Restriction map for
pFN21AE5534
(Linear) (Six-base) MAPSORT of: KIEE5534.seq Check: 1994 from: 1 to: 1311
KIEE5534.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 1153 1311
Size: 330 823 158
Fragments arranged by size:
823 330 158
AccI GT'mk_AC
Cuts at: 0 1253 1311
Size: 1253 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 619 723 1311
Size: 69 550 104 588
Fragments arranged by size:
588 550 104 69
AleI CACnn'nnGTG
Cuts at: 0 686 1311
Size: 686 625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 596 750 1311
Size: 596 154 561
Fragments arranged by size:
596 561 154
AlwNI CAG_nnn'CTG
Cuts at: 0 345 483 1311
Size: 345 138 828
Fragments arranged by size:
828 345 138
ApaI G_GGCC'C
Cuts at: 0 545 1169 1237 1311
Size: 545 624 68 74
Fragments arranged by size:
624 545 74 68
ApoI r'AATT_y
Cuts at: 0 838 1311
Size: 838 473
AvaI C'yCGr_G
Cuts at: 0 213 1236 1311
Size: 213 1023 75
Fragments arranged by size:
1023 213 75
BamHI G'GATC_C
Cuts at: 0 9 98 1311
Size: 9 89 1213
Fragments arranged by size:
1213 89 9
BanII G_rGCy'C
Cuts at: 0 545 953 1169 1237 1311
Size: 545 408 216 68 74
Fragments arranged by size:
545 408 216 74 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 607 1311
Size: 607 704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 314 1311
Size: 280 34 997
Fragments arranged by size:
997 280 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 79 1311
Size: 79 1232
BclI T'GATC_A
Cuts at: 0 21 1311
Size: 21 1290
BlpI GC'TnA_GC
Cuts at: 0 1304 1311
Size: 1304 7
Bme1580I G_kGCm'C
Cuts at: 0 545 1169 1237 1311
Size: 545 624 68 74
Fragments arranged by size:
624 545 74 68
BmgBI CAC'GTC
Cuts at: 0 684 1311
Size: 684 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 802 1311
Size: 723 79 509
Fragments arranged by size:
723 509 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 648 1311
Size: 648 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1311
Size: 941 370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1311
Size: 519 792
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 570 1269 1311
Size: 73 497 699 42
Fragments arranged by size:
699 497 73 42
BsaBI GATnn'nnATC
Cuts at: 0 103 829 1311
Size: 103 726 482
Fragments arranged by size:
726 482 103
BsaHI Gr'CG_yC
Cuts at: 0 1036 1311
Size: 1036 275
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 640 961 1311
Size: 280 360 321 350
Fragments arranged by size:
360 350 321 280
BseYI C'CCAG_C
Cuts at: 0 1214 1311
Size: 1214 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1136 1311
Size: 556 580 175
Fragments arranged by size:
580 556 175
BsiHKAI G_wGCw'C
Cuts at: 0 272 953 1243 1311
Size: 272 681 290 68
Fragments arranged by size:
681 290 272 68
Bsp1286I G_dGCh'C
Cuts at: 0 272 545 953 1169 1237 1243 1311
Size: 272 273 408 216 68 6 68
Fragments arranged by size:
408 273 272 216 68 68 6
BspHI T'CATG_A
Cuts at: 0 878 1311
Size: 878 433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 645 1153 1311
Size: 330 315 508 158
Fragments arranged by size:
508 330 315 158
BsrDI GCAATG_nn'
Cuts at: 0 1074 1311
Size: 1074 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1064 1078 1311
Size: 1064 14 233
Fragments arranged by size:
1064 233 14
BstYI r'GATC_y
Cuts at: 0 9 98 480 1283 1311
Size: 9 89 382 803 28
Fragments arranged by size:
803 382 89 28 9
BstZ17I GTA'TAC
Cuts at: 0 1254 1311
Size: 1254 57
BtgI C'CryG_G
Cuts at: 0 74 1311
Size: 74 1237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 855 1311
Size: 855 456
BtsI GCAGTG_nn'
Cuts at: 0 308 334 732 1311
Size: 308 26 398 579
Fragments arranged by size:
579 398 308 26
DraIII CAC_nnn'GTG
Cuts at: 0 1064 1311
Size: 1064 247
EaeI y'GGCC_r
Cuts at: 0 353 818 1311
Size: 353 465 493
Fragments arranged by size:
493 465 353
EarI CTCTTCn'nnn_
Cuts at: 0 586 1311
Size: 586 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 1311
Size: 301 1010
EcoICRI GAG'CTC
Cuts at: 0 951 1311
Size: 951 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 619 723 941 1311
Size: 69 550 104 218 370
Fragments arranged by size:
550 370 218 104 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 319 1311
Size: 157 162 992
Fragments arranged by size:
992 162 157
EcoO109I rG'GnC_Cy
Cuts at: 0 295 542 717 1166 1233 1311
Size: 295 247 175 449 67 78
Fragments arranged by size:
449 295 247 175 78 67
EcoRI G'AATT_C
Cuts at: 0 838 1311
Size: 838 473
FspI TGC'GCA
Cuts at: 0 58 1311
Size: 58 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 683 715 1311
Size: 683 32 596
Fragments arranged by size:
683 596 32
MscI TGG'CCA
Cuts at: 0 820 1311
Size: 820 491
MslI CAynn'nnrTG
Cuts at: 0 142 160 686 752 1311
Size: 142 18 526 66 559
Fragments arranged by size:
559 526 142 66 18
MspA1I CmG'CkG
Cuts at: 0 225 1311
Size: 225 1086
NcoI C'CATG_G
Cuts at: 0 74 1311
Size: 74 1237
NspI r_CATG'y
Cuts at: 0 528 1198 1311
Size: 528 670 113
Fragments arranged by size:
670 528 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1311
Size: 483 828
PfoI T'CCnGG_A
Cuts at: 0 701 1124 1311
Size: 701 423 187
Fragments arranged by size:
701 423 187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 1311
Size: 355 32 924
Fragments arranged by size:
924 355 32
PpuMI rG'GwC_Cy
Cuts at: 0 717 1311
Size: 717 594
PspOMI G'GGCC_C
Cuts at: 0 541 1165 1233 1311
Size: 541 624 68 78
Fragments arranged by size:
624 541 78 68
PstI C_TGCA'G
Cuts at: 0 303 341 890 1034 1311
Size: 303 38 549 144 277
Fragments arranged by size:
549 303 277 144 38
PvuII CAG'CTG
Cuts at: 0 225 1311
Size: 225 1086
SacI G_AGCT'C
Cuts at: 0 953 1311
Size: 953 358
SapI GCTCTTCn'nnn_
Cuts at: 0 586 1311
Size: 586 725
SfcI C'TryA_G
Cuts at: 0 299 337 417 432 886 1030 1311
Size: 299 38 80 15 454 144 281
Fragments arranged by size:
454 299 281 144 80 38 15
SmlI C'TyrA_G
Cuts at: 0 534 1311
Size: 534 777
SphI G_CATG'C
Cuts at: 0 528 1311
Size: 528 783
StuI AGG'CCT
Cuts at: 0 631 1311
Size: 631 680
StyI C'CwwG_G
Cuts at: 0 74 251 320 649 1259 1311
Size: 74 177 69 329 610 52
Fragments arranged by size:
610 329 177 74 69 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 341 607 1311
Size: 341 266 704
Fragments arranged by size:
704 341 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 522 554 1311
Size: 522 32 757
Fragments arranged by size:
757 522 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 1311
Size: 1156 155
XmnI GAAnn'nnTTC
Cuts at: 0 367 1311
Size: 367 944
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanII BbsI BcgI BciVI BclI BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BstAPI BstYI BstZ17I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI Hin4I MscI MslI MspA1I NcoI NspI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SfcI SmlI SphI StuI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BfrBI BglI BglII BmtI Bpu10I BsaAI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI Bsu36I
ClaI CspCI DraI DrdI EagI EcoRV FalI FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI