Restriction map for
pFN21AE5533
(Linear) (Six-base) MAPSORT of: KIEE5533.seq Check: 5097 from: 1 to: 1401
KIEE5533.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 897 1401
Size: 897 504
ApaI G_GGCC'C
Cuts at: 0 470 1016 1401
Size: 470 546 385
Fragments arranged by size:
546 470 385
ApoI r'AATT_y
Cuts at: 0 262 328 1401
Size: 262 66 1073
Fragments arranged by size:
1073 262 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 238 271 1401
Size: 238 33 1130
Fragments arranged by size:
1130 238 33
BanI G'GyrC_C
Cuts at: 0 373 1027 1401
Size: 373 654 374
Fragments arranged by size:
654 374 373
BanII G_rGCy'C
Cuts at: 0 17 470 638 1016 1380 1401
Size: 17 453 168 378 364 21
Fragments arranged by size:
453 378 364 168 21 17
Bme1580I G_kGCm'C
Cuts at: 0 79 200 470 766 1016 1401
Size: 79 121 270 296 250 385
Fragments arranged by size:
385 296 270 250 121 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 748 1282 1401
Size: 189 559 534 119
Fragments arranged by size:
559 534 189 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 595 1401
Size: 514 81 806
Fragments arranged by size:
806 514 81
BsaHI Gr'CG_yC
Cuts at: 0 125 1401
Size: 125 1276
BsaWI w'CCGG_w
Cuts at: 0 699 1401
Size: 699 702
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 707 1063 1401
Size: 707 356 338
Fragments arranged by size:
707 356 338
BseYI C'CCAG_C
Cuts at: 0 199 1373 1401
Size: 199 1174 28
Fragments arranged by size:
1174 199 28
BsiHKAI G_wGCw'C
Cuts at: 0 17 638 699 1401
Size: 17 621 61 702
Fragments arranged by size:
702 621 61 17
BsmI GAATG_Cn'
Cuts at: 0 456 1401
Size: 456 945
Bsp1286I G_dGCh'C
Cuts at: 0 17 79 200 470 638 699 766 1016
Size: 17 62 121 270 168 61 67 250
Cuts at: 1016 1380 1401
Size: 364 21
Fragments arranged by size:
364 270 250 168 121 67 62 61
21 17
BspHI T'CATG_A
Cuts at: 0 405 1401
Size: 405 996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 835 1401
Size: 530 305 566
Fragments arranged by size:
566 530 305
BsrBI CCG'CTC
Cuts at: 0 567 1401
Size: 567 834
BsrDI GCAATG_nn'
Cuts at: 0 625 1401
Size: 625 776
BstXI CCAn_nnnn'nTGG
Cuts at: 0 869 1151 1295 1401
Size: 869 282 144 106
Fragments arranged by size:
869 282 144 106
Bsu36I CC'TnA_GG
Cuts at: 0 665 1401
Size: 665 736
BtgI C'CryG_G
Cuts at: 0 1278 1401
Size: 1278 123
BtsI GCAGTG_nn'
Cuts at: 0 837 1401
Size: 837 564
EaeI y'GGCC_r
Cuts at: 0 1230 1401
Size: 1230 171
EarI CTCTTCn'nnn_
Cuts at: 0 574 1401
Size: 574 827
EcoICRI GAG'CTC
Cuts at: 0 15 636 1401
Size: 15 621 765
Fragments arranged by size:
765 621 15
EcoO109I rG'GnC_Cy
Cuts at: 0 466 1070 1401
Size: 466 604 331
Fragments arranged by size:
604 466 331
HaeII r_GCGC'y
Cuts at: 0 92 1401
Size: 92 1309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 883 915 1401
Size: 568 32 283 32 486
Fragments arranged by size:
568 486 283 32 32
MfeI C'AATT_G
Cuts at: 0 944 1401
Size: 944 457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1401
Size: 659 742
MscI TGG'CCA
Cuts at: 0 1232 1401
Size: 1232 169
MslI CAynn'nnrTG
Cuts at: 0 867 1401
Size: 867 534
MspA1I CmG'CkG
Cuts at: 0 135 1221 1401
Size: 135 1086 180
Fragments arranged by size:
1086 180 135
NcoI C'CATG_G
Cuts at: 0 1278 1401
Size: 1278 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 347 1401
Size: 347 1054
PpuMI rG'GwC_Cy
Cuts at: 0 1070 1401
Size: 1070 331
PsiI TTA'TAA
Cuts at: 0 842 1401
Size: 842 559
PspOMI G'GGCC_C
Cuts at: 0 466 1012 1401
Size: 466 546 389
Fragments arranged by size:
546 466 389
PstI C_TGCA'G
Cuts at: 0 832 1401
Size: 832 569
PvuII CAG'CTG
Cuts at: 0 135 1221 1401
Size: 135 1086 180
Fragments arranged by size:
1086 180 135
SacI G_AGCT'C
Cuts at: 0 17 638 1401
Size: 17 621 763
Fragments arranged by size:
763 621 17
SapI GCTCTTCn'nnn_
Cuts at: 0 574 1401
Size: 574 827
SfcI C'TryA_G
Cuts at: 0 828 909 1037 1401
Size: 828 81 128 364
Fragments arranged by size:
828 364 128 81
SmlI C'TyrA_G
Cuts at: 0 493 574 1401
Size: 493 81 827
Fragments arranged by size:
827 493 81
SspI AAT'ATT
Cuts at: 0 436 1008 1401
Size: 436 572 393
Fragments arranged by size:
572 436 393
StuI AGG'CCT
Cuts at: 0 367 1401
Size: 367 1034
StyI C'CwwG_G
Cuts at: 0 1227 1278 1401
Size: 1227 51 123
Fragments arranged by size:
1227 123 51
TatI w'GTAC_w
Cuts at: 0 536 1401
Size: 536 865
Tth111I GACn'n_nGTC
Cuts at: 0 481 1135 1401
Size: 481 654 266
Fragments arranged by size:
654 481 266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1245 1401
Size: 1245 156
XmnI GAAnn'nnTTC
Cuts at: 0 557 1401
Size: 557 844
Enzymes that do cut:
AhdI ApaI ApoI BaeI BanI BanII Bme1580I BmrI
BpuEI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BstXI Bsu36I BtgI BtsI
EaeI EarI EcoICRI EcoO109I HaeII Hin4I MfeI MmeI
MscI MslI MspA1I NcoI PflMI PpuMI PsiI PspOMI
PstI PvuII SacI SapI SfcI SmlI SspI StuI
StyI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII TstI XbaI XhoI XmaI ZraI