Restriction map for
pFN21AE5496
(Linear) (Six-base) MAPSORT of: KIEE5496.seq Check: 520 from: 1 to: 1050
KIEE5496.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
Acc65I G'GTAC_C
Cuts at: 0 321 1050
Size: 321 729
AflIII A'CryG_T
Cuts at: 0 808 1050
Size: 808 242
AleI CACnn'nnGTG
Cuts at: 0 415 1050
Size: 415 635
ApoI r'AATT_y
Cuts at: 0 491 1050
Size: 491 559
BanI G'GyrC_C
Cuts at: 0 321 1050
Size: 321 729
BanII G_rGCy'C
Cuts at: 0 263 715 1050
Size: 263 452 335
Fragments arranged by size:
452 335 263
BciVI GTATCCnnnnn_n'
Cuts at: 0 148 1050
Size: 148 902
BclI T'GATC_A
Cuts at: 0 935 1050
Size: 935 115
BfrBI ATG'CAT
Cuts at: 0 1017 1050
Size: 1017 33
Bme1580I G_kGCm'C
Cuts at: 0 671 899 1050
Size: 671 228 151
Fragments arranged by size:
671 228 151
BmtI G_CTAG'C
Cuts at: 0 922 1050
Size: 922 128
Bpu10I CC'TnA_GC
Cuts at: 0 348 1050
Size: 348 702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 739 1050
Size: 488 251 311
Fragments arranged by size:
488 311 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 1050
Size: 775 275
BsaAI yAC'GTr
Cuts at: 0 933 1050
Size: 933 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 765 1050
Size: 303 462 285
Fragments arranged by size:
462 303 285
BseYI C'CCAG_C
Cuts at: 0 81 513 670 942 1050
Size: 81 432 157 272 108
Fragments arranged by size:
432 272 157 108 81
BsiHKAI G_wGCw'C
Cuts at: 0 632 1050
Size: 632 418
BsmI GAATG_Cn'
Cuts at: 0 26 1050
Size: 26 1024
BsmBI CGTCTCn'nnnn_
Cuts at: 0 415 1050
Size: 415 635
Bsp1286I G_dGCh'C
Cuts at: 0 263 632 671 715 899 1050
Size: 263 369 39 44 184 151
Fragments arranged by size:
369 263 184 151 44 39
BsrDI GCAATG_nn'
Cuts at: 0 728 1050
Size: 728 322
BsrGI T'GTAC_A
Cuts at: 0 191 1050
Size: 191 859
BstAPI GCAn_nnn'nTGC
Cuts at: 0 344 1050
Size: 344 706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 569 1050
Size: 417 152 481
Fragments arranged by size:
481 417 152
BtgI C'CryG_G
Cuts at: 0 440 1050
Size: 440 610
DraIII CAC_nnn'GTG
Cuts at: 0 184 1050
Size: 184 866
EaeI y'GGCC_r
Cuts at: 0 527 641 1050
Size: 527 114 409
Fragments arranged by size:
527 409 114
EcoRI G'AATT_C
Cuts at: 0 491 1050
Size: 491 559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 1050
Size: 88 962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 564 596 1050
Size: 69 32 463 32 454
Fragments arranged by size:
463 454 69 32 32
HincII GTy'rAC
Cuts at: 0 7 1050
Size: 7 1043
KpnI G_GTAC'C
Cuts at: 0 325 1050
Size: 325 725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1050
Size: 550 500
MscI TGG'CCA
Cuts at: 0 529 643 1050
Size: 529 114 407
Fragments arranged by size:
529 407 114
MslI CAynn'nnrTG
Cuts at: 0 304 388 415 1050
Size: 304 84 27 635
Fragments arranged by size:
635 304 84 27
MspA1I CmG'CkG
Cuts at: 0 415 1050
Size: 415 635
NcoI C'CATG_G
Cuts at: 0 440 1050
Size: 440 610
NheI G'CTAG_C
Cuts at: 0 918 1050
Size: 918 132
NsiI A_TGCA'T
Cuts at: 0 1019 1050
Size: 1019 31
NspI r_CATG'y
Cuts at: 0 656 812 1021 1050
Size: 656 156 209 29
Fragments arranged by size:
656 209 156 29
PciI A'CATG_T
Cuts at: 0 808 1050
Size: 808 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1050
Size: 105 945
PsiI TTA'TAA
Cuts at: 0 95 1050
Size: 95 955
PvuII CAG'CTG
Cuts at: 0 415 1050
Size: 415 635
SfcI C'TryA_G
Cuts at: 0 681 1050
Size: 681 369
SmlI C'TyrA_G
Cuts at: 0 503 754 1050
Size: 503 251 296
Fragments arranged by size:
503 296 251
StyI C'CwwG_G
Cuts at: 0 440 1050
Size: 440 610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 922 1050
Size: 922 128
TatI w'GTAC_w
Cuts at: 0 191 839 1050
Size: 191 648 211
Fragments arranged by size:
648 211 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 939 971 1050
Size: 939 32 79
Fragments arranged by size:
939 79 32
Enzymes that do cut:
Acc65I AflIII AleI ApoI BanI BanII BciVI BclI
BfrBI Bme1580I BmtI Bpu10I BpuEI BsaI BsaAI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstAPI
BstXI BtgI DraIII EaeI EcoRI FalI Hin4I HincII
KpnI MmeI MscI MslI MspA1I NcoI NheI NsiI
NspI PciI PflMI PsiI PvuII SfcI SmlI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BglI BglII BlpI BmgBI BmrI BplI BpmI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI