(Linear) (Six-base) MAPSORT of: KIEE5507.seq  Check: 8318  from: 1  to: 1242



KIEE5507.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AccI GT'mk_AC

Cuts at:      0    1181    1242 
   Size:       1181      61
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     824    1242 
   Size:        227     597     418

  Fragments arranged by size: 

                 597     418     227
 
AflIII A'CryG_T

Cuts at:      0     771    1242 
   Size:        771     471
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1050    1242 
   Size:       1050     192
 
AlwNI CAG_nnn'CTG

Cuts at:      0     151     154    1242 
   Size:        151       3    1088

  Fragments arranged by size: 

                1088     151       3
 
ApaLI G'TGCA_C

Cuts at:      0    1035    1242 
   Size:       1035     207
 
ApoI r'AATT_y

Cuts at:      0      61     484    1013    1242 
   Size:         61     423     529     229

  Fragments arranged by size: 

                 529     423     229      61
 
BanII G_rGCy'C

Cuts at:      0    1008    1242 
   Size:       1008     234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     624     830    1242 
   Size:        624     206     412

  Fragments arranged by size: 

                 624     412     206
 
Bme1580I G_kGCm'C

Cuts at:      0    1039    1242 
   Size:       1039     203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     533    1242 
   Size:        224     309     709

  Fragments arranged by size: 

                 709     309     224
 
BsaAI yAC'GTr

Cuts at:      0    1114    1242 
   Size:       1114     128
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1043    1242 
   Size:       1043     199
 
BsiHKAI G_wGCw'C

Cuts at:      0     649    1008    1039    1242 
   Size:        649     359      31     203

  Fragments arranged by size: 

                 649     359     203      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     649    1008    1039    1242 
   Size:        649     359      31     203

  Fragments arranged by size: 

                 649     359     203      31
 
BsrDI GCAATG_nn'

Cuts at:      0     851    1242 
   Size:        851     391
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     853    1242 
   Size:        853     389
 
DraI TTT'AAA

Cuts at:      0      36     218    1242 
   Size:         36     182    1024

  Fragments arranged by size: 

                1024     182      36
 
DraIII CAC_nnn'GTG

Cuts at:      0     592    1242 
   Size:        592     650
 
EaeI y'GGCC_r

Cuts at:      0     683    1242 
   Size:        683     559
 
EcoICRI GAG'CTC

Cuts at:      0    1006    1242 
   Size:       1006     236
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     227     533     824    1242 
   Size:        224       3     306     291     418

  Fragments arranged by size: 

                 418     306     291     224       3
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     310     641    1242 
   Size:        310     331     601

  Fragments arranged by size: 

                 601     331     310
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     222     254    1242 
   Size:        222      32     988

  Fragments arranged by size: 

                 988     222      32
 
HincII GTy'rAC

Cuts at:      0     470    1242 
   Size:        470     772
 
HindIII A'AGCT_T

Cuts at:      0    1205    1242 
   Size:       1205      37
 
MfeI C'AATT_G

Cuts at:      0     332    1242 
   Size:        332     910
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1179    1242 
   Size:       1179      63
 
MscI TGG'CCA

Cuts at:      0     685    1242 
   Size:        685     557
 
MspA1I CmG'CkG

Cuts at:      0     151     399    1242 
   Size:        151     248     843

  Fragments arranged by size: 

                 843     248     151
 
NdeI CA'TA_TG

Cuts at:      0     440    1242 
   Size:        440     802
 
NspI r_CATG'y

Cuts at:      0     600     884    1242 
   Size:        600     284     358

  Fragments arranged by size: 

                 600     358     284
 
PfoI T'CCnGG_A

Cuts at:      0      87    1242 
   Size:         87    1155
 
PstI C_TGCA'G

Cuts at:      0      24    1242 
   Size:         24    1218
 
PvuII CAG'CTG

Cuts at:      0     151     399    1242 
   Size:        151     248     843

  Fragments arranged by size: 

                 843     248     151
 
SacI G_AGCT'C

Cuts at:      0    1008    1242 
   Size:       1008     234
 
ScaI AGT'ACT

Cuts at:      0     959    1242 
   Size:        959     283
 
SexAI A'CCwGG_T

Cuts at:      0     588    1242 
   Size:        588     654
 
SfcI C'TryA_G

Cuts at:      0      20    1242 
   Size:         20    1222
 
SpeI A'CTAG_T

Cuts at:      0     130    1242 
   Size:        130    1112
 
SspI AAT'ATT

Cuts at:      0     270    1242 
   Size:        270     972
 
TatI w'GTAC_w

Cuts at:      0     282     864     957    1242 
   Size:        282     582      93     285

  Fragments arranged by size: 

                 582     285     282      93
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     438     678    1242 
   Size:        438     240     564

  Fragments arranged by size: 

                 564     438     240
 
XmnI GAAnn'nnTTC

Cuts at:      0     269     621    1242 
   Size:        269     352     621

  Fragments arranged by size: 

                 621     352     269
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI    AlwNI    ApaLI     ApoI    BanII
     BbsI Bme1580I     BpmI    BsaAI    BseRI  BsiHKAI Bsp1286I    BsrDI
   BstAPI     DraI   DraIII     EaeI  EcoICRI  Eco57MI     FalI    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI   MspA1I     NdeI     NspI
     PfoI     PstI    PvuII     SacI     ScaI    SexAI     SfcI     SpeI
     SspI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI