Restriction map for
pFN21AE5544
(Linear) (Six-base) MAPSORT of: KIEE5544.seq Check: 3309 from: 1 to: 1143
KIEE5544.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 692 1143
Size: 692 451
AccI GT'mk_AC
Cuts at: 0 1043 1143
Size: 1043 100
AflII C'TTAA_G
Cuts at: 0 1054 1143
Size: 1054 89
AflIII A'CryG_T
Cuts at: 0 331 1143
Size: 331 812
AlwNI CAG_nnn'CTG
Cuts at: 0 96 448 693 774 1143
Size: 96 352 245 81 369
Fragments arranged by size:
369 352 245 96 81
ApaI G_GGCC'C
Cuts at: 0 32 573 1143
Size: 32 541 570
Fragments arranged by size:
570 541 32
ApoI r'AATT_y
Cuts at: 0 917 1143
Size: 917 226
AvaI C'yCGr_G
Cuts at: 0 31 1085 1143
Size: 31 1054 58
Fragments arranged by size:
1054 58 31
BanII G_rGCy'C
Cuts at: 0 32 231 326 573 1143
Size: 32 199 95 247 570
Fragments arranged by size:
570 247 199 95 32
Bme1580I G_kGCm'C
Cuts at: 0 32 405 573 707 1143
Size: 32 373 168 134 436
Fragments arranged by size:
436 373 168 134 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 928 1143
Size: 93 835 215
Fragments arranged by size:
835 215 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 867 1143
Size: 867 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 598 761 898 1087 1143
Size: 28 570 163 137 189 56
Fragments arranged by size:
570 189 163 137 56 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 724 894 1143
Size: 724 170 249
Fragments arranged by size:
724 249 170
BsaAI yAC'GTr
Cuts at: 0 1034 1143
Size: 1034 109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 541 877 882 1120 1143
Size: 239 302 336 5 238 23
Fragments arranged by size:
336 302 239 238 23 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 232 341 346 1143
Size: 186 46 109 5 797
Fragments arranged by size:
797 186 109 46 5
BsiEI CG_ry'CG
Cuts at: 0 979 1143
Size: 979 164
BsiHKAI G_wGCw'C
Cuts at: 0 494 1143
Size: 494 649
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 1143
Size: 232 911
Bsp1286I G_dGCh'C
Cuts at: 0 32 231 326 405 494 573 707 1143
Size: 32 199 95 79 89 79 134 436
Fragments arranged by size:
436 199 134 95 89 79 79 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 337 659 692 1016 1143
Size: 337 322 33 324 127
Fragments arranged by size:
337 324 322 127 33
BsrFI r'CCGG_y
Cuts at: 0 974 1143
Size: 974 169
BsrGI T'GTAC_A
Cuts at: 0 644 1143
Size: 644 499
BstAPI GCAn_nnn'nTGC
Cuts at: 0 777 1143
Size: 777 366
Bsu36I CC'TnA_GG
Cuts at: 0 17 1143
Size: 17 1126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 351 1143
Size: 351 792
BtsI GCAGTG_nn'
Cuts at: 0 142 358 446 1143
Size: 142 216 88 697
Fragments arranged by size:
697 216 142 88
EaeI y'GGCC_r
Cuts at: 0 972 976 1143
Size: 972 4 167
Fragments arranged by size:
972 167 4
EagI C'GGCC_G
Cuts at: 0 976 1143
Size: 976 167
EarI CTCTTCn'nnn_
Cuts at: 0 118 1143
Size: 118 1025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 445 1143
Size: 445 698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 598 761 898 1087 1143
Size: 28 570 163 137 189 56
Fragments arranged by size:
570 189 163 137 56 28
EcoO109I rG'GnC_Cy
Cuts at: 0 28 569 1143
Size: 28 541 574
Fragments arranged by size:
574 541 28
FseI GG_CCGG'CC
Cuts at: 0 978 1143
Size: 978 165
HaeII r_GCGC'y
Cuts at: 0 247 1143
Size: 247 896
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 548 580 853 885 1143
Size: 548 32 273 32 258
Fragments arranged by size:
548 273 258 32 32
HindIII A'AGCT_T
Cuts at: 0 103 1143
Size: 103 1040
MslI CAynn'nnrTG
Cuts at: 0 760 1143
Size: 760 383
NaeI GCC'GGC
Cuts at: 0 976 1143
Size: 976 167
NdeI CA'TA_TG
Cuts at: 0 110 1143
Size: 110 1033
NgoMIV G'CCGG_C
Cuts at: 0 974 1143
Size: 974 169
NspI r_CATG'y
Cuts at: 0 335 1143
Size: 335 808
PasI CC'CwG_GG
Cuts at: 0 231 582 1143
Size: 231 351 561
Fragments arranged by size:
561 351 231
PciI A'CATG_T
Cuts at: 0 331 1143
Size: 331 812
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 921 1143
Size: 96 825 222
Fragments arranged by size:
825 222 96
PfoI T'CCnGG_A
Cuts at: 0 875 1143
Size: 875 268
PspOMI G'GGCC_C
Cuts at: 0 28 569 1143
Size: 28 541 574
Fragments arranged by size:
574 541 28
PstI C_TGCA'G
Cuts at: 0 119 689 1143
Size: 119 570 454
Fragments arranged by size:
570 454 119
SexAI A'CCwGG_T
Cuts at: 0 392 1143
Size: 392 751
SfcI C'TryA_G
Cuts at: 0 115 685 1117 1143
Size: 115 570 432 26
Fragments arranged by size:
570 432 115 26
SmaI CCC'GGG
Cuts at: 0 33 1143
Size: 33 1110
SmlI C'TyrA_G
Cuts at: 0 1054 1085 1143
Size: 1054 31 58
Fragments arranged by size:
1054 58 31
StyI C'CwwG_G
Cuts at: 0 158 284 1143
Size: 158 126 859
Fragments arranged by size:
859 158 126
TatI w'GTAC_w
Cuts at: 0 644 1143
Size: 644 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1143
Size: 484 32 627
Fragments arranged by size:
627 484 32
XbaI T'CTAG_A
Cuts at: 0 132 1143
Size: 132 1011
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 805 1143
Size: 805 338
XhoI C'TCGA_G
Cuts at: 0 1085 1143
Size: 1085 58
XmaI C'CCGG_G
Cuts at: 0 31 1143
Size: 31 1112
Enzymes that do cut:
AarI AccI AflII AflIII AlwNI ApaI ApoI AvaI
BanII Bme1580I BmrI BplI BpmI BsaI BsaAI BseRI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BstAPI Bsu36I BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoO109I FseI HaeII Hin4I HindIII MslI NaeI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PspOMI
PstI SexAI SfcI SmaI SmlI StyI TatI TstI
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NarI NcoI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XmnI ZraI