Restriction map for
pFN21AE5546
(Linear) (Six-base) MAPSORT of: KIEE5546.seq Check: 3938 from: 1 to: 1167
KIEE5546.orf
With 182 enzymes: SgfI *
December 5, 2014 13:02 ..
AflIII A'CryG_T
Cuts at: 0 839 1167
Size: 839 328
AlwNI CAG_nnn'CTG
Cuts at: 0 579 1143 1167
Size: 579 564 24
Fragments arranged by size:
579 564 24
ApaI G_GGCC'C
Cuts at: 0 49 203 1167
Size: 49 154 964
Fragments arranged by size:
964 154 49
ApaLI G'TGCA_C
Cuts at: 0 166 706 1167
Size: 166 540 461
Fragments arranged by size:
540 461 166
AscI GG'CGCG_CC
Cuts at: 0 60 1167
Size: 60 1107
AvaI C'yCGr_G
Cuts at: 0 48 1167
Size: 48 1119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 551 584 1167
Size: 551 33 583
Fragments arranged by size:
583 551 33
BanI G'GyrC_C
Cuts at: 0 267 658 1167
Size: 267 391 509
Fragments arranged by size:
509 391 267
BanII G_rGCy'C
Cuts at: 0 49 203 626 864 887 974 1167
Size: 49 154 423 238 23 87 193
Fragments arranged by size:
423 238 193 154 87 49 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 1167
Size: 579 588
BglI GCCn_nnn'nGGC
Cuts at: 0 53 1167
Size: 53 1114
Bme1580I G_kGCm'C
Cuts at: 0 49 170 203 541 710 1167
Size: 49 121 33 338 169 457
Fragments arranged by size:
457 338 169 121 49 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 1167
Size: 567 600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 516 1167
Size: 516 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1132 1167
Size: 718 414 35
Fragments arranged by size:
718 414 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 1167
Size: 292 875
BsaAI yAC'GTr
Cuts at: 0 840 1167
Size: 840 327
BsaWI w'CCGG_w
Cuts at: 0 5 1167
Size: 5 1162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 1167
Size: 97 30 1040
Fragments arranged by size:
1040 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 235 1167
Size: 193 42 932
Fragments arranged by size:
932 193 42
BseYI C'CCAG_C
Cuts at: 0 394 626 1167
Size: 394 232 541
Fragments arranged by size:
541 394 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1167
Size: 259 908
BsiHKAI G_wGCw'C
Cuts at: 0 170 710 887 1167
Size: 170 540 177 280
Fragments arranged by size:
540 280 177 170
BsiWI C'GTAC_G
Cuts at: 0 866 1167
Size: 866 301
BsmI GAATG_Cn'
Cuts at: 0 415 1167
Size: 415 752
Bsp1286I G_dGCh'C
Cuts at: 0 49 170 203 541 626 710 864 887
Size: 49 121 33 338 85 84 154 23
Cuts at: 887 974 1167
Size: 87 193
Fragments arranged by size:
338 193 154 121 87 85 84 49
33 23
BspEI T'CCGG_A
Cuts at: 0 5 1167
Size: 5 1162
BspHI T'CATG_A
Cuts at: 0 794 1167
Size: 794 373
BsrDI GCAATG_nn'
Cuts at: 0 345 1167
Size: 345 822
BsrFI r'CCGG_y
Cuts at: 0 833 1167
Size: 833 334
BssHII G'CGCG_C
Cuts at: 0 60 1167
Size: 60 1107
BssSI C'ACGA_G
Cuts at: 0 445 1167
Size: 445 722
BstEII G'GTnAC_C
Cuts at: 0 465 1167
Size: 465 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1059 1101 1167
Size: 1059 42 66
Fragments arranged by size:
1059 66 42
BtgI C'CryG_G
Cuts at: 0 140 1167
Size: 140 1027
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 95 720 1167
Size: 95 625 447
Fragments arranged by size:
625 447 95
DrdI GACnn_nn'nnGTC
Cuts at: 0 760 1033 1167
Size: 760 273 134
Fragments arranged by size:
760 273 134
EaeI y'GGCC_r
Cuts at: 0 108 363 1167
Size: 108 255 804
Fragments arranged by size:
804 255 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 564 1167
Size: 564 603
EcoICRI GAG'CTC
Cuts at: 0 885 1167
Size: 885 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1132 1167
Size: 718 414 35
Fragments arranged by size:
718 414 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 700 1167
Size: 700 467
EcoO109I rG'GnC_Cy
Cuts at: 0 199 200 725 1167
Size: 199 1 525 442
Fragments arranged by size:
525 442 199 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 980 1040 1167
Size: 140 840 60 127
Fragments arranged by size:
840 140 127 60
MslI CAynn'nnrTG
Cuts at: 0 173 338 649 1167
Size: 173 165 311 518
Fragments arranged by size:
518 311 173 165
MspA1I CmG'CkG
Cuts at: 0 123 274 1041 1167
Size: 123 151 767 126
Fragments arranged by size:
767 151 126 123
NcoI C'CATG_G
Cuts at: 0 140 1167
Size: 140 1027
NspI r_CATG'y
Cuts at: 0 459 688 1167
Size: 459 229 479
Fragments arranged by size:
479 459 229
PasI CC'CwG_GG
Cuts at: 0 1143 1167
Size: 1143 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 251 1167
Size: 251 916
PfoI T'CCnGG_A
Cuts at: 0 695 1109 1167
Size: 695 414 58
Fragments arranged by size:
695 414 58
PspOMI G'GGCC_C
Cuts at: 0 45 199 1167
Size: 45 154 968
Fragments arranged by size:
968 154 45
PstI C_TGCA'G
Cuts at: 0 27 584 1167
Size: 27 557 583
Fragments arranged by size:
583 557 27
PvuII CAG'CTG
Cuts at: 0 123 1167
Size: 123 1044
SacI G_AGCT'C
Cuts at: 0 887 1167
Size: 887 280
SexAI A'CCwGG_T
Cuts at: 0 908 1167
Size: 908 259
SfcI C'TryA_G
Cuts at: 0 23 356 580 1167
Size: 23 333 224 587
Fragments arranged by size:
587 333 224 23
SmaI CCC'GGG
Cuts at: 0 50 1167
Size: 50 1117
SphI G_CATG'C
Cuts at: 0 459 1167
Size: 459 708
StyI C'CwwG_G
Cuts at: 0 140 1094 1167
Size: 140 954 73
Fragments arranged by size:
954 140 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 243 1167
Size: 133 110 924
Fragments arranged by size:
924 133 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 898 930 1167
Size: 898 32 237
Fragments arranged by size:
898 237 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 444 610 1167
Size: 252 192 166 557
Fragments arranged by size:
557 252 192 166
XmaI C'CCGG_G
Cuts at: 0 48 1167
Size: 48 1119
Enzymes that do cut:
AflIII AlwNI ApaI ApaLI AscI AvaI BaeI BanI
BanII BciVI BglI Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BssHII BssSI
BstEII BstXI BtgI BtgZI DrdI EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I MmeI MslI MspA1I NcoI NspI
PasI PflMI PfoI PspOMI PstI PvuII SacI SexAI
SfcI SmaI SphI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApoI AseI AsiSI
AvrII BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsaHI
BsiEI BsmBI BspMI BsrBI BsrGI BstAPI BstBI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI