Restriction map for
pFN21AE5542
(Linear) (Six-base) MAPSORT of: KIEE5542.seq Check: 6997 from: 1 to: 1095
KIEE5542.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1054 1095
Size: 1054 41
Acc65I G'GTAC_C
Cuts at: 0 1028 1095
Size: 1028 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 646 1095
Size: 472 174 449
Fragments arranged by size:
472 449 174
AflIII A'CryG_T
Cuts at: 0 1022 1095
Size: 1022 73
ApoI r'AATT_y
Cuts at: 0 90 468 902 1095
Size: 90 378 434 193
Fragments arranged by size:
434 378 193 90
AvaI C'yCGr_G
Cuts at: 0 1058 1095
Size: 1058 37
BanI G'GyrC_C
Cuts at: 0 1028 1095
Size: 1028 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 450 1095
Size: 416 34 645
Fragments arranged by size:
645 416 34
BglI GCCn_nnn'nGGC
Cuts at: 0 737 1095
Size: 737 358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 610 1095
Size: 352 258 485
Fragments arranged by size:
485 352 258
Bpu10I CC'TnA_GC
Cuts at: 0 476 1095
Size: 476 619
BsaI GGTCTCn'nnnn_
Cuts at: 0 817 1095
Size: 817 278
BsaWI w'CCGG_w
Cuts at: 0 126 1095
Size: 126 969
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 583 613 952 982 991 1021
Size: 331 30 222 30 339 30 9 30
Cuts at: 1021 1095
Size: 74
Fragments arranged by size:
339 331 222 74 30 30 30 30
9
BseYI C'CCAG_C
Cuts at: 0 350 1095
Size: 350 745
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 1095
Size: 684 411
BsiEI CG_ry'CG
Cuts at: 0 741 1095
Size: 741 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 929 1054 1095
Size: 929 125 41
Fragments arranged by size:
929 125 41
BsrDI GCAATG_nn'
Cuts at: 0 979 1095
Size: 979 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 852 869 1095
Size: 852 17 226
Fragments arranged by size:
852 226 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 523 1017 1095
Size: 523 494 78
Fragments arranged by size:
523 494 78
BstYI r'GATC_y
Cuts at: 0 817 1095
Size: 817 278
BtsI GCAGTG_nn'
Cuts at: 0 117 1095
Size: 117 978
EaeI y'GGCC_r
Cuts at: 0 738 1095
Size: 738 357
EagI C'GGCC_G
Cuts at: 0 738 1095
Size: 738 357
EarI CTCTTCn'nnn_
Cuts at: 0 629 1095
Size: 629 466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 1095
Size: 21 1074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 472 610 646 1095
Size: 352 120 138 36 449
Fragments arranged by size:
449 352 138 120 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1061 1095
Size: 1061 34
EcoRI G'AATT_C
Cuts at: 0 90 1095
Size: 90 1005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 825 1095
Size: 825 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 555 587 991 1023 1095
Size: 469 32 54 32 404 32 72
Fragments arranged by size:
469 404 72 54 32 32 32
HincII GTy'rAC
Cuts at: 0 768 1095
Size: 768 327
KpnI G_GTAC'C
Cuts at: 0 1032 1095
Size: 1032 63
MfeI C'AATT_G
Cuts at: 0 105 1095
Size: 105 990
MslI CAynn'nnrTG
Cuts at: 0 1015 1095
Size: 1015 80
MspA1I CmG'CkG
Cuts at: 0 519 1095
Size: 519 576
NspI r_CATG'y
Cuts at: 0 161 1026 1095
Size: 161 865 69
Fragments arranged by size:
865 161 69
PciI A'CATG_T
Cuts at: 0 1022 1095
Size: 1022 73
PfoI T'CCnGG_A
Cuts at: 0 704 1095
Size: 704 391
PsiI TTA'TAA
Cuts at: 0 212 1095
Size: 212 883
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 322 354 1095
Size: 322 32 741
Fragments arranged by size:
741 322 32
PstI C_TGCA'G
Cuts at: 0 857 926 1095
Size: 857 69 169
Fragments arranged by size:
857 169 69
PvuII CAG'CTG
Cuts at: 0 519 1095
Size: 519 576
SapI GCTCTTCn'nnn_
Cuts at: 0 629 1095
Size: 629 466
ScaI AGT'ACT
Cuts at: 0 877 1095
Size: 877 218
SfcI C'TryA_G
Cuts at: 0 853 922 1095
Size: 853 69 173
Fragments arranged by size:
853 173 69
SphI G_CATG'C
Cuts at: 0 161 1095
Size: 161 934
TatI w'GTAC_w
Cuts at: 0 875 1095
Size: 875 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 936 968 1095
Size: 583 32 321 32 127
Fragments arranged by size:
583 321 127 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 1095
Size: 585 510
XmnI GAAnn'nnTTC
Cuts at: 0 37 1095
Size: 37 1058
Enzymes that do cut:
AarI Acc65I AcuI AflIII ApoI AvaI BanI BcgI
BglI BpmI Bpu10I BsaI BsaWI BsaXI BseYI BsgI
BsiEI BspMI BsrDI BstAPI BstXI BstYI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI Hin4I
HincII KpnI MfeI MslI MspA1I NspI PciI PfoI
PsiI PsrI PstI PvuII SapI ScaI SfcI SphI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BseRI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI