Restriction map for
pFN21AE5522
(Linear) (Six-base) MAPSORT of: KIEE5522.seq Check: 9246 from: 1 to: 1071
KIEE5522.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AccI GT'mk_AC
Cuts at: 0 644 1071
Size: 644 427
AflIII A'CryG_T
Cuts at: 0 53 1071
Size: 53 1018
AgeI A'CCGG_T
Cuts at: 0 500 1071
Size: 500 571
AlwNI CAG_nnn'CTG
Cuts at: 0 579 979 1071
Size: 579 400 92
Fragments arranged by size:
579 400 92
ApaLI G'TGCA_C
Cuts at: 0 762 1071
Size: 762 309
ApoI r'AATT_y
Cuts at: 0 685 1071
Size: 685 386
AvaI C'yCGr_G
Cuts at: 0 114 196 827 1071
Size: 114 82 631 244
Fragments arranged by size:
631 244 114 82
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 559 592 1071
Size: 559 33 479
Fragments arranged by size:
559 479 33
BanI G'GyrC_C
Cuts at: 0 136 343 1033 1071
Size: 136 207 690 38
Fragments arranged by size:
690 207 136 38
BanII G_rGCy'C
Cuts at: 0 460 614 710 1071
Size: 460 154 96 361
Fragments arranged by size:
460 361 154 96
BbeI G_GCGC'C
Cuts at: 0 140 1071
Size: 140 931
BbsI GAAGACnn'nnnn_
Cuts at: 0 423 522 783 1071
Size: 423 99 261 288
Fragments arranged by size:
423 288 261 99
BglI GCCn_nnn'nGGC
Cuts at: 0 261 1071
Size: 261 810
BlpI GC'TnA_GC
Cuts at: 0 608 1071
Size: 608 463
Bme1580I G_kGCm'C
Cuts at: 0 766 1036 1071
Size: 766 270 35
Fragments arranged by size:
766 270 35
BmgBI CAC'GTC
Cuts at: 0 54 948 1071
Size: 54 894 123
Fragments arranged by size:
894 123 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 676 1071
Size: 145 531 395
Fragments arranged by size:
531 395 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 694 1071
Size: 376 318 377
Fragments arranged by size:
377 376 318
BsaI GGTCTCn'nnnn_
Cuts at: 0 872 1071
Size: 872 199
BsaHI Gr'CG_yC
Cuts at: 0 137 788 1071
Size: 137 651 283
Fragments arranged by size:
651 283 137
BsaWI w'CCGG_w
Cuts at: 0 500 1071
Size: 500 571
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 473 503 1071
Size: 473 30 568
Fragments arranged by size:
568 473 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 1071
Size: 481 590
BseYI C'CCAG_C
Cuts at: 0 321 783 1071
Size: 321 462 288
Fragments arranged by size:
462 321 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 730 1071
Size: 214 516 341
Fragments arranged by size:
516 341 214
BsiHKAI G_wGCw'C
Cuts at: 0 614 710 766 1071
Size: 614 96 56 305
Fragments arranged by size:
614 305 96 56
Bsp1286I G_dGCh'C
Cuts at: 0 460 614 710 766 1036 1071
Size: 460 154 96 56 270 35
Fragments arranged by size:
460 270 154 96 56 35
BspHI T'CATG_A
Cuts at: 0 614 1065 1071
Size: 614 451 6
Fragments arranged by size:
614 451 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 752 1071
Size: 752 319
BsrBI CCG'CTC
Cuts at: 0 201 923 1071
Size: 201 722 148
Fragments arranged by size:
722 201 148
BsrFI r'CCGG_y
Cuts at: 0 500 1071
Size: 500 571
BssSI C'ACGA_G
Cuts at: 0 895 1071
Size: 895 176
BstEII G'GTnAC_C
Cuts at: 0 84 1071
Size: 84 987
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1071
Size: 12 1059
BtgI C'CryG_G
Cuts at: 0 140 167 1041 1071
Size: 140 27 874 30
Fragments arranged by size:
874 140 30 27
BtsI GCAGTG_nn'
Cuts at: 0 764 1071
Size: 764 307
DraI TTT'AAA
Cuts at: 0 423 1071
Size: 423 648
EaeI y'GGCC_r
Cuts at: 0 506 845 934 1071
Size: 506 339 89 137
Fragments arranged by size:
506 339 137 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 242 1071
Size: 242 829
EcoICRI GAG'CTC
Cuts at: 0 612 708 1071
Size: 612 96 363
Fragments arranged by size:
612 363 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 676 1071
Size: 145 531 395
Fragments arranged by size:
531 395 145
EcoO109I rG'GnC_Cy
Cuts at: 0 732 1011 1071
Size: 732 279 60
Fragments arranged by size:
732 279 60
HaeII r_GCGC'y
Cuts at: 0 140 1071
Size: 140 931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1071
Size: 64 32 975
Fragments arranged by size:
975 64 32
HincII GTy'rAC
Cuts at: 0 645 1071
Size: 645 426
KasI G'GCGC_C
Cuts at: 0 136 1071
Size: 136 935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1026 1071
Size: 950 76 45
Fragments arranged by size:
950 76 45
MscI TGG'CCA
Cuts at: 0 508 936 1071
Size: 508 428 135
Fragments arranged by size:
508 428 135
MslI CAynn'nnrTG
Cuts at: 0 22 667 1071
Size: 22 645 404
Fragments arranged by size:
645 404 22
MspA1I CmG'CkG
Cuts at: 0 148 1043 1071
Size: 148 895 28
Fragments arranged by size:
895 148 28
NarI GG'CG_CC
Cuts at: 0 137 1071
Size: 137 934
NcoI C'CATG_G
Cuts at: 0 167 1071
Size: 167 904
NspI r_CATG'y
Cuts at: 0 195 214 1071
Size: 195 19 857
Fragments arranged by size:
857 195 19
PfoI T'CCnGG_A
Cuts at: 0 122 692 1071
Size: 122 570 379
Fragments arranged by size:
570 379 122
PpuMI rG'GwC_Cy
Cuts at: 0 732 1071
Size: 732 339
PspXI vC'TCGA_Gb
Cuts at: 0 196 1071
Size: 196 875
PstI C_TGCA'G
Cuts at: 0 158 575 1071
Size: 158 417 496
Fragments arranged by size:
496 417 158
SacI G_AGCT'C
Cuts at: 0 614 710 1071
Size: 614 96 361
Fragments arranged by size:
614 361 96
SacII CC_GC'GG
Cuts at: 0 1044 1071
Size: 1044 27
SalI G'TCGA_C
Cuts at: 0 643 1071
Size: 643 428
SfcI C'TryA_G
Cuts at: 0 154 571 1071
Size: 154 417 500
Fragments arranged by size:
500 417 154
SfoI GGC'GCC
Cuts at: 0 138 1071
Size: 138 933
SmaI CCC'GGG
Cuts at: 0 829 1071
Size: 829 242
SmlI C'TyrA_G
Cuts at: 0 196 391 709 1071
Size: 196 195 318 362
Fragments arranged by size:
362 318 196 195
SphI G_CATG'C
Cuts at: 0 214 1071
Size: 214 857
SspI AAT'ATT
Cuts at: 0 474 1071
Size: 474 597
StuI AGG'CCT
Cuts at: 0 304 1071
Size: 304 767
StyI C'CwwG_G
Cuts at: 0 167 633 1071
Size: 167 466 438
Fragments arranged by size:
466 438 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 1071
Size: 103 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 991 1071
Size: 354 637 80
Fragments arranged by size:
637 354 80
XhoI C'TCGA_G
Cuts at: 0 196 1071
Size: 196 875
XmaI C'CCGG_G
Cuts at: 0 827 1071
Size: 827 244
Enzymes that do cut:
AccI AflIII AgeI AlwNI ApaLI ApoI AvaI BaeI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstEII BstXI BtgI BtsI DraI EaeI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PfoI PpuMI PspXI
PstI SacI SacII SalI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI
BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
PvuII RsrII SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SwaI TatI TstI
Tth111I XbaI XmnI ZraI