Restriction map for
pFN21AE5547
(Linear) (Six-base) MAPSORT of: KIEE5547.seq Check: 3252 from: 1 to: 1197
KIEE5547.orf
With 182 enzymes: SgfI *
January 29, 2014 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1188 1197
Size: 1188 9
AccI GT'mk_AC
Cuts at: 0 995 1197
Size: 995 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 520 1197
Size: 374 146 677
Fragments arranged by size:
677 374 146
AflIII A'CryG_T
Cuts at: 0 831 1197
Size: 831 366
AleI CACnn'nnGTG
Cuts at: 0 834 1197
Size: 834 363
AlwNI CAG_nnn'CTG
Cuts at: 0 93 1197
Size: 93 1104
ApoI r'AATT_y
Cuts at: 0 446 1197
Size: 446 751
AvaI C'yCGr_G
Cuts at: 0 56 1197
Size: 56 1141
BanI G'GyrC_C
Cuts at: 0 1176 1197
Size: 1176 21
BfrBI ATG'CAT
Cuts at: 0 690 1197
Size: 690 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 767 1197
Size: 270 497 430
Fragments arranged by size:
497 430 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1197
Size: 1162 35
Bpu10I CC'TnA_GC
Cuts at: 0 1153 1197
Size: 1153 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1197
Size: 300 897
BsaAI yAC'GTr
Cuts at: 0 832 1062 1197
Size: 832 230 135
Fragments arranged by size:
832 230 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1197
Size: 187 30 980
Fragments arranged by size:
980 187 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1197
Size: 313 884
BseYI C'CCAG_C
Cuts at: 0 862 1081 1197
Size: 862 219 116
Fragments arranged by size:
862 219 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 1197
Size: 84 1113
BsmI GAATG_Cn'
Cuts at: 0 690 763 1012 1197
Size: 690 73 249 185
Fragments arranged by size:
690 249 185 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1180 1197
Size: 1180 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1188 1197
Size: 1188 9
BsrDI GCAATG_nn'
Cuts at: 0 50 1115 1197
Size: 50 1065 82
Fragments arranged by size:
1065 82 50
BsrFI r'CCGG_y
Cuts at: 0 748 1197
Size: 748 449
BssSI C'ACGA_G
Cuts at: 0 620 1197
Size: 620 577
BstYI r'GATC_y
Cuts at: 0 624 1126 1197
Size: 624 502 71
Fragments arranged by size:
624 502 71
Bsu36I CC'TnA_GG
Cuts at: 0 637 1197
Size: 637 560
BtgI C'CryG_G
Cuts at: 0 977 1197
Size: 977 220
BtsI GCAGTG_nn'
Cuts at: 0 732 1197
Size: 732 465
EarI CTCTTCn'nnn_
Cuts at: 0 88 1197
Size: 88 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 520 1162 1197
Size: 374 146 642 35
Fragments arranged by size:
642 374 146 35
EcoO109I rG'GnC_Cy
Cuts at: 0 663 1197
Size: 663 534
EcoRV GAT'ATC
Cuts at: 0 9 1197
Size: 9 1188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 1197
Size: 294 903
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 1197
Size: 490 32 675
Fragments arranged by size:
675 490 32
HincII GTy'rAC
Cuts at: 0 141 906 1197
Size: 141 765 291
Fragments arranged by size:
765 291 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1197
Size: 533 664
MslI CAynn'nnrTG
Cuts at: 0 380 834 939 1197
Size: 380 454 105 258
Fragments arranged by size:
454 380 258 105
MspA1I CmG'CkG
Cuts at: 0 90 862 1197
Size: 90 772 335
Fragments arranged by size:
772 335 90
NcoI C'CATG_G
Cuts at: 0 977 1197
Size: 977 220
NsiI A_TGCA'T
Cuts at: 0 692 1197
Size: 692 505
NspI r_CATG'y
Cuts at: 0 690 1197
Size: 690 507
PflMI CCAn_nnn'nTGG
Cuts at: 0 1173 1197
Size: 1173 24
PfoI T'CCnGG_A
Cuts at: 0 14 1129 1197
Size: 14 1115 68
Fragments arranged by size:
1115 68 14
PmlI CAC'GTG
Cuts at: 0 832 1062 1197
Size: 832 230 135
Fragments arranged by size:
832 230 135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 1197
Size: 273 32 892
Fragments arranged by size:
892 273 32
PsiI TTA'TAA
Cuts at: 0 181 875 1197
Size: 181 694 322
Fragments arranged by size:
694 322 181
PstI C_TGCA'G
Cuts at: 0 1185 1197
Size: 1185 12
PvuII CAG'CTG
Cuts at: 0 90 862 1197
Size: 90 772 335
Fragments arranged by size:
772 335 90
SfcI C'TryA_G
Cuts at: 0 697 1181 1197
Size: 697 484 16
Fragments arranged by size:
697 484 16
SmaI CCC'GGG
Cuts at: 0 58 1197
Size: 58 1139
SmlI C'TyrA_G
Cuts at: 0 279 1197
Size: 279 918
SrfI GCCC'GGGC
Cuts at: 0 58 1197
Size: 58 1139
SspI AAT'ATT
Cuts at: 0 225 1197
Size: 225 972
StuI AGG'CCT
Cuts at: 0 899 1197
Size: 899 298
StyI C'CwwG_G
Cuts at: 0 894 977 1197
Size: 894 83 220
Fragments arranged by size:
894 220 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 970 1197
Size: 441 529 227
Fragments arranged by size:
529 441 227
TatI w'GTAC_w
Cuts at: 0 162 793 1197
Size: 162 631 404
Fragments arranged by size:
631 404 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 1197
Size: 437 32 728
Fragments arranged by size:
728 437 32
XbaI T'CTAG_A
Cuts at: 0 712 1197
Size: 712 485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 1197
Size: 1137 60
XmaI C'CCGG_G
Cuts at: 0 56 1197
Size: 56 1141
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApoI AvaI
BanI BfrBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI
BseRI BseYI BsgI BsmI BsmBI BspMI BsrDI BsrFI
BssSI BstYI Bsu36I BtgI BtsI EarI Eco57MI EcoO109I
EcoRV FalI Hin4I HincII MmeI MslI MspA1I NcoI
NsiI NspI PflMI PfoI PmlI PpiI PsiI PstI
PvuII SfcI SmaI SmlI SrfI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SwaI Tth111I XhoI XmnI ZraI