Restriction map for
pFN21AE5540
(Linear) (Six-base) MAPSORT of: KIEE5540.seq Check: 688 from: 1 to: 1083
KIEE5540.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1083
Size: 898 185
AlwNI CAG_nnn'CTG
Cuts at: 0 39 118 232 360 717 789 984 1062
Size: 39 79 114 128 357 72 195 78
Cuts at: 1062 1083
Size: 21
Fragments arranged by size:
357 195 128 114 79 78 72 39
21
ApaI G_GGCC'C
Cuts at: 0 128 1083
Size: 128 955
ApoI r'AATT_y
Cuts at: 0 160 1083
Size: 160 923
AvaI C'yCGr_G
Cuts at: 0 383 586 1083
Size: 383 203 497
Fragments arranged by size:
497 383 203
BanII G_rGCy'C
Cuts at: 0 128 1032 1083
Size: 128 904 51
Fragments arranged by size:
904 128 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 626 1083
Size: 626 457
BbvCI CC'TCA_GC
Cuts at: 0 512 1083
Size: 512 571
BglI GCCn_nnn'nGGC
Cuts at: 0 235 508 526 588 637 1083
Size: 235 273 18 62 49 446
Fragments arranged by size:
446 273 235 62 49 18
BglII A'GATC_T
Cuts at: 0 50 1083
Size: 50 1033
BlpI GC'TnA_GC
Cuts at: 0 188 285 1083
Size: 188 97 798
Fragments arranged by size:
798 188 97
Bme1580I G_kGCm'C
Cuts at: 0 128 1083
Size: 128 955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 739 811 1083
Size: 206 533 72 272
Fragments arranged by size:
533 272 206 72
Bpu10I CC'TnA_GC
Cuts at: 0 512 1083
Size: 512 571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1083
Size: 89 994
BsaHI Gr'CG_yC
Cuts at: 0 23 1083
Size: 23 1060
BsaWI w'CCGG_w
Cuts at: 0 164 458 1083
Size: 164 294 625
Fragments arranged by size:
625 294 164
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 808 1083
Size: 529 279 275
Fragments arranged by size:
529 279 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 1083
Size: 549 534
BsiEI CG_ry'CG
Cuts at: 0 935 1083
Size: 935 148
BsiHKAI G_wGCw'C
Cuts at: 0 194 1083
Size: 194 889
Bsp1286I G_dGCh'C
Cuts at: 0 128 194 1032 1083
Size: 128 66 838 51
Fragments arranged by size:
838 128 66 51
BspEI T'CCGG_A
Cuts at: 0 164 1083
Size: 164 919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 992 1083
Size: 38 954 91
Fragments arranged by size:
954 91 38
BsrFI r'CCGG_y
Cuts at: 0 627 707 743 914 934 968 1083
Size: 627 80 36 171 20 34 115
Fragments arranged by size:
627 171 115 80 36 34 20
BssHII G'CGCG_C
Cuts at: 0 82 1068 1083
Size: 82 986 15
Fragments arranged by size:
986 82 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 984 1083
Size: 984 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 1083
Size: 207 876
BstYI r'GATC_y
Cuts at: 0 50 371 842 1083
Size: 50 321 471 241
Fragments arranged by size:
471 321 241 50
Bsu36I CC'TnA_GG
Cuts at: 0 248 1083
Size: 248 835
BtgI C'CryG_G
Cuts at: 0 521 574 1083
Size: 521 53 509
Fragments arranged by size:
521 509 53
BtsI GCAGTG_nn'
Cuts at: 0 402 1083
Size: 402 681
EaeI y'GGCC_r
Cuts at: 0 41 311 629 762 932 1083
Size: 41 270 318 133 170 151
Fragments arranged by size:
318 270 170 151 133 41
EagI C'GGCC_G
Cuts at: 0 932 1083
Size: 932 151
EarI CTCTTCn'nnn_
Cuts at: 0 170 460 652 1083
Size: 170 290 192 431
Fragments arranged by size:
431 290 192 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 453 844 1083
Size: 453 391 239
Fragments arranged by size:
453 391 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 739 811 898 1083
Size: 206 533 72 87 185
Fragments arranged by size:
533 206 185 87 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 823 865 1054 1083
Size: 823 42 189 29
Fragments arranged by size:
823 189 42 29
EcoO109I rG'GnC_Cy
Cuts at: 0 124 125 560 873 1059 1083
Size: 124 1 435 313 186 24
Fragments arranged by size:
435 313 186 124 24 1
FspI TGC'GCA
Cuts at: 0 988 1083
Size: 988 95
HaeII r_GCGC'y
Cuts at: 0 726 915 963 1017 1083
Size: 726 189 48 54 66
Fragments arranged by size:
726 189 66 54 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1083
Size: 310 32 741
Fragments arranged by size:
741 310 32
MscI TGG'CCA
Cuts at: 0 43 313 1083
Size: 43 270 770
Fragments arranged by size:
770 270 43
MspA1I CmG'CkG
Cuts at: 0 576 690 714 906 1083
Size: 576 114 24 192 177
Fragments arranged by size:
576 192 177 114 24
NaeI GCC'GGC
Cuts at: 0 629 745 936 1083
Size: 629 116 191 147
Fragments arranged by size:
629 191 147 116
NgoMIV G'CCGG_C
Cuts at: 0 627 743 934 1083
Size: 627 116 191 149
Fragments arranged by size:
627 191 149 116
PflMI CCAn_nnn'nTGG
Cuts at: 0 537 1083
Size: 537 546
PfoI T'CCnGG_A
Cuts at: 0 374 1083
Size: 374 709
PpuMI rG'GwC_Cy
Cuts at: 0 1059 1083
Size: 1059 24
PspOMI G'GGCC_C
Cuts at: 0 124 1083
Size: 124 959
PstI C_TGCA'G
Cuts at: 0 35 123 303 731 869 1083
Size: 35 88 180 428 138 214
Fragments arranged by size:
428 214 180 138 88 35
PvuII CAG'CTG
Cuts at: 0 714 1083
Size: 714 369
SacII CC_GC'GG
Cuts at: 0 577 1083
Size: 577 506
SbfI CC_TGCA'GG
Cuts at: 0 731 1083
Size: 731 352
SfcI C'TryA_G
Cuts at: 0 31 119 299 727 865 1083
Size: 31 88 180 428 138 218
Fragments arranged by size:
428 218 180 138 88 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 526 1083
Size: 526 557
SgrAI Cr'CCGG_yG
Cuts at: 0 914 1083
Size: 914 169
SmaI CCC'GGG
Cuts at: 0 385 588 1083
Size: 385 203 495
Fragments arranged by size:
495 385 203
SmlI C'TyrA_G
Cuts at: 0 104 1083
Size: 104 979
SrfI GCCC'GGGC
Cuts at: 0 588 1083
Size: 588 495
StuI AGG'CCT
Cuts at: 0 246 1076 1083
Size: 246 830 7
Fragments arranged by size:
830 246 7
StyI C'CwwG_G
Cuts at: 0 17 206 401 422 1083
Size: 17 189 195 21 661
Fragments arranged by size:
661 195 189 21 17
XmaI C'CCGG_G
Cuts at: 0 383 586 1083
Size: 383 203 497
Fragments arranged by size:
497 383 203
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaHI
BsaWI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MscI MspA1I NaeI NgoMIV PflMI PfoI
PpuMI PspOMI PstI PvuII SacII SbfI SfcI SfiI
SgrAI SmaI SmlI SrfI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaXI BseYI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI ScaI SexAI SfoI SgfI SnaBI
SpeI SphI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI