Restriction map for
pFN21AE5509
(Linear) (Six-base) MAPSORT of: KIEE5509.seq Check: 7096 from: 1 to: 1290
KIEE5509.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AatII G_ACGT'C
Cuts at: 0 1211 1290
Size: 1211 79
AclI AA'CG_TT
Cuts at: 0 680 1290
Size: 680 610
AfeI AGC'GCT
Cuts at: 0 1255 1290
Size: 1255 35
AflIII A'CryG_T
Cuts at: 0 992 1290
Size: 992 298
AhdI GACnn_n'nnGTC
Cuts at: 0 56 433 1290
Size: 56 377 857
Fragments arranged by size:
857 377 56
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 485 1290
Size: 485 805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 63 95 1290
Size: 63 32 1195
Fragments arranged by size:
1195 63 32
AlwNI CAG_nnn'CTG
Cuts at: 0 316 1290
Size: 316 974
ApaI G_GGCC'C
Cuts at: 0 1169 1290
Size: 1169 121
ApaLI G'TGCA_C
Cuts at: 0 194 1290
Size: 194 1096
AseI AT'TA_AT
Cuts at: 0 1019 1290
Size: 1019 271
AvaI C'yCGr_G
Cuts at: 0 1160 1290
Size: 1160 130
BamHI G'GATC_C
Cuts at: 0 1179 1290
Size: 1179 111
BanII G_rGCy'C
Cuts at: 0 1058 1169 1290
Size: 1058 111 121
Fragments arranged by size:
1058 121 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1240 1290
Size: 863 377 50
Fragments arranged by size:
863 377 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 1290
Size: 28 1262
BciVI GTATCCnnnnn_n'
Cuts at: 0 656 1290
Size: 656 634
BlpI GC'TnA_GC
Cuts at: 0 1027 1290
Size: 1027 263
Bme1580I G_kGCm'C
Cuts at: 0 198 1169 1290
Size: 198 971 121
Fragments arranged by size:
971 198 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 163 221 1230 1290
Size: 163 58 1009 60
Fragments arranged by size:
1009 163 60 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1044 1290
Size: 344 700 246
Fragments arranged by size:
700 344 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 688 1191 1290
Size: 207 481 503 99
Fragments arranged by size:
503 481 207 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 1011 1290
Size: 1011 279
BsaHI Gr'CG_yC
Cuts at: 0 1208 1290
Size: 1208 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 63 93 1290
Size: 63 30 1197
Fragments arranged by size:
1197 63 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1223 1290
Size: 481 742 67
Fragments arranged by size:
742 481 67
BsiEI CG_ry'CG
Cuts at: 0 8 1290
Size: 8 1282
BsiHKAI G_wGCw'C
Cuts at: 0 198 1290
Size: 198 1092
Bsp1286I G_dGCh'C
Cuts at: 0 198 1058 1169 1290
Size: 198 860 111 121
Fragments arranged by size:
860 198 121 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 46 751 1290
Size: 46 705 539
Fragments arranged by size:
705 539 46
BsrDI GCAATG_nn'
Cuts at: 0 141 467 1290
Size: 141 326 823
Fragments arranged by size:
823 326 141
BsrFI r'CCGG_y
Cuts at: 0 971 1290
Size: 971 319
BssHII G'CGCG_C
Cuts at: 0 15 1290
Size: 15 1275
BstYI r'GATC_y
Cuts at: 0 372 1179 1290
Size: 372 807 111
Fragments arranged by size:
807 372 111
BtgI C'CryG_G
Cuts at: 0 8 1050 1290
Size: 8 1042 240
Fragments arranged by size:
1042 240 8
BtsI GCAGTG_nn'
Cuts at: 0 82 196 464 1290
Size: 82 114 268 826
Fragments arranged by size:
826 268 114 82
ClaI AT'CG_AT
Cuts at: 0 718 1290
Size: 718 572
DraI TTT'AAA
Cuts at: 0 894 1290
Size: 894 396
EaeI y'GGCC_r
Cuts at: 0 138 1290
Size: 138 1152
EarI CTCTTCn'nnn_
Cuts at: 0 115 333 482 524 1051 1270 1290
Size: 115 218 149 42 527 219 20
Fragments arranged by size:
527 219 218 149 115 42 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1044 1290
Size: 344 700 246
Fragments arranged by size:
700 344 246
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 1290
Size: 1036 254
EcoO109I rG'GnC_Cy
Cuts at: 0 1041 1098 1165 1166 1290
Size: 1041 57 67 1 124
Fragments arranged by size:
1041 124 67 57 1
HaeII r_GCGC'y
Cuts at: 0 305 1257 1290
Size: 305 952 33
Fragments arranged by size:
952 305 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 419 451 1164 1196 1290
Size: 42 32 345 32 713 32 94
Fragments arranged by size:
713 345 94 42 32 32 32
MscI TGG'CCA
Cuts at: 0 140 1290
Size: 140 1150
MslI CAynn'nnrTG
Cuts at: 0 1021 1290
Size: 1021 269
MspA1I CmG'CkG
Cuts at: 0 10 944 1083 1290
Size: 10 934 139 207
Fragments arranged by size:
934 207 139 10
NaeI GCC'GGC
Cuts at: 0 973 1290
Size: 973 317
NdeI CA'TA_TG
Cuts at: 0 642 1290
Size: 642 648
NgoMIV G'CCGG_C
Cuts at: 0 971 1290
Size: 971 319
NspI r_CATG'y
Cuts at: 0 996 1290
Size: 996 294
PasI CC'CwG_GG
Cuts at: 0 1038 1290
Size: 1038 252
PciI A'CATG_T
Cuts at: 0 992 1290
Size: 992 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 1110 1290
Size: 1110 180
PfoI T'CCnGG_A
Cuts at: 0 1118 1290
Size: 1118 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 1290
Size: 63 32 1195
Fragments arranged by size:
1195 63 32
PshAI GACnn'nnGTC
Cuts at: 0 623 1290
Size: 623 667
PspOMI G'GGCC_C
Cuts at: 0 1165 1290
Size: 1165 125
PstI C_TGCA'G
Cuts at: 0 203 285 762 1290
Size: 203 82 477 528
Fragments arranged by size:
528 477 203 82
PvuII CAG'CTG
Cuts at: 0 1083 1290
Size: 1083 207
SacII CC_GC'GG
Cuts at: 0 11 1290
Size: 11 1279
SapI GCTCTTCn'nnn_
Cuts at: 0 482 1290
Size: 482 808
SfcI C'TryA_G
Cuts at: 0 199 281 758 1290
Size: 199 82 477 532
Fragments arranged by size:
532 477 199 82
SmaI CCC'GGG
Cuts at: 0 1162 1290
Size: 1162 128
SmlI C'TyrA_G
Cuts at: 0 186 667 1170 1290
Size: 186 481 503 120
Fragments arranged by size:
503 481 186 120
SspI AAT'ATT
Cuts at: 0 869 1290
Size: 869 421
StyI C'CwwG_G
Cuts at: 0 1001 1290
Size: 1001 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 554 1051 1290
Size: 184 370 497 239
Fragments arranged by size:
497 370 239 184
XmaI C'CCGG_G
Cuts at: 0 1160 1290
Size: 1160 130
ZraI GAC'GTC
Cuts at: 0 1209 1290
Size: 1209 81
Enzymes that do cut:
AatII AclI AfeI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI AseI AvaI BamHI BanII BbsI BcgI
BciVI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI
BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssHII BstYI BtgI BtsI ClaI DraI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I
NaeI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PshAI PspOMI PstI PvuII SacII SapI SfcI
SmaI SmlI SspI StyI TaqII XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AcuI AflII AgeI AleI ApoI
AscI AsiSI AvrII BaeI BanI BbeI BbvCI BclI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NheI NotI NruI NsiI PacI PmeI PmlI
PpuMI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI