Restriction map for
pFN21AE5511
(Linear) (Six-base) MAPSORT of: KIEE5511.seq Check: 5817 from: 1 to: 1224
KIEE5511.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 547 1224
Size: 162 385 677
Fragments arranged by size:
677 385 162
AfeI AGC'GCT
Cuts at: 0 154 1224
Size: 154 1070
ApaI G_GGCC'C
Cuts at: 0 28 510 1224
Size: 28 482 714
Fragments arranged by size:
714 482 28
ApoI r'AATT_y
Cuts at: 0 1156 1224
Size: 1156 68
AvaI C'yCGr_G
Cuts at: 0 27 245 1145 1224
Size: 27 218 900 79
Fragments arranged by size:
900 218 79 27
BamHI G'GATC_C
Cuts at: 0 866 1224
Size: 866 358
BanI G'GyrC_C
Cuts at: 0 100 295 313 1016 1224
Size: 100 195 18 703 208
Fragments arranged by size:
703 208 195 100 18
BanII G_rGCy'C
Cuts at: 0 28 510 1224
Size: 28 482 714
Fragments arranged by size:
714 482 28
BbeI G_GCGC'C
Cuts at: 0 104 299 1020 1224
Size: 104 195 721 204
Fragments arranged by size:
721 204 195 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 780 1224
Size: 102 678 444
Fragments arranged by size:
678 444 102
BbvCI CC'TCA_GC
Cuts at: 0 636 1224
Size: 636 588
BclI T'GATC_A
Cuts at: 0 446 1224
Size: 446 778
BglI GCCn_nnn'nGGC
Cuts at: 0 247 563 1069 1078 1224
Size: 247 316 506 9 146
Fragments arranged by size:
506 316 247 146 9
Bme1580I G_kGCm'C
Cuts at: 0 28 316 510 1224
Size: 28 288 194 714
Fragments arranged by size:
714 288 194 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1224
Size: 1056 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 457 684 1224
Size: 142 315 227 540
Fragments arranged by size:
540 315 227 142
Bpu10I CC'TnA_GC
Cuts at: 0 196 636 1224
Size: 196 440 588
Fragments arranged by size:
588 440 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 1201 1224
Size: 1201 23
BsaBI GATnn'nnATC
Cuts at: 0 451 1224
Size: 451 773
BsaHI Gr'CG_yC
Cuts at: 0 101 296 1017 1224
Size: 101 195 721 207
Fragments arranged by size:
721 207 195 101
BsaWI w'CCGG_w
Cuts at: 0 494 956 1224
Size: 494 462 268
Fragments arranged by size:
494 462 268
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 250 1224
Size: 175 75 974
Fragments arranged by size:
974 175 75
BseYI C'CCAG_C
Cuts at: 0 20 93 155 281 1224
Size: 20 73 62 126 943
Fragments arranged by size:
943 126 73 62 20
BsiHKAI G_wGCw'C
Cuts at: 0 86 1224
Size: 86 1138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 492 1224
Size: 492 732
Bsp1286I G_dGCh'C
Cuts at: 0 28 86 316 510 1224
Size: 28 58 230 194 714
Fragments arranged by size:
714 230 194 58 28
BspEI T'CCGG_A
Cuts at: 0 494 956 1224
Size: 494 462 268
Fragments arranged by size:
494 462 268
BspHI T'CATG_A
Cuts at: 0 467 1187 1224
Size: 467 720 37
Fragments arranged by size:
720 467 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 1224
Size: 98 1126
BsrDI GCAATG_nn'
Cuts at: 0 907 1224
Size: 907 317
BsrFI r'CCGG_y
Cuts at: 0 250 292 562 1019 1224
Size: 250 42 270 457 205
Fragments arranged by size:
457 270 250 205 42
BsrGI T'GTAC_A
Cuts at: 0 644 1224
Size: 644 580
BssHII G'CGCG_C
Cuts at: 0 52 1224
Size: 52 1172
BssSI C'ACGA_G
Cuts at: 0 133 1224
Size: 133 1091
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1140 1224
Size: 1140 84
BstYI r'GATC_y
Cuts at: 0 866 1224
Size: 866 358
Bsu36I CC'TnA_GG
Cuts at: 0 871 1224
Size: 871 353
BtgI C'CryG_G
Cuts at: 0 183 1224
Size: 183 1041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 441 1224
Size: 441 783
BtsI GCAGTG_nn'
Cuts at: 0 547 1224
Size: 547 677
EaeI y'GGCC_r
Cuts at: 0 6 68 170 186 560 1079 1109 1224
Size: 6 62 102 16 374 519 30 115
Fragments arranged by size:
519 374 115 102 62 30 16 6
EarI CTCTTCn'nnn_
Cuts at: 0 436 1224
Size: 436 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 262 1224
Size: 214 48 962
Fragments arranged by size:
962 214 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 162 457 547 684 1224
Size: 142 20 295 90 137 540
Fragments arranged by size:
540 295 142 137 90 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 514 1224
Size: 40 474 710
Fragments arranged by size:
710 474 40
EcoO109I rG'GnC_Cy
Cuts at: 0 24 506 507 723 1224
Size: 24 482 1 216 501
Fragments arranged by size:
501 482 216 24 1
EcoRI G'AATT_C
Cuts at: 0 1156 1224
Size: 1156 68
HaeII r_GCGC'y
Cuts at: 0 104 141 156 168 299 444 1020 1224
Size: 104 37 15 12 131 145 576 204
Fragments arranged by size:
576 204 145 131 104 37 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 333 356 365 388 1224
Size: 333 23 9 23 836
Fragments arranged by size:
836 333 23 23 9
KasI G'GCGC_C
Cuts at: 0 100 295 1016 1224
Size: 100 195 721 208
Fragments arranged by size:
721 208 195 100
MscI TGG'CCA
Cuts at: 0 172 1081 1111 1224
Size: 172 909 30 113
Fragments arranged by size:
909 172 113 30
MslI CAynn'nnrTG
Cuts at: 0 259 796 1224
Size: 259 537 428
Fragments arranged by size:
537 428 259
MspA1I CmG'CkG
Cuts at: 0 20 185 486 887 965 1224
Size: 20 165 301 401 78 259
Fragments arranged by size:
401 301 259 165 78 20
NaeI GCC'GGC
Cuts at: 0 252 564 1021 1224
Size: 252 312 457 203
Fragments arranged by size:
457 312 252 203
NarI GG'CG_CC
Cuts at: 0 101 296 1017 1224
Size: 101 195 721 207
Fragments arranged by size:
721 207 195 101
NgoMIV G'CCGG_C
Cuts at: 0 250 562 1019 1224
Size: 250 312 457 205
Fragments arranged by size:
457 312 250 205
PfoI T'CCnGG_A
Cuts at: 0 434 1224
Size: 434 790
PpuMI rG'GwC_Cy
Cuts at: 0 723 1224
Size: 723 501
PspOMI G'GGCC_C
Cuts at: 0 24 506 1224
Size: 24 482 718
Fragments arranged by size:
718 482 24
PstI C_TGCA'G
Cuts at: 0 1091 1224
Size: 1091 133
PvuII CAG'CTG
Cuts at: 0 20 1224
Size: 20 1204
SacII CC_GC'GG
Cuts at: 0 186 1224
Size: 186 1038
SexAI A'CCwGG_T
Cuts at: 0 725 1224
Size: 725 499
SfcI C'TryA_G
Cuts at: 0 1087 1224
Size: 1087 137
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 247 1078 1224
Size: 247 831 146
Fragments arranged by size:
831 247 146
SfoI GGC'GCC
Cuts at: 0 102 297 1018 1224
Size: 102 195 721 206
Fragments arranged by size:
721 206 195 102
SgrAI Cr'CCGG_yG
Cuts at: 0 292 1224
Size: 292 932
SmaI CCC'GGG
Cuts at: 0 29 1147 1224
Size: 29 1118 77
Fragments arranged by size:
1118 77 29
SrfI GCCC'GGGC
Cuts at: 0 29 1224
Size: 29 1195
StuI AGG'CCT
Cuts at: 0 764 1072 1224
Size: 764 308 152
Fragments arranged by size:
764 308 152
StyI C'CwwG_G
Cuts at: 0 759 1224
Size: 759 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 790 1224
Size: 389 401 434
Fragments arranged by size:
434 401 389
TatI w'GTAC_w
Cuts at: 0 581 644 1224
Size: 581 63 580
Fragments arranged by size:
581 580 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 874 906 1224
Size: 874 32 318
Fragments arranged by size:
874 318 32
Tth111I GACn'n_nGTC
Cuts at: 0 268 727 1224
Size: 268 459 497
Fragments arranged by size:
497 459 268
XmaI C'CCGG_G
Cuts at: 0 27 1145 1224
Size: 27 1118 79
Fragments arranged by size:
1118 79 27
Enzymes that do cut:
AcuI AfeI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI Bme1580I BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV
PfoI PpuMI PspOMI PstI PvuII SacII SexAI SfcI
SfiI SfoI SgrAI SmaI SrfI StuI StyI TaqII
TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaAI BsaXI BsgI BsiEI
BsiWI BsmI BsrBI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SgfI SmlI SnaBI SpeI
SphI SspI SwaI XbaI XcmI XhoI XmnI ZraI