Restriction map for
pFN21AE5552
(Linear) (Six-base) MAPSORT of: KIEE5552.seq Check: 3807 from: 1 to: 1251
KIEE5552.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AatII G_ACGT'C
Cuts at: 0 8 1251
Size: 8 1243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 398 791 1103 1251
Size: 170 228 393 312 148
Fragments arranged by size:
393 312 228 170 148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 174 206 1251
Size: 174 32 1045
Fragments arranged by size:
1045 174 32
AlwNI CAG_nnn'CTG
Cuts at: 0 193 1251
Size: 193 1058
ApoI r'AATT_y
Cuts at: 0 358 471 606 800 1251
Size: 358 113 135 194 451
Fragments arranged by size:
451 358 194 135 113
BamHI G'GATC_C
Cuts at: 0 1153 1251
Size: 1153 98
BanII G_rGCy'C
Cuts at: 0 679 1251
Size: 679 572
BbvCI CC'TCA_GC
Cuts at: 0 719 1251
Size: 719 532
BciVI GTATCCnnnnn_n'
Cuts at: 0 298 1251
Size: 298 953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 125 178 1193 1251
Size: 1 124 53 1015 58
Fragments arranged by size:
1015 124 58 53 1
Bpu10I CC'TnA_GC
Cuts at: 0 194 719 1251
Size: 194 525 532
Fragments arranged by size:
532 525 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1251
Size: 1040 211
BsaHI Gr'CG_yC
Cuts at: 0 5 1251
Size: 5 1246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 174 204 1251
Size: 174 30 1047
Fragments arranged by size:
1047 174 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 637 1251
Size: 383 254 614
Fragments arranged by size:
614 383 254
BsiHKAI G_wGCw'C
Cuts at: 0 589 1251
Size: 589 662
Bsp1286I G_dGCh'C
Cuts at: 0 589 679 1251
Size: 589 90 572
Fragments arranged by size:
589 572 90
BsrGI T'GTAC_A
Cuts at: 0 886 1251
Size: 886 365
BstYI r'GATC_y
Cuts at: 0 327 1153 1251
Size: 327 826 98
Fragments arranged by size:
826 327 98
BtgI C'CryG_G
Cuts at: 0 1196 1251
Size: 1196 55
DraI TTT'AAA
Cuts at: 0 135 440 1251
Size: 135 305 811
Fragments arranged by size:
811 305 135
EarI CTCTTCn'nnn_
Cuts at: 0 37 648 887 899 908 920 1004 1251
Size: 37 611 239 12 9 12 84 247
Fragments arranged by size:
611 247 239 84 37 12 12 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 125 170 178 398 791 1103 1193
Size: 1 124 45 8 220 393 312 90
Cuts at: 1193 1251
Size: 58
Fragments arranged by size:
393 312 220 124 90 58 45 8
1
EcoO109I rG'GnC_Cy
Cuts at: 0 205 1102 1251
Size: 205 897 149
Fragments arranged by size:
897 205 149
HaeII r_GCGC'y
Cuts at: 0 28 1251
Size: 28 1223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 938 970 1251
Size: 938 32 281
Fragments arranged by size:
938 281 32
HincII GTy'rAC
Cuts at: 0 323 1251
Size: 323 928
HpaI GTT'AAC
Cuts at: 0 323 1251
Size: 323 928
MslI CAynn'nnrTG
Cuts at: 0 698 1251
Size: 698 553
NcoI C'CATG_G
Cuts at: 0 1196 1251
Size: 1196 55
PfoI T'CCnGG_A
Cuts at: 0 17 71 465 1251
Size: 17 54 394 786
Fragments arranged by size:
786 394 54 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1251
Size: 174 32 1045
Fragments arranged by size:
1045 174 32
PpuMI rG'GwC_Cy
Cuts at: 0 205 1251
Size: 205 1046
SmlI C'TyrA_G
Cuts at: 0 1055 1251
Size: 1055 196
StyI C'CwwG_G
Cuts at: 0 331 1196 1251
Size: 331 865 55
Fragments arranged by size:
865 331 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 534 1251
Size: 534 717
TatI w'GTAC_w
Cuts at: 0 886 1251
Size: 886 365
XmnI GAAnn'nnTTC
Cuts at: 0 230 1251
Size: 230 1021
ZraI GAC'GTC
Cuts at: 0 6 1251
Size: 6 1245
Enzymes that do cut:
AatII AcuI AloI AlwNI ApoI BamHI BanII BbvCI
BciVI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsiHKAI
Bsp1286I BsrGI BstYI BtgI DraI EarI Eco57MI EcoO109I
HaeII Hin4I HincII HpaI MslI NcoI PfoI PpiI
PpuMI SmlI StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI