Restriction map for
pFN21AE5561
(Linear) (Six-base) MAPSORT of: KIEE5561.seq Check: 9254 from: 1 to: 1266
KIEE5561.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AclI AA'CG_TT
Cuts at: 0 708 1266
Size: 708 558
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 375 1147 1266
Size: 79 296 772 119
Fragments arranged by size:
772 296 119 79
AflII C'TTAA_G
Cuts at: 0 226 1266
Size: 226 1040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 1266
Size: 550 716
ApoI r'AATT_y
Cuts at: 0 52 800 1266
Size: 52 748 466
Fragments arranged by size:
748 466 52
BanII G_rGCy'C
Cuts at: 0 452 1070 1266
Size: 452 618 196
Fragments arranged by size:
618 452 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 765 794 1103 1153 1266
Size: 765 29 309 50 113
Fragments arranged by size:
765 309 113 50 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 534 1266
Size: 534 732
BlpI GC'TnA_GC
Cuts at: 0 368 1266
Size: 368 898
Bme1580I G_kGCm'C
Cuts at: 0 23 1266
Size: 23 1243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 371 463 1266
Size: 31 340 92 803
Fragments arranged by size:
803 340 92 31
Bpu10I CC'TnA_GC
Cuts at: 0 77 1266
Size: 77 1189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 420 450 485 515 534 564 1266
Size: 420 30 35 30 19 30 702
Fragments arranged by size:
702 420 35 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 140 1266
Size: 140 1126
BseYI C'CCAG_C
Cuts at: 0 60 1266
Size: 60 1206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1266
Size: 1087 179
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1266
Size: 1070 196
BsmI GAATG_Cn'
Cuts at: 0 71 1266
Size: 71 1195
Bsp1286I G_dGCh'C
Cuts at: 0 23 452 1070 1266
Size: 23 429 618 196
Fragments arranged by size:
618 429 196 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 622 636 649 1266
Size: 622 14 13 617
Fragments arranged by size:
622 617 14 13
BsrDI GCAATG_nn'
Cuts at: 0 52 588 1199 1266
Size: 52 536 611 67
Fragments arranged by size:
611 536 67 52
BsrGI T'GTAC_A
Cuts at: 0 356 512 1266
Size: 356 156 754
Fragments arranged by size:
754 356 156
BstEII G'GTnAC_C
Cuts at: 0 1209 1266
Size: 1209 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 334 1266
Size: 334 932
BstYI r'GATC_y
Cuts at: 0 983 1266
Size: 983 283
BtgI C'CryG_G
Cuts at: 0 462 489 1266
Size: 462 27 777
Fragments arranged by size:
777 462 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 952 1266
Size: 952 314
DraIII CAC_nnn'GTG
Cuts at: 0 909 1083 1266
Size: 909 174 183
Fragments arranged by size:
909 183 174
EarI CTCTTCn'nnn_
Cuts at: 0 382 1266
Size: 382 884
EcoICRI GAG'CTC
Cuts at: 0 1068 1266
Size: 1068 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 79 371 375 463 1147 1266
Size: 31 48 292 4 88 684 119
Fragments arranged by size:
684 292 119 88 48 31 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 1266
Size: 181 1085
EcoRI G'AATT_C
Cuts at: 0 52 1266
Size: 52 1214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 760 1266
Size: 760 506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 866 898 1266
Size: 420 32 414 32 368
Fragments arranged by size:
420 414 368 32 32
MslI CAynn'nnrTG
Cuts at: 0 837 971 1266
Size: 837 134 295
Fragments arranged by size:
837 295 134
MspA1I CmG'CkG
Cuts at: 0 367 1255 1266
Size: 367 888 11
Fragments arranged by size:
888 367 11
NcoI C'CATG_G
Cuts at: 0 462 489 1266
Size: 462 27 777
Fragments arranged by size:
777 462 27
NspI r_CATG'y
Cuts at: 0 753 1034 1266
Size: 753 281 232
Fragments arranged by size:
753 281 232
PasI CC'CwG_GG
Cuts at: 0 940 1266
Size: 940 326
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 1083 1266
Size: 468 615 183
Fragments arranged by size:
615 468 183
PfoI T'CCnGG_A
Cuts at: 0 348 1061 1266
Size: 348 713 205
Fragments arranged by size:
713 348 205
PsiI TTA'TAA
Cuts at: 0 950 1266
Size: 950 316
PstI C_TGCA'G
Cuts at: 0 539 633 660 1266
Size: 539 94 27 606
Fragments arranged by size:
606 539 94 27
SacI G_AGCT'C
Cuts at: 0 1070 1266
Size: 1070 196
SapI GCTCTTCn'nnn_
Cuts at: 0 382 1266
Size: 382 884
SbfI CC_TGCA'GG
Cuts at: 0 539 633 1266
Size: 539 94 633
Fragments arranged by size:
633 539 94
SfcI C'TryA_G
Cuts at: 0 135 519 535 629 656 1041 1266
Size: 135 384 16 94 27 385 225
Fragments arranged by size:
385 384 225 135 94 27 16
SmlI C'TyrA_G
Cuts at: 0 226 1266
Size: 226 1040
SphI G_CATG'C
Cuts at: 0 753 1266
Size: 753 513
SspI AAT'ATT
Cuts at: 0 271 1266
Size: 271 995
StuI AGG'CCT
Cuts at: 0 1233 1266
Size: 1233 33
StyI C'CwwG_G
Cuts at: 0 462 489 545 1266
Size: 462 27 56 721
Fragments arranged by size:
721 462 56 27
TatI w'GTAC_w
Cuts at: 0 356 512 1266
Size: 356 156 754
Fragments arranged by size:
754 356 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1266
Size: 1096 32 138
Fragments arranged by size:
1096 138 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 487 1266
Size: 487 779
Enzymes that do cut:
AclI AcuI AflII AlfI ApoI BanII BbsI BciVI
BlpI Bme1580I BpmI Bpu10I BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstEII BstXI
BstYI BtgI BtgZI DraIII EarI EcoICRI Eco57MI EcoNI
EcoRI FalI Hin4I MslI MspA1I NcoI NspI PasI
PflMI PfoI PsiI PstI SacI SapI SbfI SfcI
SmlI SphI SspI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EaeI EagI EciI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI