: 0 999 1236
Size: 999 237
PciI A'CATG_T
Cuts at: 0 891 1236
Size: 891 345
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 1236
Size: 129 1107
PspOMI G'GGCC_C
Cuts at: 0 663 1236
Size: 663 573
PstI C_TGCA'G
Cuts at: 0 380 1030 1236
Size: 380 650 206
Fragments arranged by size:
650 380 206
PvuII CAG'CTG
Cuts at: 0 411 1086 1236
Size: 411 675 150
Fragments arranged by size:
675 411 150
SfcI C'TryA_G
Cuts at: 0 376 627 958 1026 1236
Size: 376 251 331 68 210
Fragments arranged by size:
376 331 251 210 68
SmlI C'TyrA_G
Cuts at: 0 39 179 1236
Size: 39 140 1057
Fragments arranged by size:
1057 140 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 901 1236
Size: 901 335
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1236
Size: 425 32 779
Fragments arranged by size:
779 425 32
XmnI GAAnn'nnTTC
Cuts at: 0 26 646 1236
Size: 26 620 590
Fragments arranged by size:
620 590 26
ZraI GAC'GTC
Cuts at: 0 420 1236
Size: 420 816
Enzymes that do cut:
AatII AflII AflIII AleI AlwNI ApaI ApaLI ApoI
AseI BamHI BanII BbsI BbvCI BclI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BsrDI BstAPI BstBI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoO109I EcoRI FalI HincII MmeI MslI
MspA1I NspI PasI PciI PflMI PspOMI PstI PvuII
SfcI SmlI TaqII TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AgeI AhdI
AlfI AloI AscI AsiSI AvaI AvrII BaeI BanI
BbeI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI