Restriction map for
pFN21AE5555
(Linear) (Six-base) MAPSORT of: KIEE5555.seq Check: 9434 from: 1 to: 1101
KIEE5555.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
Acc65I G'GTAC_C
Cuts at: 0 865 1101
Size: 865 236
AclI AA'CG_TT
Cuts at: 0 11 1101
Size: 11 1090
AfeI AGC'GCT
Cuts at: 0 1011 1101
Size: 1011 90
AgeI A'CCGG_T
Cuts at: 0 480 1101
Size: 480 621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 1101
Size: 290 811
ApoI r'AATT_y
Cuts at: 0 103 440 1101
Size: 103 337 661
Fragments arranged by size:
661 337 103
AseI AT'TA_AT
Cuts at: 0 242 1101
Size: 242 859
AvaI C'yCGr_G
Cuts at: 0 492 572 1101
Size: 492 80 529
Fragments arranged by size:
529 492 80
BanI G'GyrC_C
Cuts at: 0 865 1101
Size: 865 236
BanII G_rGCy'C
Cuts at: 0 499 1101
Size: 499 602
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 637 1101
Size: 603 34 464
Fragments arranged by size:
603 464 34
BglI GCCn_nnn'nGGC
Cuts at: 0 775 1101
Size: 775 326
Bme1580I G_kGCm'C
Cuts at: 0 341 573 1101
Size: 341 232 528
Fragments arranged by size:
528 341 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 13 1101
Size: 13 1088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 556 1101
Size: 437 119 545
Fragments arranged by size:
545 437 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 574 1101
Size: 574 527
BsaWI w'CCGG_w
Cuts at: 0 480 1101
Size: 480 621
BseYI C'CCAG_C
Cuts at: 0 293 886 1101
Size: 293 593 215
Fragments arranged by size:
593 293 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1101
Size: 695 406
BsiEI CG_ry'CG
Cuts at: 0 788 1101
Size: 788 313
BsiHKAI G_wGCw'C
Cuts at: 0 822 1101
Size: 822 279
BsmI GAATG_Cn'
Cuts at: 0 103 1101
Size: 103 998
Bsp1286I G_dGCh'C
Cuts at: 0 341 499 573 822 1101
Size: 341 158 74 249 279
Fragments arranged by size:
341 279 249 158 74
BsrDI GCAATG_nn'
Cuts at: 0 290 867 1101
Size: 290 577 234
Fragments arranged by size:
577 290 234
BsrFI r'CCGG_y
Cuts at: 0 480 1101
Size: 480 621
BstAPI GCAn_nnn'nTGC
Cuts at: 0 289 1101
Size: 289 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 801 1101
Size: 801 300
BtgI C'CryG_G
Cuts at: 0 782 788 1101
Size: 782 6 313
Fragments arranged by size:
782 313 6
EaeI y'GGCC_r
Cuts at: 0 767 785 791 1101
Size: 767 18 6 310
Fragments arranged by size:
767 310 18 6
EagI C'GGCC_G
Cuts at: 0 785 1101
Size: 785 316
EarI CTCTTCn'nnn_
Cuts at: 0 138 827 1101
Size: 138 689 274
Fragments arranged by size:
689 274 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 1101
Size: 1008 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 556 1101
Size: 437 119 545
Fragments arranged by size:
545 437 119
EcoO109I rG'GnC_Cy
Cuts at: 0 355 1101
Size: 355 746
FspI TGC'GCA
Cuts at: 0 740 1101
Size: 740 361
HaeII r_GCGC'y
Cuts at: 0 1013 1101
Size: 1013 88
KpnI G_GTAC'C
Cuts at: 0 869 1101
Size: 869 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 976 1101
Size: 238 738 125
Fragments arranged by size:
738 238 125
MslI CAynn'nnrTG
Cuts at: 0 799 1101
Size: 799 302
MspA1I CmG'CkG
Cuts at: 0 784 790 1101
Size: 784 6 311
Fragments arranged by size:
784 311 6
NotI GC'GGCC_GC
Cuts at: 0 785 1101
Size: 785 316
NspI r_CATG'y
Cuts at: 0 80 1101
Size: 80 1021
PfoI T'CCnGG_A
Cuts at: 0 315 468 1101
Size: 315 153 633
Fragments arranged by size:
633 315 153
PpuMI rG'GwC_Cy
Cuts at: 0 355 1101
Size: 355 746
PstI C_TGCA'G
Cuts at: 0 58 279 1035 1101
Size: 58 221 756 66
Fragments arranged by size:
756 221 66 58
SacII CC_GC'GG
Cuts at: 0 785 791 1101
Size: 785 6 310
Fragments arranged by size:
785 310 6
SapI GCTCTTCn'nnn_
Cuts at: 0 827 1101
Size: 827 274
SfcI C'TryA_G
Cuts at: 0 54 275 350 1031 1101
Size: 54 221 75 681 70
Fragments arranged by size:
681 221 75 70 54
SmaI CCC'GGG
Cuts at: 0 494 574 1101
Size: 494 80 527
Fragments arranged by size:
527 494 80
SspI AAT'ATT
Cuts at: 0 697 1101
Size: 697 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 598 1101
Size: 598 503
XmaI C'CCGG_G
Cuts at: 0 492 572 1101
Size: 492 80 529
Fragments arranged by size:
529 492 80
Enzymes that do cut:
Acc65I AclI AfeI AgeI AlfI ApoI AseI AvaI
BanI BanII BcgI BglI Bme1580I BmrI BpmI BsaI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BstAPI BstXI BtgI EaeI EagI EarI EciI
Eco57MI EcoO109I FspI HaeII KpnI MmeI MslI MspA1I
NotI NspI PfoI PpuMI PstI SacII SapI SfcI
SmaI SspI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AcuI AflII AflIII AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaXI BseRI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI