(Linear) (Six-base) MAPSORT of: KIEE5494.seq  Check: 942  from: 1  to: 1044



KIEE5494.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AccI GT'mk_AC

Cuts at:      0     593    1044 
   Size:        593     451
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     840     994    1009    1044 
   Size:        840     154      15      35

  Fragments arranged by size: 

                 840     154      35      15
 
ApoI r'AATT_y

Cuts at:      0     103    1044 
   Size:        103     941
 
AvaI C'yCGr_G

Cuts at:      0      92    1044 
   Size:         92     952
 
BanII G_rGCy'C

Cuts at:      0     140    1044 
   Size:        140     904
 
BfrBI ATG'CAT

Cuts at:      0     833    1044 
   Size:        833     211
 
BglI GCCn_nnn'nGGC

Cuts at:      0     364    1044 
   Size:        364     680
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     697     747    1044 
   Size:        697      50     297

  Fragments arranged by size: 

                 697     297      50
 
BseYI C'CCAG_C

Cuts at:      0     114    1044 
   Size:        114     930
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     413     503    1044 
   Size:        140     273      90     541

  Fragments arranged by size: 

                 541     273     140      90
 
BsmI GAATG_Cn'

Cuts at:      0     553    1044 
   Size:        553     491
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     413     503    1044 
   Size:        140     273      90     541

  Fragments arranged by size: 

                 541     273     140      90
 
BspHI T'CATG_A

Cuts at:      0     476    1044 
   Size:        476     568
 
BsrBI CCG'CTC

Cuts at:      0     782    1044 
   Size:        782     262
 
BsrGI T'GTAC_A

Cuts at:      0     929    1044 
   Size:        929     115
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     549    1044 
   Size:        549     495
 
BtgI C'CryG_G

Cuts at:      0     422    1044 
   Size:        422     622
 
BtsI GCAGTG_nn'

Cuts at:      0     405    1044 
   Size:        405     639
 
ClaI AT'CG_AT

Cuts at:      0      70    1044 
   Size:         70     974
 
DraI TTT'AAA

Cuts at:      0     917    1044 
   Size:        917     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     394     970    1044 
   Size:        394     576      74

  Fragments arranged by size: 

                 576     394      74
 
EcoICRI GAG'CTC

Cuts at:      0     138    1044 
   Size:        138     906
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     840     994    1009    1044 
   Size:        840     154      15      35

  Fragments arranged by size: 

                 840     154      35      15
 
FspI TGC'GCA

Cuts at:      0     337    1044 
   Size:        337     707
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     969    1001    1044 
   Size:        969      32      43

  Fragments arranged by size: 

                 969      43      32
 
HincII GTy'rAC

Cuts at:      0     873    1044 
   Size:        873     171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     165    1044 
   Size:        165     879
 
MslI CAynn'nnrTG

Cuts at:      0     244     805     844    1044 
   Size:        244     561      39     200

  Fragments arranged by size: 

                 561     244     200      39
 
NcoI C'CATG_G

Cuts at:      0     422    1044 
   Size:        422     622
 
NsiI A_TGCA'T

Cuts at:      0     835    1044 
   Size:        835     209
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     577    1044 
   Size:        577     467
 
SacI G_AGCT'C

Cuts at:      0     140    1044 
   Size:        140     904
 
SfcI C'TryA_G

Cuts at:      0    1007    1044 
   Size:       1007      37
 
StyI C'CwwG_G

Cuts at:      0     422    1044 
   Size:        422     622
 
TatI w'GTAC_w

Cuts at:      0     248     929    1044 
   Size:        248     681     115

  Fragments arranged by size: 

                 681     248     115
 
Tth111I GACn'n_nGTC

Cuts at:      0     877    1044 
   Size:        877     167
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AvaI    BanII    BfrBI     BglI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrGI    BstXI
     BtgI     BtsI     ClaI     DraI     EarI  EcoICRI  Eco57MI     FspI
    Hin4I   HincII     MmeI     MslI     NcoI     NsiI    PflMI     SacI
     SfcI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI