(Linear) (Six-base) MAPSORT of: KIEE5556.seq  Check: 6757  from: 1  to: 1122



KIEE5556.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:05  ..

AccI GT'mk_AC

Cuts at:      0     962    1122 
   Size:        962     160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     886    1122 
   Size:        178     708     236

  Fragments arranged by size: 

                 708     236     178
 
ApoI r'AATT_y

Cuts at:      0     274     872    1122 
   Size:        274     598     250

  Fragments arranged by size: 

                 598     274     250
 
AvaI C'yCGr_G

Cuts at:      0     599    1122 
   Size:        599     523
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     213    1122 
   Size:        213     909
 
BclI T'GATC_A

Cuts at:      0     975    1122 
   Size:        975     147
 
BfrBI ATG'CAT

Cuts at:      0     649    1122 
   Size:        649     473
 
BlpI GC'TnA_GC

Cuts at:      0      47    1122 
   Size:         47    1075
 
BsaHI Gr'CG_yC

Cuts at:      0     941    1122 
   Size:        941     181
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     302    1122 
   Size:        302     820
 
BspHI T'CATG_A

Cuts at:      0     711    1122 
   Size:        711     411
 
BstYI r'GATC_y

Cuts at:      0     639    1122 
   Size:        639     483
 
BtgI C'CryG_G

Cuts at:      0     330    1122 
   Size:        330     792
 
BtsI GCAGTG_nn'

Cuts at:      0    1042    1122 
   Size:       1042      80
 
DraI TTT'AAA

Cuts at:      0     677    1122 
   Size:        677     445
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     523    1122 
   Size:         86     437     599

  Fragments arranged by size: 

                 599     437      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     886    1122 
   Size:        178     708     236

  Fragments arranged by size: 

                 708     236     178
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     127     721    1122 
   Size:        127     594     401

  Fragments arranged by size: 

                 594     401     127
 
HindIII A'AGCT_T

Cuts at:      0     239    1122 
   Size:        239     883
 
MslI CAynn'nnrTG

Cuts at:      0     730    1122 
   Size:        730     392
 
NdeI CA'TA_TG

Cuts at:      0     753    1122 
   Size:        753     369
 
NruI TCG'CGA

Cuts at:      0     281    1122 
   Size:        281     841
 
NsiI A_TGCA'T

Cuts at:      0     651    1122 
   Size:        651     471
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336    1122 
   Size:        336     786
 
PfoI T'CCnGG_A

Cuts at:      0     218    1122 
   Size:        218     904
 
PspXI vC'TCGA_Gb

Cuts at:      0     599    1122 
   Size:        599     523
 
PstI C_TGCA'G

Cuts at:      0     311    1122 
   Size:        311     811
 
SfcI C'TryA_G

Cuts at:      0     307    1122 
   Size:        307     815
 
SmlI C'TyrA_G

Cuts at:      0     599    1122 
   Size:        599     523
 
TatI w'GTAC_w

Cuts at:      0     413    1122 
   Size:        413     709
 
XhoI C'TCGA_G

Cuts at:      0     599    1122 
   Size:        599     523
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AvaI    BciVI     BclI    BfrBI     BlpI
    BsaHI    BsmBI    BspHI    BstYI     BtgI     BtsI     DraI     EarI
  Eco57MI     FalI  HindIII     MslI     NdeI     NruI     NsiI    PflMI
     PfoI    PspXI     PstI     SfcI     SmlI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI