Restriction map for
pFN21AE5532
(Linear) (Six-base) MAPSORT of: KIEE5532.seq Check: 4085 from: 1 to: 1179
KIEE5532.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1080 1179
Size: 1080 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 994 1099 1179
Size: 358 636 105 80
Fragments arranged by size:
636 358 105 80
AhdI GACnn_n'nnGTC
Cuts at: 0 861 1179
Size: 861 318
AlwNI CAG_nnn'CTG
Cuts at: 0 200 852 972 1155 1179
Size: 200 652 120 183 24
Fragments arranged by size:
652 200 183 120 24
AvaI C'yCGr_G
Cuts at: 0 215 697 1109 1179
Size: 215 482 412 70
Fragments arranged by size:
482 412 215 70
BanII G_rGCy'C
Cuts at: 0 896 1179
Size: 896 283
BbsI GAAGACnn'nnnn_
Cuts at: 0 1056 1179
Size: 1056 123
BglII A'GATC_T
Cuts at: 0 314 1179
Size: 314 865
BlpI GC'TnA_GC
Cuts at: 0 517 592 1179
Size: 517 75 587
Fragments arranged by size:
587 517 75
BmgBI CAC'GTC
Cuts at: 0 346 384 1059 1179
Size: 346 38 675 120
Fragments arranged by size:
675 346 120 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 1179
Size: 253 926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1120 1179
Size: 124 996 59
Fragments arranged by size:
996 124 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1179
Size: 205 974
BsaI GGTCTCn'nnnn_
Cuts at: 0 1134 1179
Size: 1134 45
BsaWI w'CCGG_w
Cuts at: 0 479 1179
Size: 479 700
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 907 937 1179
Size: 907 30 242
Fragments arranged by size:
907 242 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 526 1179
Size: 10 516 653
Fragments arranged by size:
653 516 10
BseYI C'CCAG_C
Cuts at: 0 232 711 1116 1143 1179
Size: 232 479 405 27 36
Fragments arranged by size:
479 405 232 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 631 1179
Size: 415 216 548
Fragments arranged by size:
548 415 216
BsiHKAI G_wGCw'C
Cuts at: 0 1022 1179
Size: 1022 157
BsmBI CGTCTCn'nnnn_
Cuts at: 0 336 1179
Size: 336 843
Bsp1286I G_dGCh'C
Cuts at: 0 896 1022 1179
Size: 896 126 157
Fragments arranged by size:
896 157 126
BspEI T'CCGG_A
Cuts at: 0 479 1179
Size: 479 700
BspHI T'CATG_A
Cuts at: 0 773 1179
Size: 773 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1080 1179
Size: 1080 99
BsrFI r'CCGG_y
Cuts at: 0 129 1179
Size: 129 1050
BstAPI GCAn_nnn'nTGC
Cuts at: 0 200 438 1179
Size: 200 238 741
Fragments arranged by size:
741 238 200
BstYI r'GATC_y
Cuts at: 0 314 1179
Size: 314 865
BtgI C'CryG_G
Cuts at: 0 259 270 527 1179
Size: 259 11 257 652
Fragments arranged by size:
652 259 257 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 175 1179
Size: 175 1004
BtsI GCAGTG_nn'
Cuts at: 0 1053 1179
Size: 1053 126
DraIII CAC_nnn'GTG
Cuts at: 0 384 438 1179
Size: 384 54 741
Fragments arranged by size:
741 384 54
EaeI y'GGCC_r
Cuts at: 0 348 635 1179
Size: 348 287 544
Fragments arranged by size:
544 348 287
EarI CTCTTCn'nnn_
Cuts at: 0 16 103 334 1033 1179
Size: 16 87 231 699 146
Fragments arranged by size:
699 231 146 87 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 358 994 1099 1120 1179
Size: 124 234 636 105 21 59
Fragments arranged by size:
636 234 124 105 59 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 1179
Size: 454 725
EcoO109I rG'GnC_Cy
Cuts at: 0 426 849 986 1152 1179
Size: 426 423 137 166 27
Fragments arranged by size:
426 423 166 137 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 827 847 859 879 1179
Size: 827 20 12 20 300
Fragments arranged by size:
827 300 20 20 12
MslI CAynn'nnrTG
Cuts at: 0 557 619 1179
Size: 557 62 560
Fragments arranged by size:
560 557 62
MspA1I CmG'CkG
Cuts at: 0 336 588 711 744 1179
Size: 336 252 123 33 435
Fragments arranged by size:
435 336 252 123 33
NcoI C'CATG_G
Cuts at: 0 259 270 1179
Size: 259 11 909
Fragments arranged by size:
909 259 11
NspI r_CATG'y
Cuts at: 0 421 624 720 1179
Size: 421 203 96 459
Fragments arranged by size:
459 421 203 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 852 1155 1179
Size: 384 468 303 24
Fragments arranged by size:
468 384 303 24
PfoI T'CCnGG_A
Cuts at: 0 851 1179
Size: 851 328
PpuMI rG'GwC_Cy
Cuts at: 0 849 1179
Size: 849 330
PshAI GACnn'nnGTC
Cuts at: 0 529 1179
Size: 529 650
PstI C_TGCA'G
Cuts at: 0 43 335 1091 1179
Size: 43 292 756 88
Fragments arranged by size:
756 292 88 43
PvuII CAG'CTG
Cuts at: 0 336 588 711 744 1179
Size: 336 252 123 33 435
Fragments arranged by size:
435 336 252 123 33
SapI GCTCTTCn'nnn_
Cuts at: 0 1033 1179
Size: 1033 146
SbfI CC_TGCA'GG
Cuts at: 0 1091 1179
Size: 1091 88
SfcI C'TryA_G
Cuts at: 0 39 331 791 1087 1179
Size: 39 292 460 296 92
Fragments arranged by size:
460 296 292 92 39
SmaI CCC'GGG
Cuts at: 0 217 699 1111 1179
Size: 217 482 412 68
Fragments arranged by size:
482 412 217 68
SmlI C'TyrA_G
Cuts at: 0 184 1179
Size: 184 995
SphI G_CATG'C
Cuts at: 0 421 1179
Size: 421 758
StuI AGG'CCT
Cuts at: 0 1012 1179
Size: 1012 167
StyI C'CwwG_G
Cuts at: 0 259 270 287 390 422 926 1179
Size: 259 11 17 103 32 504 253
Fragments arranged by size:
504 259 253 103 32 17 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 718 1179
Size: 643 75 461
Fragments arranged by size:
643 461 75
TatI w'GTAC_w
Cuts at: 0 240 1031 1179
Size: 240 791 148
Fragments arranged by size:
791 240 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 411 1179
Size: 379 32 768
Fragments arranged by size:
768 379 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 556 1179
Size: 556 623
XmaI C'CCGG_G
Cuts at: 0 215 697 1109 1179
Size: 215 482 412 70
Fragments arranged by size:
482 412 215 70
Enzymes that do cut:
AarI AcuI AhdI AlwNI AvaI BanII BbsI BglII
BlpI BmgBI BmrI BpmI BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrFI BstAPI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MslI MspA1I
NcoI NspI PflMI PfoI PpuMI PshAI PstI PvuII
SapI SbfI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI Bme1580I BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI