(Linear) (Six-base) MAPSORT of: KIEE5551.seq  Check: 2636  from: 1  to: 1233



KIEE5551.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      60    1233 
   Size:         60    1173
 
AccI GT'mk_AC

Cuts at:      0     455    1140    1233 
   Size:        455     685      93

  Fragments arranged by size: 

                 685     455      93
 
AflIII A'CryG_T

Cuts at:      0    1109    1233 
   Size:       1109     124
 
AleI CACnn'nnGTG

Cuts at:      0      74    1233 
   Size:         74    1159
 
ApoI r'AATT_y

Cuts at:      0      25      79     224     754    1187    1201    1233 
   Size:         25      54     145     530     433      14      32

  Fragments arranged by size: 

                 530     433     145      54      32      25      14
 
AseI AT'TA_AT

Cuts at:      0    1000    1004    1233 
   Size:       1000       4     229

  Fragments arranged by size: 

                1000     229       4
 
BanI G'GyrC_C

Cuts at:      0      48    1233 
   Size:         48    1185
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     842    1233 
   Size:        842     391
 
BclI T'GATC_A

Cuts at:      0     528    1233 
   Size:        528     705
 
BglII A'GATC_T

Cuts at:      0     597    1233 
   Size:        597     636
 
Bme1580I G_kGCm'C

Cuts at:      0     666    1233 
   Size:        666     567
 
BmgBI CAC'GTC

Cuts at:      0    1112    1233 
   Size:       1112     121
 
Bpu10I CC'TnA_GC

Cuts at:      0     488    1233 
   Size:        488     745
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578    1233 
   Size:        578     655
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58    1233 
   Size:         58    1175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1233 
   Size:       1066     167
 
Bsp1286I G_dGCh'C

Cuts at:      0     666    1233 
   Size:        666     567
 
BspHI T'CATG_A

Cuts at:      0     518    1233 
   Size:        518     715
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60    1233 
   Size:         60    1173
 
BstBI TT'CG_AA

Cuts at:      0    1060    1233 
   Size:       1060     173
 
BstYI r'GATC_y

Cuts at:      0     597    1233 
   Size:        597     636
 
BtsI GCAGTG_nn'

Cuts at:      0     474    1233 
   Size:        474     759
 
DraI TTT'AAA

Cuts at:      0      30      84    1233 
   Size:         30      54    1149

  Fragments arranged by size: 

                1149      54      30
 
EarI CTCTTCn'nnn_

Cuts at:      0    1062    1233 
   Size:       1062     171
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     401     954    1233 
   Size:        401     553     279

  Fragments arranged by size: 

                 553     401     279
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     513     545     836     868    1233 
   Size:        513      32     291      32     365

  Fragments arranged by size: 

                 513     365     291      32      32
 
MfeI C'AATT_G

Cuts at:      0     401    1083    1233 
   Size:        401     682     150

  Fragments arranged by size: 

                 682     401     150
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     774    1233 
   Size:        774     459
 
MslI CAynn'nnrTG

Cuts at:      0      74     177    1233 
   Size:         74     103    1056

  Fragments arranged by size: 

                1056     103      74
 
NdeI CA'TA_TG

Cuts at:      0     174    1233 
   Size:        174    1059
 
PacI TTA_AT'TAA

Cuts at:      0    1004    1233 
   Size:       1004     229
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     373    1233 
   Size:        373     860
 
PshAI GACnn'nnGTC

Cuts at:      0    1085    1233 
   Size:       1085     148
 
PsiI TTA'TAA

Cuts at:      0     859    1233 
   Size:        859     374
 
ScaI AGT'ACT

Cuts at:      0     948    1233 
   Size:        948     285
 
SfcI C'TryA_G

Cuts at:      0     390    1233 
   Size:        390     843
 
SmlI C'TyrA_G

Cuts at:      0     593    1233 
   Size:        593     640
 
SspI AAT'ATT

Cuts at:      0     686    1233 
   Size:        686     547
 
StyI C'CwwG_G

Cuts at:      0     569    1233 
   Size:        569     664
 
TatI w'GTAC_w

Cuts at:      0     946    1233 
   Size:        946     287
 
XbaI T'CTAG_A

Cuts at:      0     423    1233 
   Size:        423     810
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1097    1233 
   Size:       1097     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     963    1233 
   Size:        963     270
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AleI     ApoI     AseI     BanI    BciVI
     BclI    BglII Bme1580I    BmgBI   Bpu10I    BpuEI    BseRI     BsgI
 Bsp1286I    BspHI    BspMI    BstBI    BstYI     BtsI     DraI     EarI
     FalI    Hin4I     MfeI     MmeI     MslI     NdeI     PacI    PflMI
    PshAI     PsiI     ScaI     SfcI     SmlI     SspI     StyI     TatI
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BfrBI     BglI     BlpI     BmrI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XhoI     XmaI
     ZraI