Restriction map for
pFN21AE5554
(Linear) (Six-base) MAPSORT of: KIEE5554.seq Check: 1980 from: 1 to: 1287
KIEE5554.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 1287
Size: 48 1239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1287
Size: 239 1048
AhdI GACnn_n'nnGTC
Cuts at: 0 652 1287
Size: 652 635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 1287
Size: 35 32 1220
Fragments arranged by size:
1220 35 32
AlwNI CAG_nnn'CTG
Cuts at: 0 63 73 877 1063 1287
Size: 63 10 804 186 224
Fragments arranged by size:
804 224 186 63 10
ApaI G_GGCC'C
Cuts at: 0 375 752 844 1287
Size: 375 377 92 443
Fragments arranged by size:
443 377 375 92
ApoI r'AATT_y
Cuts at: 0 917 1287
Size: 917 370
AvaI C'yCGr_G
Cuts at: 0 21 985 1133 1287
Size: 21 964 148 154
Fragments arranged by size:
964 154 148 21
BanI G'GyrC_C
Cuts at: 0 449 702 1287
Size: 449 253 585
Fragments arranged by size:
585 449 253
BanII G_rGCy'C
Cuts at: 0 134 375 752 844 1287
Size: 134 241 377 92 443
Fragments arranged by size:
443 377 241 134 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 1287
Size: 338 949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 214 248 826 860 1287
Size: 214 34 578 34 427
Fragments arranged by size:
578 427 214 34 34
Bme1580I G_kGCm'C
Cuts at: 0 375 752 844 1287
Size: 375 377 92 443
Fragments arranged by size:
443 377 375 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 403 865 917 1287
Size: 403 462 52 370
Fragments arranged by size:
462 403 370 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 1287
Size: 93 1194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1085 1287
Size: 642 443 202
Fragments arranged by size:
642 443 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 1287
Size: 289 998
BsaHI Gr'CG_yC
Cuts at: 0 827 992 1287
Size: 827 165 295
Fragments arranged by size:
827 295 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 674 704 1287
Size: 674 30 583
Fragments arranged by size:
674 583 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1064 1287
Size: 1064 223
BseYI C'CCAG_C
Cuts at: 0 95 269 795 1159 1287
Size: 95 174 526 364 128
Fragments arranged by size:
526 364 174 128 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1287
Size: 811 476
BsiHKAI G_wGCw'C
Cuts at: 0 134 1287
Size: 134 1153
BsmI GAATG_Cn'
Cuts at: 0 115 1287
Size: 115 1172
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 1287
Size: 777 510
Bsp1286I G_dGCh'C
Cuts at: 0 134 375 752 844 1287
Size: 134 241 377 92 443
Fragments arranged by size:
443 377 241 134 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1081 1287
Size: 48 1033 206
Fragments arranged by size:
1033 206 48
BsrBI CCG'CTC
Cuts at: 0 771 1287
Size: 771 516
BsrDI GCAATG_nn'
Cuts at: 0 596 813 1287
Size: 596 217 474
Fragments arranged by size:
596 474 217
BsrFI r'CCGG_y
Cuts at: 0 676 889 1287
Size: 676 213 398
Fragments arranged by size:
676 398 213
BsrGI T'GTAC_A
Cuts at: 0 431 1287
Size: 431 856
BssSI C'ACGA_G
Cuts at: 0 1258 1287
Size: 1258 29
Bsu36I CC'TnA_GG
Cuts at: 0 941 1287
Size: 941 346
BtgI C'CryG_G
Cuts at: 0 822 1287
Size: 822 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 366 1287
Size: 366 921
BtsI GCAGTG_nn'
Cuts at: 0 881 1287
Size: 881 406
DraIII CAC_nnn'GTG
Cuts at: 0 197 709 1242 1287
Size: 197 512 533 45
Fragments arranged by size:
533 512 197 45
EarI CTCTTCn'nnn_
Cuts at: 0 436 510 1287
Size: 436 74 777
Fragments arranged by size:
777 436 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 663 1287
Size: 663 624
EcoICRI GAG'CTC
Cuts at: 0 132 1287
Size: 132 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 642 1085 1287
Size: 239 403 443 202
Fragments arranged by size:
443 403 239 202
EcoO109I rG'GnC_Cy
Cuts at: 0 229 266 371 372 728 748 749 840
Size: 229 37 105 1 356 20 1 91
Cuts at: 840 841 1287
Size: 1 446
Fragments arranged by size:
446 356 229 105 91 37 20 1
1 1
EcoRI G'AATT_C
Cuts at: 0 917 1287
Size: 917 370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 55 1287
Size: 55 1232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 638 670 1099 1131 1237 1269
Size: 457 32 149 32 429 32 106 32
Cuts at: 1269 1287
Size: 18
Fragments arranged by size:
457 429 149 106 32 32 32 32
18
HindIII A'AGCT_T
Cuts at: 0 899 1287
Size: 899 388
MslI CAynn'nnrTG
Cuts at: 0 763 806 1287
Size: 763 43 481
Fragments arranged by size:
763 481 43
MspA1I CmG'CkG
Cuts at: 0 99 587 795 1287
Size: 99 488 208 492
Fragments arranged by size:
492 488 208 99
NaeI GCC'GGC
Cuts at: 0 891 1287
Size: 891 396
NgoMIV G'CCGG_C
Cuts at: 0 889 1287
Size: 889 398
NspI r_CATG'y
Cuts at: 0 280 1287
Size: 280 1007
PasI CC'CwG_GG
Cuts at: 0 743 1030 1287
Size: 743 287 257
Fragments arranged by size:
743 287 257
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 482 587 1242 1287
Size: 393 89 105 655 45
Fragments arranged by size:
655 393 105 89 45
PfoI T'CCnGG_A
Cuts at: 0 927 1287
Size: 927 360
PpuMI rG'GwC_Cy
Cuts at: 0 229 728 1287
Size: 229 499 559
Fragments arranged by size:
559 499 229
PshAI GACnn'nnGTC
Cuts at: 0 359 1091 1142 1287
Size: 359 732 51 145
Fragments arranged by size:
732 359 145 51
PspOMI G'GGCC_C
Cuts at: 0 371 748 840 1287
Size: 371 377 92 447
Fragments arranged by size:
447 377 371 92
PstI C_TGCA'G
Cuts at: 0 59 237 1227 1287
Size: 59 178 990 60
Fragments arranged by size:
990 178 60 59
PvuII CAG'CTG
Cuts at: 0 99 587 795 1287
Size: 99 488 208 492
Fragments arranged by size:
492 488 208 99
SacI G_AGCT'C
Cuts at: 0 134 1287
Size: 134 1153
SanDI GG'GwC_CC
Cuts at: 0 728 1287
Size: 728 559
SapI GCTCTTCn'nnn_
Cuts at: 0 436 510 1287
Size: 436 74 777
Fragments arranged by size:
777 436 74
SbfI CC_TGCA'GG
Cuts at: 0 59 1287
Size: 59 1228
SexAI A'CCwGG_T
Cuts at: 0 193 705 1287
Size: 193 512 582
Fragments arranged by size:
582 512 193
SfcI C'TryA_G
Cuts at: 0 55 233 339 1223 1287
Size: 55 178 106 884 64
Fragments arranged by size:
884 178 106 64 55
SmaI CCC'GGG
Cuts at: 0 23 1287
Size: 23 1264
SmlI C'TyrA_G
Cuts at: 0 985 1133 1287
Size: 985 148 154
Fragments arranged by size:
985 154 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 1287
Size: 135 1152
TatI w'GTAC_w
Cuts at: 0 431 1287
Size: 431 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 1287
Size: 674 32 581
Fragments arranged by size:
674 581 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 856 1287
Size: 198 658 431
Fragments arranged by size:
658 431 198
XhoI C'TCGA_G
Cuts at: 0 985 1133 1287
Size: 985 148 154
Fragments arranged by size:
985 154 148
XmaI C'CCGG_G
Cuts at: 0 21 1287
Size: 21 1266
Enzymes that do cut:
AarI AcuI AhdI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BcgI Bme1580I BmrI BplI BpmI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
Bsu36I BtgI BtgZI BtsI DraIII EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SmaI SmlI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BspHI BssHII BstAPI BstBI BstEII
BstXI BstYI BstZ17I ClaI CspCI DraI DrdI EaeI
EagI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XmnI ZraI