Restriction map for
pFN21AE5493
(Linear) (Six-base) MAPSORT of: KIEE5493.seq Check: 5546 from: 1 to: 1041
KIEE5493.orf
With 182 enzymes: SgfI *
January 29, 2014 12:03 ..
AflIII A'CryG_T
Cuts at: 0 71 1041
Size: 71 970
ApoI r'AATT_y
Cuts at: 0 299 416 538 973 1041
Size: 299 117 122 435 68
Fragments arranged by size:
435 299 122 117 68
AvaI C'yCGr_G
Cuts at: 0 512 656 1041
Size: 512 144 385
Fragments arranged by size:
512 385 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 1041
Size: 215 33 793
Fragments arranged by size:
793 215 33
BamHI G'GATC_C
Cuts at: 0 446 1041
Size: 446 595
BanII G_rGCy'C
Cuts at: 0 457 1041
Size: 457 584
BbvCI CC'TCA_GC
Cuts at: 0 187 1041
Size: 187 854
BclI T'GATC_A
Cuts at: 0 792 1041
Size: 792 249
BglII A'GATC_T
Cuts at: 0 908 1041
Size: 908 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1041
Size: 710 331
Bpu10I CC'TnA_GC
Cuts at: 0 187 1041
Size: 187 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1041
Size: 515 526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 175 1041
Size: 130 45 866
Fragments arranged by size:
866 130 45
BseYI C'CCAG_C
Cuts at: 0 158 1041
Size: 158 883
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1041
Size: 207 834
BsmI GAATG_Cn'
Cuts at: 0 898 1041
Size: 898 143
Bsp1286I G_dGCh'C
Cuts at: 0 457 1041
Size: 457 584
BspHI T'CATG_A
Cuts at: 0 404 902 1041
Size: 404 498 139
Fragments arranged by size:
498 404 139
BsrDI GCAATG_nn'
Cuts at: 0 573 641 1034 1041
Size: 573 68 393 7
Fragments arranged by size:
573 393 68 7
BsrGI T'GTAC_A
Cuts at: 0 773 1031 1041
Size: 773 258 10
Fragments arranged by size:
773 258 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 1041
Size: 279 762
BstYI r'GATC_y
Cuts at: 0 446 685 908 1041
Size: 446 239 223 133
Fragments arranged by size:
446 239 223 133
EarI CTCTTCn'nnn_
Cuts at: 0 85 581 1041
Size: 85 496 460
Fragments arranged by size:
496 460 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1041
Size: 710 331
EcoRI G'AATT_C
Cuts at: 0 538 1041
Size: 538 503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 1041
Size: 261 780
HindIII A'AGCT_T
Cuts at: 0 707 1041
Size: 707 334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1041
Size: 53 988
MslI CAynn'nnrTG
Cuts at: 0 172 277 679 739 847 1041
Size: 172 105 402 60 108 194
Fragments arranged by size:
402 194 172 108 105 60
PfoI T'CCnGG_A
Cuts at: 0 442 1041
Size: 442 599
PspXI vC'TCGA_Gb
Cuts at: 0 656 1041
Size: 656 385
SfcI C'TryA_G
Cuts at: 0 458 479 867 1041
Size: 458 21 388 174
Fragments arranged by size:
458 388 174 21
SmlI C'TyrA_G
Cuts at: 0 656 1041
Size: 656 385
SspI AAT'ATT
Cuts at: 0 581 1041
Size: 581 460
StyI C'CwwG_G
Cuts at: 0 42 1041
Size: 42 999
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 1041
Size: 172 869
TatI w'GTAC_w
Cuts at: 0 176 749 773 1031 1041
Size: 176 573 24 258 10
Fragments arranged by size:
573 258 176 24 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 1041
Size: 59 32 950
Fragments arranged by size:
950 59 32
XhoI C'TCGA_G
Cuts at: 0 656 1041
Size: 656 385
XmnI GAAnn'nnTTC
Cuts at: 0 335 1041
Size: 335 706
Enzymes that do cut:
AflIII ApoI AvaI BaeI BamHI BanII BbvCI BclI
BglII BpmI Bpu10I BsaI BseRI BseYI BsgI BsmI
Bsp1286I BspHI BsrDI BsrGI BstXI BstYI EarI Eco57MI
EcoRI FalI HindIII MmeI MslI PfoI PspXI SfcI
SmlI SspI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BanI BbeI BbsI BcgI
BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XmaI ZraI