Restriction map for
pFN21AE5562
(Linear) (Six-base) MAPSORT of: KIEE5562.seq Check: 7176 from: 1 to: 1236
KIEE5562.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
Acc65I G'GTAC_C
Cuts at: 0 597 1236
Size: 597 639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1236
Size: 247 989
AfeI AGC'GCT
Cuts at: 0 91 1236
Size: 91 1145
AflIII A'CryG_T
Cuts at: 0 116 1236
Size: 116 1120
AleI CACnn'nnGTG
Cuts at: 0 115 1236
Size: 115 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1236
Size: 150 1086
ApaLI G'TGCA_C
Cuts at: 0 913 1236
Size: 913 323
ApoI r'AATT_y
Cuts at: 0 126 1236
Size: 126 1110
AvaI C'yCGr_G
Cuts at: 0 772 1009 1236
Size: 772 237 227
Fragments arranged by size:
772 237 227
BanI G'GyrC_C
Cuts at: 0 597 852 1236
Size: 597 255 384
Fragments arranged by size:
597 384 255
BanII G_rGCy'C
Cuts at: 0 377 1236
Size: 377 859
BbeI G_GCGC'C
Cuts at: 0 856 1236
Size: 856 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 1152 1236
Size: 543 609 84
Fragments arranged by size:
609 543 84
BclI T'GATC_A
Cuts at: 0 632 1236
Size: 632 604
BglI GCCn_nnn'nGGC
Cuts at: 0 908 1236
Size: 908 328
Bme1580I G_kGCm'C
Cuts at: 0 917 1236
Size: 917 319
BmrI ACTGGGnnnn_n'
Cuts at: 0 606 1236
Size: 606 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1060 1236
Size: 140 920 176
Fragments arranged by size:
920 176 140
BsaHI Gr'CG_yC
Cuts at: 0 98 853 1236
Size: 98 755 383
Fragments arranged by size:
755 383 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1204 1234 1236
Size: 1204 30 2
Fragments arranged by size:
1204 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 357 1057 1236
Size: 357 700 179
Fragments arranged by size:
700 357 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 976 1158 1236
Size: 232 744 182 78
Fragments arranged by size:
744 232 182 78
BsiHKAI G_wGCw'C
Cuts at: 0 458 917 986 1236
Size: 458 459 69 250
Fragments arranged by size:
459 458 250 69
Bsp1286I G_dGCh'C
Cuts at: 0 377 458 917 986 1236
Size: 377 81 459 69 250
Fragments arranged by size:
459 377 250 81 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 446 887 1236
Size: 158 288 441 349
Fragments arranged by size:
441 349 288 158
BsrDI GCAATG_nn'
Cuts at: 0 138 1055 1236
Size: 138 917 181
Fragments arranged by size:
917 181 138
BsrGI T'GTAC_A
Cuts at: 0 884 1236
Size: 884 352
BssSI C'ACGA_G
Cuts at: 0 562 1236
Size: 562 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 962 990 1236
Size: 962 28 246
Fragments arranged by size:
962 246 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1038 1209 1236
Size: 1038 171 27
Fragments arranged by size:
1038 171 27
BstYI r'GATC_y
Cuts at: 0 264 1034 1236
Size: 264 770 202
Fragments arranged by size:
770 264 202
BtgI C'CryG_G
Cuts at: 0 848 897 1236
Size: 848 49 339
Fragments arranged by size:
848 339 49
BtsI GCAGTG_nn'
Cuts at: 0 216 1236
Size: 216 1020
ClaI AT'CG_AT
Cuts at: 0 185 1236
Size: 185 1051
EaeI y'GGCC_r
Cuts at: 0 827 951 1028 1236
Size: 827 124 77 208
Fragments arranged by size:
827 208 124 77
EarI CTCTTCn'nnn_
Cuts at: 0 760 1236
Size: 760 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 453 1236
Size: 453 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 247 1060 1236
Size: 140 107 813 176
Fragments arranged by size:
813 176 140 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1042 1236
Size: 1042 194
EcoO109I rG'GnC_Cy
Cuts at: 0 236 318 1017 1236
Size: 236 82 699 219
Fragments arranged by size:
699 236 219 82
EcoRI G'AATT_C
Cuts at: 0 126 1236
Size: 126 1110
EcoRV GAT'ATC
Cuts at: 0 288 1236
Size: 288 948
HaeII r_GCGC'y
Cuts at: 0 93 819 848 856 1236
Size: 93 726 29 8 380
Fragments arranged by size:
726 380 93 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1204 1214 1236
Size: 1182 22 10 22
Fragments arranged by size:
1182 22 22 10
KasI G'GCGC_C
Cuts at: 0 852 1236
Size: 852 384
KpnI G_GTAC'C
Cuts at: 0 601 1236
Size: 601 635
MfeI C'AATT_G
Cuts at: 0 37 1236
Size: 37 1199
MscI TGG'CCA
Cuts at: 0 829 1030 1236
Size: 829 201 206
Fragments arranged by size:
829 206 201
MslI CAynn'nnrTG
Cuts at: 0 115 1189 1236
Size: 115 1074 47
Fragments arranged by size:
1074 115 47
MspA1I CmG'CkG
Cuts at: 0 813 899 1073 1236
Size: 813 86 174 163
Fragments arranged by size:
813 174 163 86
NarI GG'CG_CC
Cuts at: 0 853 1236
Size: 853 383
PasI CC'CwG_GG
Cuts at: 0 519 1236
Size: 519 717
PfoI T'CCnGG_A
Cuts at: 0 1037 1236
Size: 1037 199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1236
Size: 1096 32 108
Fragments arranged by size:
1096 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 236 318 1017 1236
Size: 236 82 699 219
Fragments arranged by size:
699 236 219 82
PshAI GACnn'nnGTC
Cuts at: 0 724 1236
Size: 724 512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 868 900 1236
Size: 868 32 336
Fragments arranged by size:
868 336 32
PstI C_TGCA'G
Cuts at: 0 272 498 677 823 1102 1236
Size: 272 226 179 146 279 134
Fragments arranged by size:
279 272 226 179 146 134
SacII CC_GC'GG
Cuts at: 0 900 1236
Size: 900 336
SanDI GG'GwC_CC
Cuts at: 0 1017 1236
Size: 1017 219
ScaI AGT'ACT
Cuts at: 0 616 1236
Size: 616 620
SfcI C'TryA_G
Cuts at: 0 268 494 673 819 1098 1236
Size: 268 226 179 146 279 138
Fragments arranged by size:
279 268 226 179 146 138
SfoI GGC'GCC
Cuts at: 0 854 1236
Size: 854 382
SmaI CCC'GGG
Cuts at: 0 774 1236
Size: 774 462
SspI AAT'ATT
Cuts at: 0 10 1236
Size: 10 1226
StyI C'CwwG_G
Cuts at: 0 260 1236
Size: 260 976
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1146 1236
Size: 1146 90
TatI w'GTAC_w
Cuts at: 0 614 884 1236
Size: 614 270 352
Fragments arranged by size:
614 352 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1236
Size: 1123 32 81
Fragments arranged by size:
1123 81 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1236
Size: 636 600
XmaI C'CCGG_G
Cuts at: 0 772 1236
Size: 772 464
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BclI BglI Bme1580I
BmrI BpmI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtsI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I KasI KpnI MfeI MscI
MslI MspA1I NarI PasI PfoI PpiI PpuMI PshAI
PsrI PstI SacII SanDI ScaI SfcI SfoI SmaI
SspI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI
DraIII DrdI EagI EcoICRI FalI FseI FspI FspAI
HincII HindIII HpaI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PsiI PspOMI PspXI PvuI PvuII
RsrII SacI SalI SapI SbfI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XbaI XhoI XmnI ZraI