Restriction map for
pFN21AE5521
(Linear) (Six-base) MAPSORT of: KIEE5521.seq Check: 7515 from: 1 to: 1311
KIEE5521.orf
With 182 enzymes: SgfI *
January 29, 2014 12:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1301 1311
Size: 1301 10
AccI GT'mk_AC
Cuts at: 0 1082 1311
Size: 1082 229
AclI AA'CG_TT
Cuts at: 0 1120 1311
Size: 1120 191
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 319 1057 1311
Size: 187 132 738 254
Fragments arranged by size:
738 254 187 132
AflII C'TTAA_G
Cuts at: 0 187 1311
Size: 187 1124
AflIII A'CryG_T
Cuts at: 0 482 1311
Size: 482 829
AleI CACnn'nnGTG
Cuts at: 0 860 1311
Size: 860 451
AlwNI CAG_nnn'CTG
Cuts at: 0 244 895 1311
Size: 244 651 416
Fragments arranged by size:
651 416 244
ApoI r'AATT_y
Cuts at: 0 431 1311
Size: 431 880
AvaI C'yCGr_G
Cuts at: 0 10 272 1311
Size: 10 262 1039
Fragments arranged by size:
1039 262 10
BanII G_rGCy'C
Cuts at: 0 279 1135 1311
Size: 279 856 176
Fragments arranged by size:
856 279 176
BciVI GTATCCnnnnn_n'
Cuts at: 0 718 1004 1311
Size: 718 286 307
Fragments arranged by size:
718 307 286
BfrBI ATG'CAT
Cuts at: 0 447 973 1180 1311
Size: 447 526 207 131
Fragments arranged by size:
526 447 207 131
BglII A'GATC_T
Cuts at: 0 330 1311
Size: 330 981
Bme1580I G_kGCm'C
Cuts at: 0 86 1311
Size: 86 1225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1311
Size: 367 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1311
Size: 765 546
BsaAI yAC'GTr
Cuts at: 0 1198 1311
Size: 1198 113
BsaBI GATnn'nnATC
Cuts at: 0 1237 1311
Size: 1237 74
BseYI C'CCAG_C
Cuts at: 0 208 534 660 1311
Size: 208 326 126 651
Fragments arranged by size:
651 326 208 126
BsiHKAI G_wGCw'C
Cuts at: 0 1135 1311
Size: 1135 176
BsmI GAATG_Cn'
Cuts at: 0 1071 1311
Size: 1071 240
Bsp1286I G_dGCh'C
Cuts at: 0 86 279 1135 1311
Size: 86 193 856 176
Fragments arranged by size:
856 193 176 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1301 1311
Size: 1301 10
BsrGI T'GTAC_A
Cuts at: 0 1252 1311
Size: 1252 59
BstBI TT'CG_AA
Cuts at: 0 930 1190 1311
Size: 930 260 121
Fragments arranged by size:
930 260 121
BstYI r'GATC_y
Cuts at: 0 330 385 1311
Size: 330 55 926
Fragments arranged by size:
926 330 55
BstZ17I GTA'TAC
Cuts at: 0 1083 1311
Size: 1083 228
BtgI C'CryG_G
Cuts at: 0 795 1311
Size: 795 516
ClaI AT'CG_AT
Cuts at: 0 750 1311
Size: 750 561
DraI TTT'AAA
Cuts at: 0 530 1311
Size: 530 781
EarI CTCTTCn'nnn_
Cuts at: 0 143 644 1311
Size: 143 501 667
Fragments arranged by size:
667 501 143
EcoICRI GAG'CTC
Cuts at: 0 1133 1311
Size: 1133 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 319 367 1057 1311
Size: 187 132 48 690 254
Fragments arranged by size:
690 254 187 132 48
EcoO109I rG'GnC_Cy
Cuts at: 0 318 1311
Size: 318 993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 655 1311
Size: 655 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 536 568 732 764 1208 1240
Size: 435 32 69 32 164 32 444 32
Cuts at: 1240 1311
Size: 71
Fragments arranged by size:
444 435 164 71 69 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1311
Size: 718 593
MslI CAynn'nnrTG
Cuts at: 0 481 706 860 974 1311
Size: 481 225 154 114 337
Fragments arranged by size:
481 337 225 154 114
MspA1I CmG'CkG
Cuts at: 0 34 1311
Size: 34 1277
NdeI CA'TA_TG
Cuts at: 0 159 504 975 1311
Size: 159 345 471 336
Fragments arranged by size:
471 345 336 159
NsiI A_TGCA'T
Cuts at: 0 449 975 1182 1311
Size: 449 526 207 129
Fragments arranged by size:
526 449 207 129
NspI r_CATG'y
Cuts at: 0 486 1311
Size: 486 825
PciI A'CATG_T
Cuts at: 0 482 1311
Size: 482 829
PpuMI rG'GwC_Cy
Cuts at: 0 318 1311
Size: 318 993
PsiI TTA'TAA
Cuts at: 0 98 1055 1311
Size: 98 957 256
Fragments arranged by size:
957 256 98
PstI C_TGCA'G
Cuts at: 0 1050 1311
Size: 1050 261
PvuII CAG'CTG
Cuts at: 0 34 1311
Size: 34 1277
SacI G_AGCT'C
Cuts at: 0 1135 1311
Size: 1135 176
SapI GCTCTTCn'nnn_
Cuts at: 0 644 1311
Size: 644 667
SfcI C'TryA_G
Cuts at: 0 914 1046 1311
Size: 914 132 265
Fragments arranged by size:
914 265 132
SmlI C'TyrA_G
Cuts at: 0 187 780 1311
Size: 187 593 531
Fragments arranged by size:
593 531 187
StuI AGG'CCT
Cuts at: 0 473 1311
Size: 473 838
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 1311
Size: 385 926
TatI w'GTAC_w
Cuts at: 0 311 1252 1311
Size: 311 941 59
Fragments arranged by size:
941 311 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 561 593 1311
Size: 561 32 718
Fragments arranged by size:
718 561 32
XmnI GAAnn'nnTTC
Cuts at: 0 332 431 638 1311
Size: 332 99 207 673
Fragments arranged by size:
673 332 207 99
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AlwNI
ApoI AvaI BanII BciVI BfrBI BglII Bme1580I BpmI
BpuEI BsaAI BsaBI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BstBI BstYI BstZ17I BtgI ClaI DraI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I MmeI MslI MspA1I
NdeI NsiI NspI PciI PpuMI PsiI PstI PvuII
SacI SapI SfcI SmlI StuI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BclI BglI BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI Bsu36I
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI