Restriction map for
pFN21AE5506
(Linear) (Six-base) MAPSORT of: KIEE5506.seq Check: 1715 from: 1 to: 1251
KIEE5506.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1251
Size: 490 761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 709 741 1251
Size: 61 32 616 32 510
Fragments arranged by size:
616 510 61 32 32
ApoI r'AATT_y
Cuts at: 0 630 756 1150 1216 1251
Size: 630 126 394 66 35
Fragments arranged by size:
630 394 126 66 35
BanI G'GyrC_C
Cuts at: 0 163 1251
Size: 163 1088
BanII G_rGCy'C
Cuts at: 0 823 1136 1251
Size: 823 313 115
Fragments arranged by size:
823 313 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 167 416 1251
Size: 167 249 835
Fragments arranged by size:
835 249 167
BfrBI ATG'CAT
Cuts at: 0 734 1251
Size: 734 517
BmrI ACTGGGnnnn_n'
Cuts at: 0 1101 1251
Size: 1101 150
BmtI G_CTAG'C
Cuts at: 0 1210 1251
Size: 1210 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1251
Size: 1222 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 423 546 1192 1251
Size: 423 123 646 59
Fragments arranged by size:
646 423 123 59
BsaAI yAC'GTr
Cuts at: 0 177 1251
Size: 177 1074
BsaBI GATnn'nnATC
Cuts at: 0 404 812 948 1251
Size: 404 408 136 303
Fragments arranged by size:
408 404 303 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 709 739 1251
Size: 61 30 618 30 512
Fragments arranged by size:
618 512 61 30 30
BseYI C'CCAG_C
Cuts at: 0 546 1251
Size: 546 705
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 824 1212 1251
Size: 809 15 388 39
Fragments arranged by size:
809 388 39 15
BsiHKAI G_wGCw'C
Cuts at: 0 1136 1251
Size: 1136 115
BsmI GAATG_Cn'
Cuts at: 0 521 550 1251
Size: 521 29 701
Fragments arranged by size:
701 521 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 838 1251
Size: 838 413
Bsp1286I G_dGCh'C
Cuts at: 0 823 1136 1251
Size: 823 313 115
Fragments arranged by size:
823 313 115
BspHI T'CATG_A
Cuts at: 0 279 1251
Size: 279 972
BssSI C'ACGA_G
Cuts at: 0 530 1251
Size: 530 721
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 1251
Size: 618 633
BstYI r'GATC_y
Cuts at: 0 436 1251
Size: 436 815
EaeI y'GGCC_r
Cuts at: 0 128 980 1251
Size: 128 852 271
Fragments arranged by size:
852 271 128
EarI CTCTTCn'nnn_
Cuts at: 0 287 1251
Size: 287 964
EcoICRI GAG'CTC
Cuts at: 0 1134 1251
Size: 1134 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1251
Size: 490 761
EcoRV GAT'ATC
Cuts at: 0 327 1073 1251
Size: 327 746 178
Fragments arranged by size:
746 327 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 267 299 524 556 690 722
Size: 205 32 30 32 225 32 134 32
Cuts at: 722 997 1029 1251
Size: 275 32 222
Fragments arranged by size:
275 225 222 205 134 32 32 32
32 32 30
MfeI C'AATT_G
Cuts at: 0 44 1251
Size: 44 1207
MscI TGG'CCA
Cuts at: 0 130 982 1251
Size: 130 852 269
Fragments arranged by size:
852 269 130
MslI CAynn'nnrTG
Cuts at: 0 534 881 1251
Size: 534 347 370
Fragments arranged by size:
534 370 347
NheI G'CTAG_C
Cuts at: 0 1206 1251
Size: 1206 45
NsiI A_TGCA'T
Cuts at: 0 736 1251
Size: 736 515
NspI r_CATG'y
Cuts at: 0 696 1251
Size: 696 555
PfoI T'CCnGG_A
Cuts at: 0 1174 1251
Size: 1174 77
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 709 741 1251
Size: 61 32 616 32 510
Fragments arranged by size:
616 510 61 32 32
SacI G_AGCT'C
Cuts at: 0 1136 1251
Size: 1136 115
SmlI C'TyrA_G
Cuts at: 0 1237 1251
Size: 1237 14
StyI C'CwwG_G
Cuts at: 0 242 1251
Size: 242 1009
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 638 1251
Size: 184 454 613
Fragments arranged by size:
613 454 184
XmnI GAAnn'nnTTC
Cuts at: 0 76 1251
Size: 76 1175
Enzymes that do cut:
AcuI AloI ApoI BanI BanII BciVI BfrBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstXI BstYI
EaeI EarI EcoICRI Eco57MI EcoRV Hin4I MfeI MscI
MslI NheI NsiI NspI PfoI PpiI SacI SmlI
StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BsaHI BsaWI BseRI BsiEI BsiWI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI