Restriction map for
pFN21AE5559
(Linear) (Six-base) MAPSORT of: KIEE5559.seq Check: 5036 from: 1 to: 1212
KIEE5559.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
Acc65I G'GTAC_C
Cuts at: 0 286 851 1212
Size: 286 565 361
Fragments arranged by size:
565 361 286
AlwNI CAG_nnn'CTG
Cuts at: 0 663 1140 1212
Size: 663 477 72
Fragments arranged by size:
663 477 72
ApaI G_GGCC'C
Cuts at: 0 82 201 1212
Size: 82 119 1011
Fragments arranged by size:
1011 119 82
AvaI C'yCGr_G
Cuts at: 0 82 158 1212
Size: 82 76 1054
Fragments arranged by size:
1054 82 76
AvrII C'CTAG_G
Cuts at: 0 246 282 417 1212
Size: 246 36 135 795
Fragments arranged by size:
795 246 135 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 278 311 1212
Size: 278 33 901
Fragments arranged by size:
901 278 33
BamHI G'GATC_C
Cuts at: 0 547 1212
Size: 547 665
BanI G'GyrC_C
Cuts at: 0 286 851 1212
Size: 286 565 361
Fragments arranged by size:
565 361 286
BanII G_rGCy'C
Cuts at: 0 82 201 818 1184 1212
Size: 82 119 617 366 28
Fragments arranged by size:
617 366 119 82 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1212
Size: 253 959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 16 50 1212
Size: 16 34 1162
Fragments arranged by size:
1162 34 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 279 565 1212
Size: 279 286 647
Fragments arranged by size:
647 286 279
BglI GCCn_nnn'nGGC
Cuts at: 0 86 1212
Size: 86 1126
BglII A'GATC_T
Cuts at: 0 311 1212
Size: 311 901
BlpI GC'TnA_GC
Cuts at: 0 1141 1212
Size: 1141 71
Bme1580I G_kGCm'C
Cuts at: 0 82 119 201 1212
Size: 82 37 82 1011
Fragments arranged by size:
1011 82 82 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 684 1212
Size: 684 528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 1212
Size: 735 477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 734 1212
Size: 547 187 478
Fragments arranged by size:
547 478 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 925 1212
Size: 415 510 287
Fragments arranged by size:
510 415 287
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1212
Size: 874 338
BsaAI yAC'GTr
Cuts at: 0 576 1212
Size: 576 636
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 462 637 1212
Size: 462 175 575
Fragments arranged by size:
575 462 175
BseYI C'CCAG_C
Cuts at: 0 183 193 567 1017 1034 1086 1212
Size: 183 10 374 450 17 52 126
Fragments arranged by size:
450 374 183 126 52 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 973 1008 1212
Size: 149 824 35 204
Fragments arranged by size:
824 204 149 35
BsiEI CG_ry'CG
Cuts at: 0 76 107 219 1212
Size: 76 31 112 993
Fragments arranged by size:
993 112 76 31
BsiHKAI G_wGCw'C
Cuts at: 0 214 773 818 1212
Size: 214 559 45 394
Fragments arranged by size:
559 394 214 45
BsmI GAATG_Cn'
Cuts at: 0 1200 1212
Size: 1200 12
Bsp1286I G_dGCh'C
Cuts at: 0 82 119 201 214 773 818 1184 1212
Size: 82 37 82 13 559 45 366 28
Fragments arranged by size:
559 366 82 82 45 37 28 13
BsrFI r'CCGG_y
Cuts at: 0 102 106 214 1212
Size: 102 4 108 998
Fragments arranged by size:
998 108 102 4
BssHII G'CGCG_C
Cuts at: 0 1127 1212
Size: 1127 85
BstXI CCAn_nnnn'nTGG
Cuts at: 0 829 990 1212
Size: 829 161 222
Fragments arranged by size:
829 222 161
BstYI r'GATC_y
Cuts at: 0 311 547 1212
Size: 311 236 665
Fragments arranged by size:
665 311 236
BtgI C'CryG_G
Cuts at: 0 722 1028 1212
Size: 722 306 184
Fragments arranged by size:
722 306 184
BtsI GCAGTG_nn'
Cuts at: 0 54 1068 1212
Size: 54 1014 144
Fragments arranged by size:
1014 144 54
EaeI y'GGCC_r
Cuts at: 0 73 104 216 725 1212
Size: 73 31 112 509 487
Fragments arranged by size:
509 487 112 73 31
EagI C'GGCC_G
Cuts at: 0 73 104 216 1212
Size: 73 31 112 996
Fragments arranged by size:
996 112 73 31
EcoICRI GAG'CTC
Cuts at: 0 816 1212
Size: 816 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 734 1212
Size: 547 187 478
Fragments arranged by size:
547 478 187
EcoO109I rG'GnC_Cy
Cuts at: 0 79 197 198 660 914 1212
Size: 79 118 1 462 254 298
Fragments arranged by size:
462 298 254 118 79 1
FseI GG_CCGG'CC
Cuts at: 0 106 1212
Size: 106 1106
HaeII r_GCGC'y
Cuts at: 0 190 1212
Size: 190 1022
KpnI G_GTAC'C
Cuts at: 0 290 855 1212
Size: 290 565 357
Fragments arranged by size:
565 357 290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 816 1212
Size: 424 392 396
Fragments arranged by size:
424 396 392
MscI TGG'CCA
Cuts at: 0 727 1212
Size: 727 485
MslI CAynn'nnrTG
Cuts at: 0 229 687 1212
Size: 229 458 525
Fragments arranged by size:
525 458 229
MspA1I CmG'CkG
Cuts at: 0 193 567 807 1017 1149 1212
Size: 193 374 240 210 132 63
Fragments arranged by size:
374 240 210 193 132 63
NaeI GCC'GGC
Cuts at: 0 104 108 1212
Size: 104 4 1104
Fragments arranged by size:
1104 104 4
NcoI C'CATG_G
Cuts at: 0 722 1212
Size: 722 490
NgoMIV G'CCGG_C
Cuts at: 0 102 106 1212
Size: 102 4 1106
Fragments arranged by size:
1106 102 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1212
Size: 663 549
PshAI GACnn'nnGTC
Cuts at: 0 646 1212
Size: 646 566
PspOMI G'GGCC_C
Cuts at: 0 78 197 1212
Size: 78 119 1015
Fragments arranged by size:
1015 119 78
PstI C_TGCA'G
Cuts at: 0 702 1212
Size: 702 510
PvuII CAG'CTG
Cuts at: 0 567 807 1017 1149 1212
Size: 567 240 210 132 63
Fragments arranged by size:
567 240 210 132 63
SacI G_AGCT'C
Cuts at: 0 818 1212
Size: 818 394
SfcI C'TryA_G
Cuts at: 0 344 698 1132 1212
Size: 344 354 434 80
Fragments arranged by size:
434 354 344 80
SmaI CCC'GGG
Cuts at: 0 84 1212
Size: 84 1128
SmlI C'TyrA_G
Cuts at: 0 430 940 1212
Size: 430 510 272
Fragments arranged by size:
510 430 272
StuI AGG'CCT
Cuts at: 0 715 1212
Size: 715 497
StyI C'CwwG_G
Cuts at: 0 152 246 282 417 722 1212
Size: 152 94 36 135 305 490
Fragments arranged by size:
490 305 152 135 94 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 1212
Size: 46 32 1134
Fragments arranged by size:
1134 46 32
Tth111I GACn'n_nGTC
Cuts at: 0 583 1212
Size: 583 629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 1212
Size: 615 597
XmaI C'CCGG_G
Cuts at: 0 82 1212
Size: 82 1130
Enzymes that do cut:
Acc65I AlwNI ApaI AvaI AvrII BaeI BamHI BanI
BanII BbsI BcgI BciVI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrFI BssHII BstXI
BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoO109I
FseI HaeII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PflMI PshAI PspOMI PstI PvuII SacI
SfcI SmaI SmlI StuI StyI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI BbeI BbvCI BclI BfrBI BmgBI BmtI
Bpu10I BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoNI EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI XbaI
XhoI XmnI ZraI