(Linear) (Six-base) MAPSORT of: KIEE5530.seq  Check: 895  from: 1  to: 1290



KIEE5530.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:04  ..

AccI GT'mk_AC

Cuts at:      0     265     852    1290 
   Size:        265     587     438

  Fragments arranged by size: 

                 587     438     265
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     621     992    1290 
   Size:         94     527     371     298

  Fragments arranged by size: 

                 527     371     298      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0      10    1290 
   Size:         10    1280
 
ApoI r'AATT_y

Cuts at:      0     223     388     476     724    1154    1290 
   Size:        223     165      88     248     430     136

  Fragments arranged by size: 

                 430     248     223     165     136      88
 
AseI AT'TA_AT

Cuts at:      0     340    1290 
   Size:        340     950
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      82     340     989    1290 
   Size:         82     258     649     301

  Fragments arranged by size: 

                 649     301     258      82
 
BclI T'GATC_A

Cuts at:      0     357    1290 
   Size:        357     933
 
BglII A'GATC_T

Cuts at:      0    1188    1290 
   Size:       1188     102
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     804    1290 
   Size:        804     486
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446    1290 
   Size:        446     844
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     833    1290 
   Size:        833     457
 
BseYI C'CCAG_C

Cuts at:      0     244    1290 
   Size:        244    1046
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1290 
   Size:        863     427
 
BsmI GAATG_Cn'

Cuts at:      0      90     705     954    1290 
   Size:         90     615     249     336

  Fragments arranged by size: 

                 615     336     249      90
 
BsrDI GCAATG_nn'

Cuts at:      0     204    1290 
   Size:        204    1086
 
BsrGI T'GTAC_A

Cuts at:      0    1080    1201    1290 
   Size:       1080     121      89

  Fragments arranged by size: 

                1080     121      89
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     669    1290 
   Size:        669     621
 
BstYI r'GATC_y

Cuts at:      0     657     870    1188    1290 
   Size:        657     213     318     102

  Fragments arranged by size: 

                 657     318     213     102
 
BstZ17I GTA'TAC

Cuts at:      0     266     853    1290 
   Size:        266     587     437

  Fragments arranged by size: 

                 587     437     266
 
DraI TTT'AAA

Cuts at:      0     429    1290 
   Size:        429     861
 
EarI CTCTTCn'nnn_

Cuts at:      0     370     640    1037    1290 
   Size:        370     270     397     253

  Fragments arranged by size: 

                 397     370     270     253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     621     992    1290 
   Size:         94     527     371     298

  Fragments arranged by size: 

                 527     371     298      94
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1010    1290 
   Size:       1010     280
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1015    1290 
   Size:       1015     275
 
EcoRI G'AATT_C

Cuts at:      0     476    1154    1290 
   Size:        476     678     136

  Fragments arranged by size: 

                 678     476     136
 
EcoRV GAT'ATC

Cuts at:      0     602    1290 
   Size:        602     688
 
HindIII A'AGCT_T

Cuts at:      0     347    1290 
   Size:        347     943
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1290 
   Size:        889     401
 
MslI CAynn'nnrTG

Cuts at:      0     752    1290 
   Size:        752     538
 
NspI r_CATG'y

Cuts at:      0     890    1079    1290 
   Size:        890     189     211

  Fragments arranged by size: 

                 890     211     189
 
PpuMI rG'GwC_Cy

Cuts at:      0    1015    1290 
   Size:       1015     275
 
PstI C_TGCA'G

Cuts at:      0     708     976    1290 
   Size:        708     268     314

  Fragments arranged by size: 

                 708     314     268
 
SfcI C'TryA_G

Cuts at:      0     704     941     972    1290 
   Size:        704     237      31     318

  Fragments arranged by size: 

                 704     318     237      31
 
SmlI C'TyrA_G

Cuts at:      0     461    1290 
   Size:        461     829
 
SspI AAT'ATT

Cuts at:      0    1170    1290 
   Size:       1170     120
 
StyI C'CwwG_G

Cuts at:      0     110     662    1036    1290 
   Size:        110     552     374     254

  Fragments arranged by size: 

                 552     374     254     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     916    1290 
   Size:        916     374
 
TatI w'GTAC_w

Cuts at:      0     996    1080    1201    1290 
   Size:        996      84     121      89

  Fragments arranged by size: 

                 996     121      89      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     701     733    1290 
   Size:        701      32     557

  Fragments arranged by size: 

                 701     557      32
 
XbaI T'CTAG_A

Cuts at:      0    1140    1290 
   Size:       1140     150
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1240    1290 
   Size:       1240      50
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     BbsI     BclI    BglII
     BplI    BpuEI    BseRI    BseYI     BsgI     BsmI    BsrDI    BsrGI
    BstXI    BstYI  BstZ17I     DraI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV  HindIII     MmeI     MslI     NspI    PpuMI     PstI
     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     XmnI     ZraI