(Linear) (Six-base) MAPSORT of: KIEE5558.seq  Check: 7619  from: 1  to: 1143



KIEE5558.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391    1109    1143 
   Size:        391     718      34

  Fragments arranged by size: 

                 718     391      34
 
AflII C'TTAA_G

Cuts at:      0    1102    1143 
   Size:       1102      41
 
ApoI r'AATT_y

Cuts at:      0      49     401    1143 
   Size:         49     352     742

  Fragments arranged by size: 

                 742     352      49
 
BanII G_rGCy'C

Cuts at:      0    1080    1143 
   Size:       1080      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     322     486    1143 
   Size:        322     164     657

  Fragments arranged by size: 

                 657     322     164
 
BglII A'GATC_T

Cuts at:      0     351    1143 
   Size:        351     792
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1081    1143 
   Size:       1081      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1143 
   Size:         37    1106
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1143 
   Size:          4    1139
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181    1143 
   Size:        181     962
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    1143 
   Size:        256     887
 
BsiHKAI G_wGCw'C

Cuts at:      0    1080    1143 
   Size:       1080      63
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11    1143 
   Size:         11    1132
 
Bsp1286I G_dGCh'C

Cuts at:      0    1080    1143 
   Size:       1080      63
 
BsrBI CCG'CTC

Cuts at:      0      21    1143 
   Size:         21    1122
 
BstYI r'GATC_y

Cuts at:      0     351     505    1143 
   Size:        351     154     638

  Fragments arranged by size: 

                 638     351     154
 
Bsu36I CC'TnA_GG

Cuts at:      0     971     995    1143 
   Size:        971      24     148

  Fragments arranged by size: 

                 971     148      24
 
ClaI AT'CG_AT

Cuts at:      0     833    1143 
   Size:        833     310
 
EaeI y'GGCC_r

Cuts at:      0      22     226    1028    1143 
   Size:         22     204     802     115

  Fragments arranged by size: 

                 802     204     115      22
 
EcoICRI GAG'CTC

Cuts at:      0    1078    1143 
   Size:       1078      65
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     391    1109    1143 
   Size:         37     354     718      34

  Fragments arranged by size: 

                 718     354      37      34
 
EcoRI G'AATT_C

Cuts at:      0     401    1143 
   Size:        401     742
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     634    1143 
   Size:        634     509
 
HaeII r_GCGC'y

Cuts at:      0      38    1143 
   Size:         38    1105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     309     341    1143 
   Size:        309      32     802

  Fragments arranged by size: 

                 802     309      32
 
HindIII A'AGCT_T

Cuts at:      0     660    1001    1143 
   Size:        660     341     142

  Fragments arranged by size: 

                 660     341     142
 
MfeI C'AATT_G

Cuts at:      0     983    1143 
   Size:        983     160
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      97    1143 
   Size:         97    1046
 
MscI TGG'CCA

Cuts at:      0     228    1030    1143 
   Size:        228     802     113

  Fragments arranged by size: 

                 802     228     113
 
MslI CAynn'nnrTG

Cuts at:      0     263     746    1143 
   Size:        263     483     397

  Fragments arranged by size: 

                 483     397     263
 
MspA1I CmG'CkG

Cuts at:      0    1087    1143 
   Size:       1087      56
 
NspI r_CATG'y

Cuts at:      0     425    1143 
   Size:        425     718
 
PsiI TTA'TAA

Cuts at:      0     650    1143 
   Size:        650     493
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     380     412    1143 
   Size:        380      32     731

  Fragments arranged by size: 

                 731     380      32
 
PvuII CAG'CTG

Cuts at:      0    1087    1143 
   Size:       1087      56
 
SacI G_AGCT'C

Cuts at:      0    1080    1143 
   Size:       1080      63
 
SmlI C'TyrA_G

Cuts at:      0    1102    1143 
   Size:       1102      41
 
SspI AAT'ATT

Cuts at:      0     636    1143 
   Size:        636     507
 
TatI w'GTAC_w

Cuts at:      0     304    1143 
   Size:        304     839
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     159    1143 
   Size:        159     984
 

 Enzymes that do cut:

     AcuI    AflII     ApoI    BanII     BbsI    BglII     BmrI     BpmI
    BsaHI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BstYI
   Bsu36I     ClaI     EaeI  EcoICRI  Eco57MI    EcoRI     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NspI
     PsiI     PsrI    PvuII     SacI     SmlI     SspI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI