(Linear) (Six-base) MAPSORT of: KIEE5519.seq  Check: 1251  from: 1  to: 1395



KIEE5519.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:04  ..

AccI GT'mk_AC

Cuts at:      0     532    1395 
   Size:        532     863
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     553    1395 
   Size:        432     121     842

  Fragments arranged by size: 

                 842     432     121
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1139    1395 
   Size:       1139     256
 
AlwNI CAG_nnn'CTG

Cuts at:      0     898    1395 
   Size:        898     497
 
ApoI r'AATT_y

Cuts at:      0     173     197     204     373     425     506     613    1395 
   Size:        173      24       7     169      52      81     107     782

  Fragments arranged by size: 

                 782     173     169     107      81      52      24       7
            
 
AseI AT'TA_AT

Cuts at:      0     314     551    1395 
   Size:        314     237     844

  Fragments arranged by size: 

                 844     314     237
 
BamHI G'GATC_C

Cuts at:      0    1153    1395 
   Size:       1153     242
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      63    1265    1395 
   Size:         63    1202     130

  Fragments arranged by size: 

                1202     130      63
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     890     924    1395 
   Size:        890      34     471

  Fragments arranged by size: 

                 890     471      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     622    1286    1395 
   Size:        622     664     109

  Fragments arranged by size: 

                 664     622     109
 
BfrBI ATG'CAT

Cuts at:      0     842    1395 
   Size:        842     553
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     717    1395 
   Size:        717     678
 
Bpu10I CC'TnA_GC

Cuts at:      0     796    1395 
   Size:        796     599
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441    1395 
   Size:        441     954
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1147    1395 
   Size:       1147     248
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      36      66    1395 
   Size:         36      30    1329

  Fragments arranged by size: 

                1329      36      30
 
BsmI GAATG_Cn'

Cuts at:      0     842    1395 
   Size:        842     553
 
BspHI T'CATG_A

Cuts at:      0     177     776    1395 
   Size:        177     599     619

  Fragments arranged by size: 

                 619     599     177
 
BsrDI GCAATG_nn'

Cuts at:      0     208    1304    1395 
   Size:        208    1096      91

  Fragments arranged by size: 

                1096     208      91
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     898    1395 
   Size:        898     497
 
BstBI TT'CG_AA

Cuts at:      0     504    1395 
   Size:        504     891
 
BstYI r'GATC_y

Cuts at:      0     999    1153    1273    1395 
   Size:        999     154     120     122

  Fragments arranged by size: 

                 999     154     122     120
 
BstZ17I GTA'TAC

Cuts at:      0     533    1395 
   Size:        533     862
 
Bsu36I CC'TnA_GG

Cuts at:      0     974    1395 
   Size:        974     421
 
BtgI C'CryG_G

Cuts at:      0     587    1395 
   Size:        587     808
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1009    1395 
   Size:       1009     386
 
BtsI GCAGTG_nn'

Cuts at:      0     899    1395 
   Size:        899     496
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     249     284    1395 
   Size:        249      35    1111

  Fragments arranged by size: 

                1111     249      35
 
DraI TTT'AAA

Cuts at:      0     371    1395 
   Size:        371    1024
 
EarI CTCTTCn'nnn_

Cuts at:      0      28    1395 
   Size:         28    1367
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     553     717    1395 
   Size:        432     121     164     678

  Fragments arranged by size: 

                 678     432     164     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4    1395 
   Size:          4    1391
 
EcoRI G'AATT_C

Cuts at:      0     204    1395 
   Size:        204    1191
 
EcoRV GAT'ATC

Cuts at:      0     189    1395 
   Size:        189    1206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     471     503     690     722    1125    1157    1395 
   Size:        471      32     187      32     403      32     238

  Fragments arranged by size: 

                 471     403     238     187      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      14    1395 
   Size:         14    1381
 
MfeI C'AATT_G

Cuts at:      0     953    1395 
   Size:        953     442
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     399    1395 
   Size:        399     996
 
MslI CAynn'nnrTG

Cuts at:      0     391    1395 
   Size:        391    1004
 
NcoI C'CATG_G

Cuts at:      0     587    1395 
   Size:        587     808
 
NsiI A_TGCA'T

Cuts at:      0     844    1395 
   Size:        844     551
 
PfoI T'CCnGG_A

Cuts at:      0    1149    1395 
   Size:       1149     246
 
PpuMI rG'GwC_Cy

Cuts at:      0       4    1395 
   Size:          4    1391
 
PstI C_TGCA'G

Cuts at:      0    1267    1395 
   Size:       1267     128
 
SexAI A'CCwGG_T

Cuts at:      0     352    1395 
   Size:        352    1043
 
SfcI C'TryA_G

Cuts at:      0     923    1263    1395 
   Size:        923     340     132

  Fragments arranged by size: 

                 923     340     132
 
SmlI C'TyrA_G

Cuts at:      0     456    1395 
   Size:        456     939
 
SspI AAT'ATT

Cuts at:      0    1179    1334    1395 
   Size:       1179     155      61

  Fragments arranged by size: 

                1179     155      61
 
StyI C'CwwG_G

Cuts at:      0     587    1395 
   Size:        587     808
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     855    1395 
   Size:        855     540
 
TatI w'GTAC_w

Cuts at:      0     949    1395 
   Size:        949     446
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1149    1181    1395 
   Size:       1149      32     214

  Fragments arranged by size: 

                1149     214      32
 
XbaI T'CTAG_A

Cuts at:      0    1327    1395 
   Size:       1327      68
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI     ApoI     AseI    BamHI     BbsI
     BcgI    BciVI    BfrBI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI
     BsmI    BspHI    BsrDI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV    Hin4I  HindIII     MfeI     MmeI     MslI     NcoI
     NsiI     PfoI    PpuMI     PstI    SexAI     SfcI     SmlI     SspI
     StyI    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI