Restriction map for
pFN21AE5557
(Linear) (Six-base) MAPSORT of: KIEE5557.seq Check: 7444 from: 1 to: 1131
KIEE5557.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 971 1131
Size: 971 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1131
Size: 934 197
AlwNI CAG_nnn'CTG
Cuts at: 0 897 1131
Size: 897 234
ApaI G_GGCC'C
Cuts at: 0 31 1131
Size: 31 1100
ApoI r'AATT_y
Cuts at: 0 293 1131
Size: 293 838
AvaI C'yCGr_G
Cuts at: 0 287 663 1131
Size: 287 376 468
Fragments arranged by size:
468 376 287
BamHI G'GATC_C
Cuts at: 0 35 1131
Size: 35 1096
BanI G'GyrC_C
Cuts at: 0 6 907 1131
Size: 6 901 224
Fragments arranged by size:
901 224 6
BanII G_rGCy'C
Cuts at: 0 31 156 434 782 1131
Size: 31 125 278 348 349
Fragments arranged by size:
349 348 278 125 31
BbeI G_GCGC'C
Cuts at: 0 10 1131
Size: 10 1121
BbvCI CC'TCA_GC
Cuts at: 0 585 1131
Size: 585 546
BclI T'GATC_A
Cuts at: 0 855 1131
Size: 855 276
BglI GCCn_nnn'nGGC
Cuts at: 0 115 1131
Size: 115 1016
BlpI GC'TnA_GC
Cuts at: 0 748 1131
Size: 748 383
Bme1580I G_kGCm'C
Cuts at: 0 31 910 1131
Size: 31 879 221
Fragments arranged by size:
879 221 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 564 1131
Size: 564 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 313 1131
Size: 313 818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1131
Size: 766 365
Bpu10I CC'TnA_GC
Cuts at: 0 275 585 1131
Size: 275 310 546
Fragments arranged by size:
546 310 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 1131
Size: 346 785
BsaHI Gr'CG_yC
Cuts at: 0 7 1131
Size: 7 1124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1131
Size: 175 30 926
Fragments arranged by size:
926 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 753 1131
Size: 753 378
BseYI C'CCAG_C
Cuts at: 0 921 1131
Size: 921 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1042 1131
Size: 949 93 89
Fragments arranged by size:
949 93 89
BsiHKAI G_wGCw'C
Cuts at: 0 749 782 1128 1131
Size: 749 33 346 3
Fragments arranged by size:
749 346 33 3
BsmI GAATG_Cn'
Cuts at: 0 720 1050 1131
Size: 720 330 81
Fragments arranged by size:
720 330 81
BsmBI CGTCTCn'nnnn_
Cuts at: 0 84 1131
Size: 84 1047
Bsp1286I G_dGCh'C
Cuts at: 0 31 156 434 749 782 910 1128 1131
Size: 31 125 278 315 33 128 218 3
Fragments arranged by size:
315 278 218 128 125 33 31 3
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 971 1131
Size: 971 160
BsrFI r'CCGG_y
Cuts at: 0 186 449 1131
Size: 186 263 682
Fragments arranged by size:
682 263 186
BssSI C'ACGA_G
Cuts at: 0 772 1131
Size: 772 359
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1056 1065 1131
Size: 1056 9 66
Fragments arranged by size:
1056 66 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1119 1131
Size: 1119 12
BstYI r'GATC_y
Cuts at: 0 35 1131
Size: 35 1096
EaeI y'GGCC_r
Cuts at: 0 48 116 417 1131
Size: 48 68 301 714
Fragments arranged by size:
714 301 68 48
EarI CTCTTCn'nnn_
Cuts at: 0 73 1131
Size: 73 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 756 1131
Size: 756 375
EcoICRI GAG'CTC
Cuts at: 0 780 1131
Size: 780 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 934 1131
Size: 766 168 197
Fragments arranged by size:
766 197 168
EcoO109I rG'GnC_Cy
Cuts at: 0 28 1131
Size: 28 1103
EcoRI G'AATT_C
Cuts at: 0 293 1131
Size: 293 838
FspI TGC'GCA
Cuts at: 0 719 1131
Size: 719 412
HaeII r_GCGC'y
Cuts at: 0 10 1131
Size: 10 1121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1131
Size: 175 32 924
Fragments arranged by size:
924 175 32
KasI G'GCGC_C
Cuts at: 0 6 1131
Size: 6 1125
MscI TGG'CCA
Cuts at: 0 419 1131
Size: 419 712
MslI CAynn'nnrTG
Cuts at: 0 565 1131
Size: 565 566
MspA1I CmG'CkG
Cuts at: 0 100 106 210 246 978 1131
Size: 100 6 104 36 732 153
Fragments arranged by size:
732 153 104 100 36 6
NarI GG'CG_CC
Cuts at: 0 7 1131
Size: 7 1124
NspI r_CATG'y
Cuts at: 0 574 1131
Size: 574 557
PasI CC'CwG_GG
Cuts at: 0 361 993 1131
Size: 361 632 138
Fragments arranged by size:
632 361 138
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 850 1131
Size: 453 397 281
Fragments arranged by size:
453 397 281
PfoI T'CCnGG_A
Cuts at: 0 1000 1131
Size: 1000 131
PspOMI G'GGCC_C
Cuts at: 0 27 1131
Size: 27 1104
PstI C_TGCA'G
Cuts at: 0 251 1131
Size: 251 880
PvuII CAG'CTG
Cuts at: 0 246 978 1131
Size: 246 732 153
Fragments arranged by size:
732 246 153
SacI G_AGCT'C
Cuts at: 0 782 1131
Size: 782 349
ScaI AGT'ACT
Cuts at: 0 553 865 1131
Size: 553 312 266
Fragments arranged by size:
553 312 266
SexAI A'CCwGG_T
Cuts at: 0 740 1131
Size: 740 391
SfcI C'TryA_G
Cuts at: 0 247 1131
Size: 247 884
SfoI GGC'GCC
Cuts at: 0 8 1131
Size: 8 1123
StyI C'CwwG_G
Cuts at: 0 51 849 1131
Size: 51 798 282
Fragments arranged by size:
798 282 51
TatI w'GTAC_w
Cuts at: 0 387 551 863 1131
Size: 387 164 312 268
Fragments arranged by size:
387 312 268 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 1131
Size: 497 32 602
Fragments arranged by size:
602 497 32
Tth111I GACn'n_nGTC
Cuts at: 0 657 1131
Size: 657 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 790 1131
Size: 474 316 341
Fragments arranged by size:
474 341 316
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbvCI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssSI
BstAPI BstXI BstYI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I KasI MscI MslI
MspA1I NarI NspI PasI PflMI PfoI PspOMI PstI
PvuII SacI ScaI SexAI SfcI SfoI StyI TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BciVI BfrBI BglII BmgBI
BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoNI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI
ZraI